High Glucose Sensitizes Male and Female Rat Cardiomyocytes to Wnt/β-Catenin Signaling
Wnt/β-catenin signaling has been shown to regulate gene expressions in cardiomyocytes. However, it is not known if this effect is dependent on the sex of cells or the glucose level in the culture medium. In the present study, ventricular myocytes were prepared from male and female neonatal rats and...
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2024-12-01
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| author | Ruonan Gu Jerry Wang Julianne Morin Aizhu Lu Wenbin Liang |
| author_facet | Ruonan Gu Jerry Wang Julianne Morin Aizhu Lu Wenbin Liang |
| author_sort | Ruonan Gu |
| collection | DOAJ |
| description | Wnt/β-catenin signaling has been shown to regulate gene expressions in cardiomyocytes. However, it is not known if this effect is dependent on the sex of cells or the glucose level in the culture medium. In the present study, ventricular myocytes were prepared from male and female neonatal rats and maintained in either a glucose-rich (25 mM) medium or a low-glucose (3 mM), lipid-rich medium. Real-time quantitative PCR was used to measure changes in target genes (<i>Axin2</i>, <i>Scn5a,</i> and <i>Tbx3</i>) after treatment with 1, 3, or 10 µM of CHIR-99021, an activator of Wnt/β-catenin signaling. CHIR induced similar changes in <i>Axin2, Tbx3, and Scn5a</i> transcripts in male and female NRVMs in both media, suggesting the absence of sex difference. However, cells in a high-glucose medium showed greater increases in <i>Axin2</i> and <i>Tbx3</i> transcripts than cells in a low-glucose medium. In addition, a low concentration of CHIR (1 µM) reduced the <i>Scn5a</i> transcript in cells in a high-glucose medium but not in a low-glucose medium, suggesting an increased sensitivity to Wnt signaling by high glucose. A non-linear relationship was identified between <i>Axin2</i> transcript upregulation and <i>Scn5a</i> transcript downregulation in CIHR-treated NRVMs. These data suggest that high glucose sensitizes both male and female cardiomyocytes to Wnt/β-catenin signaling. |
| format | Article |
| id | doaj-art-efc60f6520354e2a93b0700a7ac65a96 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-12-01 |
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| series | Biomolecules |
| spelling | doaj-art-efc60f6520354e2a93b0700a7ac65a962024-12-27T14:14:01ZengMDPI AGBiomolecules2218-273X2024-12-011412163910.3390/biom14121639High Glucose Sensitizes Male and Female Rat Cardiomyocytes to Wnt/β-Catenin SignalingRuonan Gu0Jerry Wang1Julianne Morin2Aizhu Lu3Wenbin Liang4Cardiac Electrophysiology Laboratory, University of Ottawa Heart Institute, Ottawa, ON K1Y 4W7, CanadaCardiac Electrophysiology Laboratory, University of Ottawa Heart Institute, Ottawa, ON K1Y 4W7, CanadaCardiac Electrophysiology Laboratory, University of Ottawa Heart Institute, Ottawa, ON K1Y 4W7, CanadaCardiac Electrophysiology Laboratory, University of Ottawa Heart Institute, Ottawa, ON K1Y 4W7, CanadaCardiac Electrophysiology Laboratory, University of Ottawa Heart Institute, Ottawa, ON K1Y 4W7, CanadaWnt/β-catenin signaling has been shown to regulate gene expressions in cardiomyocytes. However, it is not known if this effect is dependent on the sex of cells or the glucose level in the culture medium. In the present study, ventricular myocytes were prepared from male and female neonatal rats and maintained in either a glucose-rich (25 mM) medium or a low-glucose (3 mM), lipid-rich medium. Real-time quantitative PCR was used to measure changes in target genes (<i>Axin2</i>, <i>Scn5a,</i> and <i>Tbx3</i>) after treatment with 1, 3, or 10 µM of CHIR-99021, an activator of Wnt/β-catenin signaling. CHIR induced similar changes in <i>Axin2, Tbx3, and Scn5a</i> transcripts in male and female NRVMs in both media, suggesting the absence of sex difference. However, cells in a high-glucose medium showed greater increases in <i>Axin2</i> and <i>Tbx3</i> transcripts than cells in a low-glucose medium. In addition, a low concentration of CHIR (1 µM) reduced the <i>Scn5a</i> transcript in cells in a high-glucose medium but not in a low-glucose medium, suggesting an increased sensitivity to Wnt signaling by high glucose. A non-linear relationship was identified between <i>Axin2</i> transcript upregulation and <i>Scn5a</i> transcript downregulation in CIHR-treated NRVMs. These data suggest that high glucose sensitizes both male and female cardiomyocytes to Wnt/β-catenin signaling.https://www.mdpi.com/2218-273X/14/12/1639Wnt signalingcardiac sodium channelScn5aglucose-rich medium |
| spellingShingle | Ruonan Gu Jerry Wang Julianne Morin Aizhu Lu Wenbin Liang High Glucose Sensitizes Male and Female Rat Cardiomyocytes to Wnt/β-Catenin Signaling Biomolecules Wnt signaling cardiac sodium channel Scn5a glucose-rich medium |
| title | High Glucose Sensitizes Male and Female Rat Cardiomyocytes to Wnt/β-Catenin Signaling |
| title_full | High Glucose Sensitizes Male and Female Rat Cardiomyocytes to Wnt/β-Catenin Signaling |
| title_fullStr | High Glucose Sensitizes Male and Female Rat Cardiomyocytes to Wnt/β-Catenin Signaling |
| title_full_unstemmed | High Glucose Sensitizes Male and Female Rat Cardiomyocytes to Wnt/β-Catenin Signaling |
| title_short | High Glucose Sensitizes Male and Female Rat Cardiomyocytes to Wnt/β-Catenin Signaling |
| title_sort | high glucose sensitizes male and female rat cardiomyocytes to wnt β catenin signaling |
| topic | Wnt signaling cardiac sodium channel Scn5a glucose-rich medium |
| url | https://www.mdpi.com/2218-273X/14/12/1639 |
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