The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental study
The objective was to study the effect of the delivery of exogenous nitric oxide on the severity of apoptosis, pyroptosis, and necroptosis of the renal parenchyma after simulating cardiopulmonary bypass and cardiopulmonary bypass with circulatory arrest.Materials and Methods. 24 Altai breed rams were...
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2024-06-01
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| Series: | Вестник анестезиологии и реаниматологии |
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| Online Access: | https://www.vair-journal.com/jour/article/view/990 |
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| author | M. A. Tyo N. O. Kamenshchikov Yu. K. Podoksenov A. V. Mukhomedzyanov L. N. Maslov I. V. Kravchenko E. A. Churilina B. N. Kozlov |
| author_facet | M. A. Tyo N. O. Kamenshchikov Yu. K. Podoksenov A. V. Mukhomedzyanov L. N. Maslov I. V. Kravchenko E. A. Churilina B. N. Kozlov |
| author_sort | M. A. Tyo |
| collection | DOAJ |
| description | The objective was to study the effect of the delivery of exogenous nitric oxide on the severity of apoptosis, pyroptosis, and necroptosis of the renal parenchyma after simulating cardiopulmonary bypass and cardiopulmonary bypass with circulatory arrest.Materials and Methods. 24 Altai breed rams were randomized into 4 equal groups. In the CPB and CPB+NO groups, we simulated cardiopulmonary bypass without circulatory arrest. In the CPB+CA and CPB+CA+NO groups, we simulated cardiopulmonary bypass with circulatory arrest. In the CPB+NO, CPB+CA+NO groups, NO was given perioperative in concentration of 80 ppm. In the CPB, CPB+CA groups, we supplied a standard oxygen-air mixture without NO.Results. In the CPB+CA+NO group, the TNF-α concentration was statistically significantly lower: 899 [739; 1019] ng/g compared to the CPB+CA group 1716 [1284; 2201] ng/g, p = 0.026. The remaining markers of programmed cell death did not differ between groups.Conclusions. Perioperative nitric oxide delivery reduces the expression of the extrinsic pathway of apoptosis of renal parenchyma cells in simulating operations with artificial circulation and circulatory arrest. Perioperative nitric oxide delivery at a dose of 80 ppm does not increase the processes of apoptosis, pyroptosis, and necroptosis in renal parenchyma. |
| format | Article |
| id | doaj-art-eff54d22060e4ddf8a4a06d3ddd0b4d9 |
| institution | Kabale University |
| issn | 2078-5658 2541-8653 |
| language | Russian |
| publishDate | 2024-06-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Вестник анестезиологии и реаниматологии |
| spelling | doaj-art-eff54d22060e4ddf8a4a06d3ddd0b4d92025-08-20T03:56:34ZrusNew Terra Publishing HouseВестник анестезиологии и реаниматологии2078-56582541-86532024-06-01213263310.24884/2078-5658-2024-21-3-26-33680The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental studyM. A. Tyo0N. O. Kamenshchikov1Yu. K. Podoksenov2A. V. Mukhomedzyanov3L. N. Maslov4I. V. Kravchenko5E. A. Churilina6B. N. Kozlov7Cardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center of the Russian Academy of SciencesThe objective was to study the effect of the delivery of exogenous nitric oxide on the severity of apoptosis, pyroptosis, and necroptosis of the renal parenchyma after simulating cardiopulmonary bypass and cardiopulmonary bypass with circulatory arrest.Materials and Methods. 24 Altai breed rams were randomized into 4 equal groups. In the CPB and CPB+NO groups, we simulated cardiopulmonary bypass without circulatory arrest. In the CPB+CA and CPB+CA+NO groups, we simulated cardiopulmonary bypass with circulatory arrest. In the CPB+NO, CPB+CA+NO groups, NO was given perioperative in concentration of 80 ppm. In the CPB, CPB+CA groups, we supplied a standard oxygen-air mixture without NO.Results. In the CPB+CA+NO group, the TNF-α concentration was statistically significantly lower: 899 [739; 1019] ng/g compared to the CPB+CA group 1716 [1284; 2201] ng/g, p = 0.026. The remaining markers of programmed cell death did not differ between groups.Conclusions. Perioperative nitric oxide delivery reduces the expression of the extrinsic pathway of apoptosis of renal parenchyma cells in simulating operations with artificial circulation and circulatory arrest. Perioperative nitric oxide delivery at a dose of 80 ppm does not increase the processes of apoptosis, pyroptosis, and necroptosis in renal parenchyma.https://www.vair-journal.com/jour/article/view/990nitric oxideapoptosisnecroptosispyroptosiscardiopulmonary bypass |
| spellingShingle | M. A. Tyo N. O. Kamenshchikov Yu. K. Podoksenov A. V. Mukhomedzyanov L. N. Maslov I. V. Kravchenko E. A. Churilina B. N. Kozlov The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental study Вестник анестезиологии и реаниматологии nitric oxide apoptosis necroptosis pyroptosis cardiopulmonary bypass |
| title | The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental study |
| title_full | The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental study |
| title_fullStr | The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental study |
| title_full_unstemmed | The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental study |
| title_short | The influence of nitric oxide delivery on the processes of apoptosis, necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass: an experimental study |
| title_sort | influence of nitric oxide delivery on the processes of apoptosis necroptosis and pyroptosis in the renal parenchyma after simulating cardiopulmonary bypass an experimental study |
| topic | nitric oxide apoptosis necroptosis pyroptosis cardiopulmonary bypass |
| url | https://www.vair-journal.com/jour/article/view/990 |
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