Laxative Effect of Lactobacillus paracasei Glory LP16 on Loperamide‐Induced Constipation in Mice

ABSTRACT Constipation is a common gastrointestinal disease. In recent years, probiotic intervention has often been used to solve such problems. However, the results of existing studies on different intervention times and dose differences are not consistent. Therefore, it is necessary to conduct rese...

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Bibliographic Details
Main Authors: Weiwei Ma, Lian Lian, Lidong Guo, Yanan Wu, Lili Huang
Format: Article
Language:English
Published: Wiley 2025-08-01
Series:eFood
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Online Access:https://doi.org/10.1002/efd2.70068
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Summary:ABSTRACT Constipation is a common gastrointestinal disease. In recent years, probiotic intervention has often been used to solve such problems. However, the results of existing studies on different intervention times and dose differences are not consistent. Therefore, it is necessary to conduct research on specific strains. In this study, loperamide was used to establish the constipation model. By observing the basic fecal parameters, gastrointestinal characteristics, histological morphology, short‐chain fatty acids, and microbiota composition of constipated mice, the remission mechanisms of different doses of Lactobacillus paracasei Glory LP16 (LP16) on constipated mice were compared. It was found that different doses of LP16 could improve gastrointestinal peristalsis and neurotransmitter levels in constipated mice, reduce intestinal inflammation, significantly increase the concentration of short‐chain fatty acids, and improve the intestinal flora. Macrogenomics indicates that LP16 is mainly enriched in metabolic pathways such as amino acid biosynthesis and amino acid and nucleotide sugar metabolism. The expression of glycoside hydrolase in mice increased significantly. The results show that LP16 has laxative and antidiarrheal effects. Its laxative and antidiarrheal mechanisms may be related to its effects on constipation‐related neurotransmitters, improving intestinal flora, increasing fecal acetic acid content in constipated mice, and alleviating constipation‐induced inflammation.
ISSN:2666-3066