Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i>
Polycyclic aromatic hydrocarbons (PAHs) are known to have toxic effects on fish. In this study, we examined the effects of benz[a]anthracene (BaA), a type of PAH, on fish liver metabolism. Nibbler fish (<i>Girella punctata</i>) were intraperitoneally injected with BaA (10 ng/g body weigh...
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2024-12-01
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| author | Muhammad Ahya Rafiuddin Hajime Matsubara Kaito Hatano Masato Honda Kenji Toyota Kouhei Kuroda Keito Tsunoda Yukihiro Furusawa Yoshiaki Tabuchi Tetsushi Hirano Akihiro Sakatoku Chun-Sang Hong Ajai K. Srivastav Thumronk Amornsakun Nobuaki Shimizu Mohamed I. Zanaty Tatsuo Harumi Kohei Yamauchi Tamás Müller Ning Tang Atsuhiko Hattori Kazuichi Hayakawa Nobuo Suzuki |
| author_facet | Muhammad Ahya Rafiuddin Hajime Matsubara Kaito Hatano Masato Honda Kenji Toyota Kouhei Kuroda Keito Tsunoda Yukihiro Furusawa Yoshiaki Tabuchi Tetsushi Hirano Akihiro Sakatoku Chun-Sang Hong Ajai K. Srivastav Thumronk Amornsakun Nobuaki Shimizu Mohamed I. Zanaty Tatsuo Harumi Kohei Yamauchi Tamás Müller Ning Tang Atsuhiko Hattori Kazuichi Hayakawa Nobuo Suzuki |
| author_sort | Muhammad Ahya Rafiuddin |
| collection | DOAJ |
| description | Polycyclic aromatic hydrocarbons (PAHs) are known to have toxic effects on fish. In this study, we examined the effects of benz[a]anthracene (BaA), a type of PAH, on fish liver metabolism. Nibbler fish (<i>Girella punctata</i>) were intraperitoneally injected with BaA (10 ng/g body weight) four times over a 10-day period. BaA significantly decreased known bone metabolism-related plasma factors such as calcium and inorganic phosphorus. Moreover, significant reductions were observed in the plasma levels of known liver metabolism-related factors, including ferrous ions, total bile acids, total bilirubin, free bilirubin, aspartate aminotransferase, and alkaline phosphatase. Interestingly, mono-hydroxylated metabolites of BaA, such as 3 hydroxylbenz[a]anthracene (3-OHBaA), were detected in the bile of BaA-injected nibbler fish. This hydroxylated form of BaA was found in its free form, rather than conjugated with glucuronic acid or sulfuric acid. Due to the lack of whole-genome sequence data for the nibbler fish, two nibbler fish-specific apoptosis-related factors (TNF receptor superfamily member 1A: <i>tnfrsf1a</i> and TNF superfamily member 10: <i>tnfsf10</i>) were isolated by De novo RNA sequencing. In a liver tissue culture, 3-OHBaA (10<sup>−6</sup> M) significantly upregulated the expression of <i>tnfrsf1a</i> and <i>tnfsf10</i> in the liver. These results provide the first evidence that 3-OHBaA metabolites exhibit toxic effects on the liver in teleost. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-f1261a75391645a8b24f073aacca9a0a2024-12-27T14:56:47ZengMDPI AGToxics2305-63042024-12-01121291510.3390/toxics12120915Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i>Muhammad Ahya Rafiuddin0Hajime Matsubara1Kaito Hatano2Masato Honda3Kenji Toyota4Kouhei Kuroda5Keito Tsunoda6Yukihiro Furusawa7Yoshiaki Tabuchi8Tetsushi Hirano9Akihiro Sakatoku10Chun-Sang Hong11Ajai K. Srivastav12Thumronk Amornsakun13Nobuaki Shimizu14Mohamed I. Zanaty15Tatsuo Harumi16Kohei Yamauchi17Tamás Müller18Ning Tang19Atsuhiko Hattori20Kazuichi Hayakawa21Nobuo Suzuki22Noto Center for Fisheries Science and Technology, Kanazawa University, Ossaka, Noto-cho, Ishikawa 927-0552, JapanNoto Center for Fisheries Science and Technology, Kanazawa University, Ossaka, Noto-cho, Ishikawa 927-0552, JapanNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ogi, Noto-cho, Ishikawa 927-0553, JapanBotanical Garden, Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa, Ishikawa 920-1192, JapanDepartment of Bioresource Science, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima, Hiroshima 739-8528, JapanNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ogi, Noto-cho, Ishikawa 927-0553, JapanNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ogi, Noto-cho, Ishikawa 927-0553, JapanDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama 939-0398, JapanDivision of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani, Toyama 930-0194, JapanDivision