Development of ZnO-NPs reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility properties
Abstract This paper studied the possibility of fabricating a nano-composite based on chitosan incorporated with ZnO-NPs as a promising textile for wound dressing purposes. The nanofiber mat was obtained from dispersions of ZnO-NPs in chitosan-based solution blended with PVA (Cs/PVA/ZnO-NPs scaffold)...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-05-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-01669-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849310324827619328 |
|---|---|
| author | Parva Safari Eshagh Zakipour Rahimabadi Mohammad Reza Vaezi Aliasghar Behnamghader Reza Tahergorabi |
| author_facet | Parva Safari Eshagh Zakipour Rahimabadi Mohammad Reza Vaezi Aliasghar Behnamghader Reza Tahergorabi |
| author_sort | Parva Safari |
| collection | DOAJ |
| description | Abstract This paper studied the possibility of fabricating a nano-composite based on chitosan incorporated with ZnO-NPs as a promising textile for wound dressing purposes. The nanofiber mat was obtained from dispersions of ZnO-NPs in chitosan-based solution blended with PVA (Cs/PVA/ZnO-NPs scaffold). The extracted chitosan was characterized using FTIR, FE-SEM, XRD, and TGA analysis. The electrospinning optimization process was successfully done for Cs and PVA mixture and a good combination of polymers, solvent, and the ratios developed through an optimization process (10wt.% PVA and 1wt.% CS in AcAcetic 80%). The nanofibers had an average diameter below 200 nm, while the incorporation of ZnO-NPs decreased their average diameter below 150 nm. FTIR, FE-SEM, XRD analysis were used to evaluate the scaffold structure. The FE-SEM analysis proved the smooth and bead-free morphology of the fibers. Elemental analysis of the mat revealed a good distribution of ZnO-NPs along nanofibers. Cell culture studies with L929 mouse fibroblast cells revealed good viability of the cell on the Cs/PVA/ZnO-NPs scaffold. The nanoparticles improved capability of the mat for growth inhibition rate of bacterial colonies and also its wettability. The results also showed the nontoxicity of CS/PVA/ZnO-NPs composite and its considerable potential for future application in wound dressing. |
| format | Article |
| id | doaj-art-ef3d1f3e7bb7407d9b6f65feb8981d19 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ef3d1f3e7bb7407d9b6f65feb8981d192025-08-20T03:53:46ZengNature PortfolioScientific Reports2045-23222025-05-0115111710.1038/s41598-025-01669-wDevelopment of ZnO-NPs reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility propertiesParva Safari0Eshagh Zakipour Rahimabadi1Mohammad Reza Vaezi2Aliasghar Behnamghader3Reza Tahergorabi4Fisheries Department, Faculty of Natural Resources, University of GuilanFisheries Department, Faculty of Natural Resources, University of GuilanResearch Department of Nanotechnology and Advanced Materials, , Materials and Energy Research CenterResearch Department of Nanotechnology and Advanced Materials, , Materials and Energy Research CenterFood and Nutritional Sciences Program, North Carolina Agricultural and Technical State UniversityAbstract This paper studied the possibility of fabricating a nano-composite based on chitosan incorporated with ZnO-NPs as a promising textile for wound dressing purposes. The nanofiber mat was obtained from dispersions of ZnO-NPs in chitosan-based solution blended with PVA (Cs/PVA/ZnO-NPs scaffold). The extracted chitosan was characterized using FTIR, FE-SEM, XRD, and TGA analysis. The electrospinning optimization process was successfully done for Cs and PVA mixture and a good combination of polymers, solvent, and the ratios developed through an optimization process (10wt.% PVA and 1wt.% CS in AcAcetic 80%). The nanofibers had an average diameter below 200 nm, while the incorporation of ZnO-NPs decreased their average diameter below 150 nm. FTIR, FE-SEM, XRD analysis were used to evaluate the scaffold structure. The FE-SEM analysis proved the smooth and bead-free morphology of the fibers. Elemental analysis of the mat revealed a good distribution of ZnO-NPs along nanofibers. Cell culture studies with L929 mouse fibroblast cells revealed good viability of the cell on the Cs/PVA/ZnO-NPs scaffold. The nanoparticles improved capability of the mat for growth inhibition rate of bacterial colonies and also its wettability. The results also showed the nontoxicity of CS/PVA/ZnO-NPs composite and its considerable potential for future application in wound dressing.https://doi.org/10.1038/s41598-025-01669-wChitosanElectrospinningNanofiberOptimizationWound dressingZnO-NPs |
| spellingShingle | Parva Safari Eshagh Zakipour Rahimabadi Mohammad Reza Vaezi Aliasghar Behnamghader Reza Tahergorabi Development of ZnO-NPs reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility properties Scientific Reports Chitosan Electrospinning Nanofiber Optimization Wound dressing ZnO-NPs |
| title | Development of ZnO-NPs reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility properties |
| title_full | Development of ZnO-NPs reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility properties |
| title_fullStr | Development of ZnO-NPs reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility properties |
| title_full_unstemmed | Development of ZnO-NPs reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility properties |
| title_short | Development of ZnO-NPs reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility properties |
| title_sort | development of zno nps reinforced chitosan nanofiber mats with improved antibacterial and biocompatibility properties |
| topic | Chitosan Electrospinning Nanofiber Optimization Wound dressing ZnO-NPs |
| url | https://doi.org/10.1038/s41598-025-01669-w |
| work_keys_str_mv | AT parvasafari developmentofznonpsreinforcedchitosannanofibermatswithimprovedantibacterialandbiocompatibilityproperties AT eshaghzakipourrahimabadi developmentofznonpsreinforcedchitosannanofibermatswithimprovedantibacterialandbiocompatibilityproperties AT mohammadrezavaezi developmentofznonpsreinforcedchitosannanofibermatswithimprovedantibacterialandbiocompatibilityproperties AT aliasgharbehnamghader developmentofznonpsreinforcedchitosannanofibermatswithimprovedantibacterialandbiocompatibilityproperties AT rezatahergorabi developmentofznonpsreinforcedchitosannanofibermatswithimprovedantibacterialandbiocompatibilityproperties |