Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers
Purpose. [18F]F-AraG is a radiolabeled nucleoside analog that shows relative specificity for activated T cells. The aim of this study was to investigate the biodistribution of [18F]F-AraG in healthy volunteers and assess the preliminary safety and radiation dosimetry. Methods. Six healthy subjects (...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-01-01
|
| Series: | Molecular Imaging |
| Online Access: | http://dx.doi.org/10.1155/2022/3667417 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841564609625980928 |
|---|---|
| author | Jelena Levi Heying Duan Shahriar Yaghoubi Juliet Packiasamy Lyna Huynh Tina Lam Faiq Shaikh Deepak Behera Hong Song Joseph Blecha Salma Jivan Youngho Seo Henry F. VanBrocklin |
| author_facet | Jelena Levi Heying Duan Shahriar Yaghoubi Juliet Packiasamy Lyna Huynh Tina Lam Faiq Shaikh Deepak Behera Hong Song Joseph Blecha Salma Jivan Youngho Seo Henry F. VanBrocklin |
| author_sort | Jelena Levi |
| collection | DOAJ |
| description | Purpose. [18F]F-AraG is a radiolabeled nucleoside analog that shows relative specificity for activated T cells. The aim of this study was to investigate the biodistribution of [18F]F-AraG in healthy volunteers and assess the preliminary safety and radiation dosimetry. Methods. Six healthy subjects (three female and three male) between the ages of 24 and 60 participated in the study. Each subject received a bolus venous injection of [18F]F-AraG (dose range: 244.2–329.3 MBq) prior to four consecutive PET/MR whole-body scans. Blood samples were collected at regular intervals and vital signs monitored before and after tracer administration. Regions of interest were delineated for multiple organs, and the area under the time-activity curves was calculated for each organ and used to derive time-integrated activity coefficient (TIAC). TIACs were input for absorbed dose and effective dose calculations using OLINDA. Results. PET/MR examination was well tolerated, and no adverse effects to the administration of [18F]F-AraG were noted by the study participants. The biodistribution was generally reflective of the expression and activity profiles of the enzymes involved in [18F]F-AraG’s cellular accumulation, mitochondrial kinase dGK, and SAMHD1. The highest uptake was observed in the kidneys and liver, while the brain, lung, bone marrow, and muscle showed low tracer uptake. The estimated effective dose for [18F]F-AraG was 0.0162 mSv/MBq (0.0167 mSv/MBq for females and 0.0157 mSv/MBq for males). Conclusion. Biodistribution of [18F]F-AraG in healthy volunteers was consistent with its association with mitochondrial metabolism. PET/MR [18F]F-AraG imaging was well tolerated, with a radiation dosimetry profile similar to other commonly used [18F]-labeled tracers. [18F]F-AraG’s connection with mitochondrial biogenesis and favorable biodistribution characteristics make it an attractive tracer with a variety of potential applications. |
| format | Article |
| id | doaj-art-f0bbedca78fb42b38cf3884b3981ae2d |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-f0bbedca78fb42b38cf3884b3981ae2d2025-01-02T22:37:52ZengSAGE PublishingMolecular Imaging1536-01212022-01-01202210.1155/2022/3667417Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy VolunteersJelena Levi0Heying Duan1Shahriar Yaghoubi2Juliet Packiasamy3Lyna Huynh4Tina Lam5Faiq Shaikh6Deepak Behera7Hong Song8Joseph Blecha9Salma Jivan10Youngho Seo11Henry F. VanBrocklin12CellSight Technologies IncorporatedDepartment of RadiologyCellSight Technologies IncorporatedCellSight Technologies IncorporatedCellSight Technologies IncorporatedCellSight Technologies IncorporatedCellSight Technologies IncorporatedCellSight Technologies IncorporatedDepartment of RadiologyDepartment of Radiology and Biomedical ImagingDepartment of Radiology and Biomedical ImagingDepartment of Radiology and Biomedical ImagingDepartment of Radiology and Biomedical ImagingPurpose. [18F]F-AraG is a radiolabeled nucleoside analog that shows relative specificity for activated T cells. The aim of this study was to investigate the biodistribution of [18F]F-AraG in healthy volunteers and assess the preliminary safety and radiation dosimetry. Methods. Six healthy subjects (three female and three male) between the ages of 24 and 60 participated in the study. Each subject received a bolus venous injection of [18F]F-AraG (dose range: 244.2–329.3 MBq) prior to four consecutive PET/MR whole-body scans. Blood samples were collected at regular intervals and vital signs monitored before and after tracer administration. Regions of interest were delineated for multiple organs, and the area under the time-activity curves was calculated for each organ and used to derive time-integrated activity coefficient (TIAC). TIACs were input for absorbed dose and effective dose calculations using OLINDA. Results. PET/MR examination was well tolerated, and no adverse effects to the administration of [18F]F-AraG were noted by the study participants. The biodistribution was generally reflective of the expression and activity profiles of the enzymes involved in [18F]F-AraG’s cellular accumulation, mitochondrial kinase dGK, and SAMHD1. The highest uptake was observed in the kidneys and liver, while the brain, lung, bone marrow, and muscle showed low tracer uptake. The estimated effective dose for [18F]F-AraG was 0.0162 mSv/MBq (0.0167 mSv/MBq for females and 0.0157 mSv/MBq for males). Conclusion. Biodistribution of [18F]F-AraG in healthy volunteers was consistent with its association with mitochondrial metabolism. PET/MR [18F]F-AraG imaging was well tolerated, with a radiation dosimetry profile similar to other commonly used [18F]-labeled tracers. [18F]F-AraG’s connection with mitochondrial biogenesis and favorable biodistribution characteristics make it an attractive tracer with a variety of potential applications.http://dx.doi.org/10.1155/2022/3667417 |
| spellingShingle | Jelena Levi Heying Duan Shahriar Yaghoubi Juliet Packiasamy Lyna Huynh Tina Lam Faiq Shaikh Deepak Behera Hong Song Joseph Blecha Salma Jivan Youngho Seo Henry F. VanBrocklin Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers Molecular Imaging |
| title | Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers |
| title_full | Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers |
| title_fullStr | Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers |
| title_full_unstemmed | Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers |
| title_short | Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers |
| title_sort | biodistribution of a mitochondrial metabolic tracer 18f f arag in healthy volunteers |
| url | http://dx.doi.org/10.1155/2022/3667417 |
| work_keys_str_mv | AT jelenalevi biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT heyingduan biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT shahriaryaghoubi biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT julietpackiasamy biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT lynahuynh biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT tinalam biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT faiqshaikh biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT deepakbehera biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT hongsong biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT josephblecha biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT salmajivan biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT younghoseo biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers AT henryfvanbrocklin biodistributionofamitochondrialmetabolictracer18ffaraginhealthyvolunteers |