of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani, Toyama 930-0194, JapanSchool of Science, Academic Assembly, University of Toyama, Gofuku, Toyama 930-8555, JapanGraduate School of Particulate Matter Management, Korea University, Seongbuk-gu, Seoul 02841, Republic of KoreaDepartment of Zoology, D.D.U. Gorakhpur University, Gorakhpur 273-009, IndiaFisheries Technology Program, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, ThailandNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ogi, Noto-cho, Ishikawa 927-0553, JapanBiotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Anatomy, Asahikawa Medical College, Asahikawa, Hokkaido 078-8510, JapanNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ogi, Noto-cho, Ishikawa 927-0553, JapanDepartment of Freshwater Fish Ecology, Institute of Aquaculture and Environmental Safety, Szent István Campus, Hungarian University of Agriculture and Life Sciences, 2100 Godollo, HungaryInstitute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Ishikawa 920-1192, JapanDepartment of Sport and Wellness, College of Sport and Wellness, Rikkyo University, Niiza, Saitama 352-8558, JapanLow Level Radioactivity Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Nomi, Ishikawa 923-1224, JapanNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ogi, Noto-cho, Ishikawa 927-0553, JapanPolycyclic aromatic hydrocarbons (PAHs) are known to have toxic effects on fish. In this study, we examined the effects of benz[a]anthracene (BaA), a type of PAH, on fish liver metabolism. Nibbler fish (<i>Girella punctata</i>) were intraperitoneally injected with BaA (10 ng/g body weight) four times over a 10-day period. BaA significantly decreased known bone metabolism-related plasma factors such as calcium and inorganic phosphorus. Moreover, significant reductions were observed in the plasma levels of known liver metabolism-related factors, including ferrous ions, total bile acids, total bilirubin, free bilirubin, aspartate aminotransferase, and alkaline phosphatase. Interestingly, mono-hydroxylated metabolites of BaA, such as 3 hydroxylbenz[a]anthracene (3-OHBaA), were detected in the bile of BaA-injected nibbler fish. This hydroxylated form of BaA was found in its free form, rather than conjugated with glucuronic acid or sulfuric acid. Due to the lack of whole-genome sequence data for the nibbler fish, two nibbler fish-specific apoptosis-related factors (TNF receptor superfamily member 1A: <i>tnfrsf1a</i> and TNF superfamily member 10: <i>tnfsf10</i>) were isolated by De novo RNA sequencing. In a liver tissue culture, 3-OHBaA (10<sup>−6</sup> M) significantly upregulated the expression of <i>tnfrsf1a</i> and <i>tnfsf10</i> in the liver. These results provide the first evidence that 3-OHBaA metabolites exhibit toxic effects on the liver in teleost.https://www.mdpi.com/2305-6304/12/12/915benz[a]anthracenehydroxylated-benz[a]anthraceneapoptosis-related factorsbiomarkers<i>Girella punctata</i> |
| spellingShingle | Muhammad Ahya Rafiuddin Hajime Matsubara Kaito Hatano Masato Honda Kenji Toyota Kouhei Kuroda Keito Tsunoda Yukihiro Furusawa Yoshiaki Tabuchi Tetsushi Hirano Akihiro Sakatoku Chun-Sang Hong Ajai K. Srivastav Thumronk Amornsakun Nobuaki Shimizu Mohamed I. Zanaty Tatsuo Harumi Kohei Yamauchi Tamás Müller Ning Tang Atsuhiko Hattori Kazuichi Hayakawa Nobuo Suzuki Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i> Toxics benz[a]anthracene hydroxylated-benz[a]anthracene apoptosis-related factors biomarkers <i>Girella punctata</i> |
| title | Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i> |
| title_full | Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i> |
| title_fullStr | Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i> |
| title_full_unstemmed | Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i> |
| title_short | Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i> |
| title_sort | hydroxylated benz a anthracenes induce two apoptosis related gene expressions in the liver of the nibbler fish i girella punctata i |
| topic | benz[a]anthracene hydroxylated-benz[a]anthracene apoptosis-related factors biomarkers <i>Girella punctata</i> |
| url | https://www.mdpi.com/2305-6304/12/12/915 |
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