Virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed protein
The traditional nutritional and medical hemp (Cannabis sativa L.) seed protein were explored for the discovery and directional preparation of new xanthine oxidase inhibitory (XOI) peptides by structure-based virtual screening, compound synthesis, in vitro bioassay and proteolysis. Six subtypes of he...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2023.9250047 |
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| author | Xiaoni Huang Jiajun Liao Ping Shi Xiaodong Pei Chenghua Wang |
| author_facet | Xiaoni Huang Jiajun Liao Ping Shi Xiaodong Pei Chenghua Wang |
| author_sort | Xiaoni Huang |
| collection | DOAJ |
| description | The traditional nutritional and medical hemp (Cannabis sativa L.) seed protein were explored for the discovery and directional preparation of new xanthine oxidase inhibitory (XOI) peptides by structure-based virtual screening, compound synthesis, in vitro bioassay and proteolysis. Six subtypes of hemp seed edestin and albumin were in silico hydrolyzed by 29 proteases, and 192 encrypted bioactive peptides were screened out. Six peptides showed to be XOI peptides, of which four (about 67%) were released by elastase hydrolysis. The peptide DDNPRRFY displayed the highest XOI activity (IC50 = (2.10 ± 0.06) mg/mL), acting as a mixed inhibitor. The pancreatic elastase directionally prepared XOI hemp seed protein hydrolysates, from which 6 high-abundance XOI peptides encrypted 3 virtually-screened ones including the DDNPRRFY. The novel outstanding hemp seed protein-derived XOI peptides and their virtual screening and directed preparation methods provide a promising and applicable approach to conveniently and efficiently explore food-derived bioactive peptides. |
| format | Article |
| id | doaj-art-ef9b6f4ebcfa4f9ea33a94bdfa7242b1 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-ef9b6f4ebcfa4f9ea33a94bdfa7242b12025-01-10T06:57:02ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-11-011363652366010.26599/FSHW.2023.9250047Virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed proteinXiaoni Huang0Jiajun Liao1Ping Shi2Xiaodong Pei3Chenghua Wang4College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, ChinaCollege of Light Industry and Food Engineering, Guangxi University, Nanning 530004, ChinaCollege of Light Industry and Food Engineering, Guangxi University, Nanning 530004, ChinaCollege of Light Industry and Food Engineering, Guangxi University, Nanning 530004, ChinaCollege of Light Industry and Food Engineering, Guangxi University, Nanning 530004, ChinaThe traditional nutritional and medical hemp (Cannabis sativa L.) seed protein were explored for the discovery and directional preparation of new xanthine oxidase inhibitory (XOI) peptides by structure-based virtual screening, compound synthesis, in vitro bioassay and proteolysis. Six subtypes of hemp seed edestin and albumin were in silico hydrolyzed by 29 proteases, and 192 encrypted bioactive peptides were screened out. Six peptides showed to be XOI peptides, of which four (about 67%) were released by elastase hydrolysis. The peptide DDNPRRFY displayed the highest XOI activity (IC50 = (2.10 ± 0.06) mg/mL), acting as a mixed inhibitor. The pancreatic elastase directionally prepared XOI hemp seed protein hydrolysates, from which 6 high-abundance XOI peptides encrypted 3 virtually-screened ones including the DDNPRRFY. The novel outstanding hemp seed protein-derived XOI peptides and their virtual screening and directed preparation methods provide a promising and applicable approach to conveniently and efficiently explore food-derived bioactive peptides.https://www.sciopen.com/article/10.26599/FSHW.2023.9250047hemp seed proteinanti-goutbioactive peptidevirtual screeningproteolysis |
| spellingShingle | Xiaoni Huang Jiajun Liao Ping Shi Xiaodong Pei Chenghua Wang Virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed protein Food Science and Human Wellness hemp seed protein anti-gout bioactive peptide virtual screening proteolysis |
| title | Virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed protein |
| title_full | Virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed protein |
| title_fullStr | Virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed protein |
| title_full_unstemmed | Virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed protein |
| title_short | Virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed protein |
| title_sort | virtual screening and directional preparation of xanthine oxidase inhibitory peptides derived from hemp seed protein |
| topic | hemp seed protein anti-gout bioactive peptide virtual screening proteolysis |
| url | https://www.sciopen.com/article/10.26599/FSHW.2023.9250047 |
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