Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i>

Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish <i>Girella punctata</i>

Polycyclic aromatic hydrocarbons (PAHs) are known to have toxic effects on fish. In this study, we examined the effects of benz[a]anthracene (BaA), a type of PAH, on fish liver metabolism. Nibbler fish (<i>Girella punctata</i>) were intraperitoneally injected with BaA (10 ng/g body weigh...

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Main Authors: Muhammad Ahya Rafiuddin, Hajime Matsubara, Kaito Hatano, Masato Honda, Kenji Toyota, Kouhei Kuroda, Keito Tsunoda, Yukihiro Furusawa, Yoshiaki Tabuchi, Tetsushi Hirano, Akihiro Sakatoku, Chun-Sang Hong, Ajai K. Srivastav, Thumronk Amornsakun, Nobuaki Shimizu, Mohamed I. Zanaty, Tatsuo Harumi, Kohei Yamauchi, Tamás Müller, Ning Tang, Atsuhiko Hattori, Kazuichi Hayakawa, Nobuo Suzuki
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Toxics
Subjects:
benz[a]anthracene
hydroxylated-benz[a]anthracene
apoptosis-related factors
biomarkers
<i>Girella punctata</i>
Online Access:https://www.mdpi.com/2305-6304/12/12/915
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Summary:Polycyclic aromatic hydrocarbons (PAHs) are known to have toxic effects on fish. In this study, we examined the effects of benz[a]anthracene (BaA), a type of PAH, on fish liver metabolism. Nibbler fish (<i>Girella punctata</i>) were intraperitoneally injected with BaA (10 ng/g body weight) four times over a 10-day period. BaA significantly decreased known bone metabolism-related plasma factors such as calcium and inorganic phosphorus. Moreover, significant reductions were observed in the plasma levels of known liver metabolism-related factors, including ferrous ions, total bile acids, total bilirubin, free bilirubin, aspartate aminotransferase, and alkaline phosphatase. Interestingly, mono-hydroxylated metabolites of BaA, such as 3 hydroxylbenz[a]anthracene (3-OHBaA), were detected in the bile of BaA-injected nibbler fish. This hydroxylated form of BaA was found in its free form, rather than conjugated with glucuronic acid or sulfuric acid. Due to the lack of whole-genome sequence data for the nibbler fish, two nibbler fish-specific apoptosis-related factors (TNF receptor superfamily member 1A: <i>tnfrsf1a</i> and TNF superfamily member 10: <i>tnfsf10</i>) were isolated by De novo RNA sequencing. In a liver tissue culture, 3-OHBaA (10<sup>−6</sup> M) significantly upregulated the expression of <i>tnfrsf1a</i> and <i>tnfsf10</i> in the liver. These results provide the first evidence that 3-OHBaA metabolites exhibit toxic effects on the liver in teleost.
ISSN:2305-6304

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