Transcriptome-based protein-protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to LPS stimulation in Amphioctopus fangsiao
One of the major challenges in the aquaculture of marine organisms is the susceptibility to pathogens. Immune cells defend bacterial invasion by recognizing pathogen-associated molecular patterns (PAMPs) such as Lipopolysaccharides (LPS) in Gram-negative bacteria. The white body are key organs for i...
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Elsevier
2024-12-01
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| Series: | Comparative Immunology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950311624000181 |
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| author | Zhengcai Lu Yancheng Zhao Tingjin Lv Xipan Chen Cuiju Cui Xiumei Liu Zan Li Liyong Wang Xiaohui Xu Jianmin Yang |
| author_facet | Zhengcai Lu Yancheng Zhao Tingjin Lv Xipan Chen Cuiju Cui Xiumei Liu Zan Li Liyong Wang Xiaohui Xu Jianmin Yang |
| author_sort | Zhengcai Lu |
| collection | DOAJ |
| description | One of the major challenges in the aquaculture of marine organisms is the susceptibility to pathogens. Immune cells defend bacterial invasion by recognizing pathogen-associated molecular patterns (PAMPs) such as Lipopolysaccharides (LPS) in Gram-negative bacteria. The white body are key organs for immune response and hematopoiesis, and play an important role in understanding the antimicrobial biological response in gold-ringed octopus (Amphioctopus fangsiao). In the present research, transcriptome sequencing and bioinformatics analysis were conducted on A. fangsiao white body tissue after 6 h and 24 h of LPS stimulation. The results showed 2,029 and 73 differentially expressed genes (DEGs) at the 6- and 24-hour time points, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were beneficial in determining immune-related terms and signaling pathways. Subsequently, a protein-protein interaction (PPI) network was constructed and 20 hub genes, such as AKT3, MAPK14, and PIK3CA, were identified. These genes are involved in a variety of signaling pathways, including the Hippo signaling pathway, chemokine signaling pathway, and leukocyte trans endothelial migration. Using qRT-PCR, the accuracy of the 20 hub genes was verified. The present study provides a substantial theoretical foundation for the research on A. fangsiao white body immunity, enhancing the current understanding of cephalopods' innate immunity. |
| format | Article |
| id | doaj-art-f07bda7741b6441ea4b7012e84dd8263 |
| institution | Kabale University |
| issn | 2950-3116 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Comparative Immunology Reports |
| spelling | doaj-art-f07bda7741b6441ea4b7012e84dd82632024-12-18T08:55:59ZengElsevierComparative Immunology Reports2950-31162024-12-017200151Transcriptome-based protein-protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to LPS stimulation in Amphioctopus fangsiaoZhengcai Lu0Yancheng Zhao1Tingjin Lv2Xipan Chen3Cuiju Cui4Xiumei Liu5Zan Li6Liyong Wang7Xiaohui Xu8Jianmin Yang9School of Agriculture, Ludong University, Yantai 264025, PR ChinaSchool of Agriculture, Ludong University, Yantai 264025, PR ChinaYantai Institute of Marine Economy, Yantai 264003, PR ChinaSchool of Agriculture, Ludong University, Yantai 264025, PR ChinaSchool of Agriculture, Ludong University, Yantai 264025, PR ChinaCollege of Life Sciences, Yantai University, Yantai 264005, PR ChinaSchool of Agriculture, Ludong University, Yantai 264025, PR China; Corresponding author.Yantai Marine Economic Research Institute, Yantai 264003, PR China; Corresponding author.School of Agriculture, Ludong University, Yantai 264025, PR China; Corresponding author.School of Agriculture, Ludong University, Yantai 264025, PR ChinaOne of the major challenges in the aquaculture of marine organisms is the susceptibility to pathogens. Immune cells defend bacterial invasion by recognizing pathogen-associated molecular patterns (PAMPs) such as Lipopolysaccharides (LPS) in Gram-negative bacteria. The white body are key organs for immune response and hematopoiesis, and play an important role in understanding the antimicrobial biological response in gold-ringed octopus (Amphioctopus fangsiao). In the present research, transcriptome sequencing and bioinformatics analysis were conducted on A. fangsiao white body tissue after 6 h and 24 h of LPS stimulation. The results showed 2,029 and 73 differentially expressed genes (DEGs) at the 6- and 24-hour time points, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were beneficial in determining immune-related terms and signaling pathways. Subsequently, a protein-protein interaction (PPI) network was constructed and 20 hub genes, such as AKT3, MAPK14, and PIK3CA, were identified. These genes are involved in a variety of signaling pathways, including the Hippo signaling pathway, chemokine signaling pathway, and leukocyte trans endothelial migration. Using qRT-PCR, the accuracy of the 20 hub genes was verified. The present study provides a substantial theoretical foundation for the research on A. fangsiao white body immunity, enhancing the current understanding of cephalopods' innate immunity.http://www.sciencedirect.com/science/article/pii/S2950311624000181Amphioctopus fangsiaoLPSInnate immunityProtein-protein interaction networksWhite bodyRNA-seq |
| spellingShingle | Zhengcai Lu Yancheng Zhao Tingjin Lv Xipan Chen Cuiju Cui Xiumei Liu Zan Li Liyong Wang Xiaohui Xu Jianmin Yang Transcriptome-based protein-protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to LPS stimulation in Amphioctopus fangsiao Comparative Immunology Reports Amphioctopus fangsiao LPS Innate immunity Protein-protein interaction networks White body RNA-seq |
| title | Transcriptome-based protein-protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to LPS stimulation in Amphioctopus fangsiao |
| title_full | Transcriptome-based protein-protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to LPS stimulation in Amphioctopus fangsiao |
| title_fullStr | Transcriptome-based protein-protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to LPS stimulation in Amphioctopus fangsiao |
| title_full_unstemmed | Transcriptome-based protein-protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to LPS stimulation in Amphioctopus fangsiao |
| title_short | Transcriptome-based protein-protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to LPS stimulation in Amphioctopus fangsiao |
| title_sort | transcriptome based protein protein interaction analysis reveals immune gene network elucidating white body immunity mechanisms in response to lps stimulation in amphioctopus fangsiao |
| topic | Amphioctopus fangsiao LPS Innate immunity Protein-protein interaction networks White body RNA-seq |
| url | http://www.sciencedirect.com/science/article/pii/S2950311624000181 |
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