Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics
Background and Aim: Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled...
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Veterinary World
2025-01-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.18/January-2025/12.pdf |
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| author | Athhar Manabi Diansyah Sanstoso Santoso Herdis Herdis Muhammad Yusuf Tri Puji Priyatno Tulus Maulana Abdul Latief Toleng Muhammad Ihsan Andi Dagong Syahruddin Said Hikmayani Iskandar Aeni Nurlatifah Puji Lestari Lukman Affandy Abdullah Baharun |
| author_facet | Athhar Manabi Diansyah Sanstoso Santoso Herdis Herdis Muhammad Yusuf Tri Puji Priyatno Tulus Maulana Abdul Latief Toleng Muhammad Ihsan Andi Dagong Syahruddin Said Hikmayani Iskandar Aeni Nurlatifah Puji Lestari Lukman Affandy Abdullah Baharun |
| author_sort | Athhar Manabi Diansyah |
| collection | DOAJ |
| description | Background and Aim: Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled bulls.
Materials and Methods: Semen from five Bali-polled bulls was collected biweekly over five batches using artificial vaginas. Sperm kinematics were assessed using computer-assisted semen analysis (CASA). Fertility was evaluated through service per conception (S/C) in artificial insemination trials. Seminal plasma proteins were analyzed through liquid chromatography–tandem mass spectrometry (LC-MS/MS) and annotated using the UniProt database, PANTHER for gene ontology, and STRING database for protein interactions.
Results: Post-thaw sperm kinematics showed satisfactory results, with a mean S/C of 1.52. Proteomic analysis identified 138 proteins, including six (TEX101, BSP1, PRSS55, BSP3, SPADH2, and TPPP2) linked to sperm motility. These proteins were involved in key biological processes such as sperm capacitation, motility regulation, and sperm-oocyte interaction.
Conclusion: Sperm kinematics and seminal plasma proteomics provide insights into Bali-polled bull fertility. Identified proteins can serve as fertility biomarkers, aiding in superior local breed development and reproductive efficiency improvement. |
| format | Article |
| id | doaj-art-efd14d1c1dcb46e5806d1ec5ea32a8c1 |
| institution | Kabale University |
| issn | 0972-8988 2231-0916 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj-art-efd14d1c1dcb46e5806d1ec5ea32a8c12025-01-16T14:23:00ZengVeterinary WorldVeterinary World0972-89882231-09162025-01-0118110210910.14202/vetworld.2025.102-109Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomicsAthhar Manabi Diansyah0https://orcid.org/0009-0002-2338-8215Sanstoso Santoso1https://orcid.org/0000-0002-6873-8197Herdis Herdis2https://orcid.org/0000-0002-1586-2960Muhammad Yusuf3https://orcid.org/0000-0003-3421-4384Tri Puji Priyatno4https://orcid.org/0000-0002-2328-9607Tulus Maulana5https://orcid.org/0000-0002-5578-3992Abdul Latief Toleng6https://orcid.org/0009-0009-8351-9564Muhammad Ihsan Andi Dagong7https://orcid.org/0000-0001-5060-2614Syahruddin Said8https://orcid.org/0000-0001-8681-4985Hikmayani Iskandar9https://orcid.org/0000-0002-5786-5094Aeni Nurlatifah10https://orcid.org/0000-0003-3058-1195Puji Lestari11https://orcid.org/0000-0003-0615-4716Lukman Affandy12https://orcid.org/0000-0002-5153-3347Abdullah Baharun13https://orcid.org/0000-0002-3688-8844Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia; Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Indonesia. Jl. Perintis Kemerdekaan 10 Tamalanrea Makassar, South Sulawesi 90245, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia.Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Indonesia. Jl. Perintis Kemerdekaan 10 Tamalanrea Makassar, South Sulawesi 90245, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia.Research Center for Applied Zoology, National Research and Innovation Agency, Jl. Raya Bogor Km. 46, Cibinong, West Java 16911, Indonesia.Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Indonesia. Jl. Perintis Kemerdekaan 10 Tamalanrea Makassar, South Sulawesi 90245, Indonesia.Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Indonesia. Jl. Perintis Kemerdekaan 10 Tamalanrea Makassar, South Sulawesi 90245, Indonesia.Research Center for Applied Zoology, National Research and Innovation Agency, Jl. Raya Bogor Km. 46, Cibinong, West Java 16911, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia; Department of Nutrition and Feed Technology, Faculty of Animal Science, Gadjah Mada University, Depok, Sleman Regency, Special Region of Yogyakarta, Indonesia, 55281.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta, Bogor, West Java 16915, Indonesia.Department of Animal Science, Faculty of Agriculture, Djuanda University, Jl. Tol Ciawi No. 1, Ciawi, West Java 16720, Indonesia.Background and Aim: Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled bulls. Materials and Methods: Semen from five Bali-polled bulls was collected biweekly over five batches using artificial vaginas. Sperm kinematics were assessed using computer-assisted semen analysis (CASA). Fertility was evaluated through service per conception (S/C) in artificial insemination trials. Seminal plasma proteins were analyzed through liquid chromatography–tandem mass spectrometry (LC-MS/MS) and annotated using the UniProt database, PANTHER for gene ontology, and STRING database for protein interactions. Results: Post-thaw sperm kinematics showed satisfactory results, with a mean S/C of 1.52. Proteomic analysis identified 138 proteins, including six (TEX101, BSP1, PRSS55, BSP3, SPADH2, and TPPP2) linked to sperm motility. These proteins were involved in key biological processes such as sperm capacitation, motility regulation, and sperm-oocyte interaction. Conclusion: Sperm kinematics and seminal plasma proteomics provide insights into Bali-polled bull fertility. Identified proteins can serve as fertility biomarkers, aiding in superior local breed development and reproductive efficiency improvement.https://www.veterinaryworld.org/Vol.18/January-2025/12.pdfartificial inseminationbali-polled bullsfertility biomarkersseminal plasma proteomicssperm kinematics |
| spellingShingle | Athhar Manabi Diansyah Sanstoso Santoso Herdis Herdis Muhammad Yusuf Tri Puji Priyatno Tulus Maulana Abdul Latief Toleng Muhammad Ihsan Andi Dagong Syahruddin Said Hikmayani Iskandar Aeni Nurlatifah Puji Lestari Lukman Affandy Abdullah Baharun Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics Veterinary World artificial insemination bali-polled bulls fertility biomarkers seminal plasma proteomics sperm kinematics |
| title | Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics |
| title_full | Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics |
| title_fullStr | Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics |
| title_full_unstemmed | Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics |
| title_short | Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics |
| title_sort | identification of reproductive performance in bali polled bulls using computer assisted semen analysis and plasma seminal proteomics |
| topic | artificial insemination bali-polled bulls fertility biomarkers seminal plasma proteomics sperm kinematics |
| url | https://www.veterinaryworld.org/Vol.18/January-2025/12.pdf |
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