Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability
Abstract For over a century African swine fever (ASF) has been causing outbreaks leading to devastating losses for the swine industry. The current pandemic of ASF has shown no signs of stopping and continues to spread causing outbreaks in additional countries. Currently control relies mostly on cull...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-80170-2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559555909091328 |
|---|---|
| author | Nallely Espinoza Edward Spinard Ayushi Rai Elizabeth Ramirez-Medina Alyssa Valladares Amanda Meyers Manuel V. Borca Douglas P. Gladue |
| author_facet | Nallely Espinoza Edward Spinard Ayushi Rai Elizabeth Ramirez-Medina Alyssa Valladares Amanda Meyers Manuel V. Borca Douglas P. Gladue |
| author_sort | Nallely Espinoza |
| collection | DOAJ |
| description | Abstract For over a century African swine fever (ASF) has been causing outbreaks leading to devastating losses for the swine industry. The current pandemic of ASF has shown no signs of stopping and continues to spread causing outbreaks in additional countries. Currently control relies mostly on culling infected farms, and strict biosecurity procedures. Recently a vaccine, ASFV-G-ΔI177L was approved for use in Vietnam. In this study we evaluate the long-term stability of lyophilized ASFV-G-ΔI177L. Understanding the stability of different formulations of vaccines is information necessary for deployment of vaccines to ASF outbreak areas, particularly those that do not have a reliable well established cold chain to ensure conservation of vaccine quality. In this report, we determined that ASFV-G-ΔI177L, when lyophilized under specific conditions, is stable for up to one year at 4 °C, with similar vaccine titers after storage. Next-generation sequencing analysis also determined that lyophilization and long-term storage under these conditions had no effect on the genome of ASFV as the genome remained genetically identical to the original non-lyophilized form. |
| format | Article |
| id | doaj-art-f14eeb939d2145f9857cea2196ca4e08 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-f14eeb939d2145f9857cea2196ca4e082025-01-05T12:24:44ZengNature PortfolioScientific Reports2045-23222024-12-011411610.1038/s41598-024-80170-2Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stabilityNallely Espinoza0Edward Spinard1Ayushi Rai2Elizabeth Ramirez-Medina3Alyssa Valladares4Amanda Meyers5Manuel V. Borca6Douglas P. Gladue7Plum Island Animal Disease Center, Agricultural Research Service, USDAPlum Island Animal Disease Center, Agricultural Research Service, USDAPlum Island Animal Disease Center, Agricultural Research Service, USDAPlum Island Animal Disease Center, Agricultural Research Service, USDAPlum Island Animal Disease Center, Agricultural Research Service, USDAPlum Island Animal Disease Center, Agricultural Research Service, USDAPlum Island Animal Disease Center, Agricultural Research Service, USDAPlum Island Animal Disease Center, Agricultural Research Service, USDAAbstract For over a century African swine fever (ASF) has been causing outbreaks leading to devastating losses for the swine industry. The current pandemic of ASF has shown no signs of stopping and continues to spread causing outbreaks in additional countries. Currently control relies mostly on culling infected farms, and strict biosecurity procedures. Recently a vaccine, ASFV-G-ΔI177L was approved for use in Vietnam. In this study we evaluate the long-term stability of lyophilized ASFV-G-ΔI177L. Understanding the stability of different formulations of vaccines is information necessary for deployment of vaccines to ASF outbreak areas, particularly those that do not have a reliable well established cold chain to ensure conservation of vaccine quality. In this report, we determined that ASFV-G-ΔI177L, when lyophilized under specific conditions, is stable for up to one year at 4 °C, with similar vaccine titers after storage. Next-generation sequencing analysis also determined that lyophilization and long-term storage under these conditions had no effect on the genome of ASFV as the genome remained genetically identical to the original non-lyophilized form.https://doi.org/10.1038/s41598-024-80170-2ASFVVaccineLyophilizationAfrican swine fever |
| spellingShingle | Nallely Espinoza Edward Spinard Ayushi Rai Elizabeth Ramirez-Medina Alyssa Valladares Amanda Meyers Manuel V. Borca Douglas P. Gladue Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability Scientific Reports ASFV Vaccine Lyophilization African swine fever |
| title | Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability |
| title_full | Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability |
| title_fullStr | Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability |
| title_full_unstemmed | Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability |
| title_short | Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability |
| title_sort | lyophilization of asfv vaccine candidate asfv g δi177l offers long term stability |
| topic | ASFV Vaccine Lyophilization African swine fever |
| url | https://doi.org/10.1038/s41598-024-80170-2 |
| work_keys_str_mv | AT nallelyespinoza lyophilizationofasfvvaccinecandidateasfvgdi177lofferslongtermstability AT edwardspinard lyophilizationofasfvvaccinecandidateasfvgdi177lofferslongtermstability AT ayushirai lyophilizationofasfvvaccinecandidateasfvgdi177lofferslongtermstability AT elizabethramirezmedina lyophilizationofasfvvaccinecandidateasfvgdi177lofferslongtermstability AT alyssavalladares lyophilizationofasfvvaccinecandidateasfvgdi177lofferslongtermstability AT amandameyers lyophilizationofasfvvaccinecandidateasfvgdi177lofferslongtermstability AT manuelvborca lyophilizationofasfvvaccinecandidateasfvgdi177lofferslongtermstability AT douglaspgladue lyophilizationofasfvvaccinecandidateasfvgdi177lofferslongtermstability |