Spatiotemporal regulation of alginate sub-structures at multiple scales revealed by monoclonal antibodies
Alginates are abundant linear polysaccharides produced by brown algae and some bacteria. They have multiple biological roles and important medical and commercial uses. Alginates are comprised of D-mannuronic acid (M) and L-guluronic acid (G) and the ratios and distribution patterns of M and G profou...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | The Cell Surface |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468233024000185 |
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| author | Catherine T. Jones Cassie Bakshani Ieva Lelenaite Jozef Mravec Stjepan Krešimir Kračun Jeff Pearson Mathew D. Wilcox Kevin Hardouin Sonia Kridi Cécile Hervé William G.T. Willats |
| author_facet | Catherine T. Jones Cassie Bakshani Ieva Lelenaite Jozef Mravec Stjepan Krešimir Kračun Jeff Pearson Mathew D. Wilcox Kevin Hardouin Sonia Kridi Cécile Hervé William G.T. Willats |
| author_sort | Catherine T. Jones |
| collection | DOAJ |
| description | Alginates are abundant linear polysaccharides produced by brown algae and some bacteria. They have multiple biological roles and important medical and commercial uses. Alginates are comprised of D-mannuronic acid (M) and L-guluronic acid (G) and the ratios and distribution patterns of M and G profoundly impact their physiological and rheological properties. The structure/function relationships of alginates have been extensively studied in vitro but our understanding of the in vivo spatiotemporal regulation of alginate fine structures and their biological implications is limited. Monoclonal antibodies (mAbs) are powerful tools for localising and quantifying glycan structures and several alginate-directed mAbs have been developed. We used a library of well-defined alginates, with M and G block ratios determined by NMR, to refine our understanding of the binding properties of alginate-directed mAbs. Using these probes, we obtained new insight into how structural features of alginates are regulated at different scales, from cellular to seasonal. |
| format | Article |
| id | doaj-art-f05b95c3e457411784b5ca30ca04c981 |
| institution | Kabale University |
| issn | 2468-2330 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Cell Surface |
| spelling | doaj-art-f05b95c3e457411784b5ca30ca04c9812025-01-04T04:56:46ZengElsevierThe Cell Surface2468-23302025-06-0113100136Spatiotemporal regulation of alginate sub-structures at multiple scales revealed by monoclonal antibodiesCatherine T. Jones0Cassie Bakshani1Ieva Lelenaite2Jozef Mravec3Stjepan Krešimir Kračun4Jeff Pearson5Mathew D. Wilcox6Kevin Hardouin7Sonia Kridi8Cécile Hervé9William G.T. Willats10School of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK; Current Affiliation: Leica Biosystems Newcastle Ltd, Balliol Business Park, Benton Lane, Newcastle Upon Tyne NE12 8EW, UKSchool of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK; Current Affiliation: Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UKSchool of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne NE1 7RU, UKDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Denmark; Current Affiliation: Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Akademická 2, 94901 Nitra, SlovakiaDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Denmark; Current Affiliation: Novonesis A/S, Gammel Venlighedsvej, 142970 Hørsholm, Denmark.Newcastle University Biosciences Institute, The Medical School, University of Newcastle Upon Tyne Framlington Place, Newcastle upon Tyne NE2 4HH, UKNewcastle University Biosciences Institute, The Medical School, University of Newcastle Upon Tyne Framlington Place, Newcastle upon Tyne NE2 4HH, UKCNRS / Sorbonne Université, Station Biologique De Roscoff, Place Georges Teissier, 29680 Roscoff, FranceCNRS / Sorbonne Université, Station Biologique De Roscoff, Place Georges Teissier, 29680 Roscoff, FranceCNRS / Sorbonne Université, Station Biologique De Roscoff, Place Georges Teissier, 29680 Roscoff, FranceSchool of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK; Corresponding author.Alginates are abundant linear polysaccharides produced by brown algae and some bacteria. They have multiple biological roles and important medical and commercial uses. Alginates are comprised of D-mannuronic acid (M) and L-guluronic acid (G) and the ratios and distribution patterns of M and G profoundly impact their physiological and rheological properties. The structure/function relationships of alginates have been extensively studied in vitro but our understanding of the in vivo spatiotemporal regulation of alginate fine structures and their biological implications is limited. Monoclonal antibodies (mAbs) are powerful tools for localising and quantifying glycan structures and several alginate-directed mAbs have been developed. We used a library of well-defined alginates, with M and G block ratios determined by NMR, to refine our understanding of the binding properties of alginate-directed mAbs. Using these probes, we obtained new insight into how structural features of alginates are regulated at different scales, from cellular to seasonal.http://www.sciencedirect.com/science/article/pii/S2468233024000185AlginateMonoclonal antibodiesAlgaeAlgal cell wallsNMR |
| spellingShingle | Catherine T. Jones Cassie Bakshani Ieva Lelenaite Jozef Mravec Stjepan Krešimir Kračun Jeff Pearson Mathew D. Wilcox Kevin Hardouin Sonia Kridi Cécile Hervé William G.T. Willats Spatiotemporal regulation of alginate sub-structures at multiple scales revealed by monoclonal antibodies The Cell Surface Alginate Monoclonal antibodies Algae Algal cell walls NMR |
| title | Spatiotemporal regulation of alginate sub-structures at multiple scales revealed by monoclonal antibodies |
| title_full | Spatiotemporal regulation of alginate sub-structures at multiple scales revealed by monoclonal antibodies |
| title_fullStr | Spatiotemporal regulation of alginate sub-structures at multiple scales revealed by monoclonal antibodies |
| title_full_unstemmed | Spatiotemporal regulation of alginate sub-structures at multiple scales revealed by monoclonal antibodies |
| title_short | Spatiotemporal regulation of alginate sub-structures at multiple scales revealed by monoclonal antibodies |
| title_sort | spatiotemporal regulation of alginate sub structures at multiple scales revealed by monoclonal antibodies |
| topic | Alginate Monoclonal antibodies Algae Algal cell walls NMR |
| url | http://www.sciencedirect.com/science/article/pii/S2468233024000185 |
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