Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meat
Klebsiella spp. present in the food chain have been of much interest during the last few decades due to their implication in the development of antimicrobial resistance. We determined the prevalence of Klebsiella spp. (Klebsiella pneumoniae and Klebsiella oxytoca) in retail poultry meat, along with...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025001288 |
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| author | Fatema Yeasmin Tanni Md. Shahidur Rahman Chowdhury Hemayet Hossain Md. Atik Faysal Md. Anisur Rahman Ahsan Al Emon Mashuka Nahida Asha Md. Mukter Hossain Md. Mahfujur Rahman |
| author_facet | Fatema Yeasmin Tanni Md. Shahidur Rahman Chowdhury Hemayet Hossain Md. Atik Faysal Md. Anisur Rahman Ahsan Al Emon Mashuka Nahida Asha Md. Mukter Hossain Md. Mahfujur Rahman |
| author_sort | Fatema Yeasmin Tanni |
| collection | DOAJ |
| description | Klebsiella spp. present in the food chain have been of much interest during the last few decades due to their implication in the development of antimicrobial resistance. We determined the prevalence of Klebsiella spp. (Klebsiella pneumoniae and Klebsiella oxytoca) in retail poultry meat, along with their resistance profile against antimicrobials. For the detection of the Klebsiella spp. a total of 285 raw meat samples of retailed poultry were collected and cultured. All positive cultures were screened for Klebsiella spp. by PCR to confirm the identity. Their antimicrobial susceptibility pattern was performed using the disk diffusion technique, whereas the ESBL-coding genes were detected using the multiplex PCR technique. Out of 285 samples, 139 (48.77 %) raw meat samples had Klebsiella spp. While out of 139 identified positive isolates, 99 (34.74 %) isolates were K. pneumoniae, and 13 (4.56 %) were K. oxytoca. Phenotypically, most of the strains were fully (100 %) resistant to ampicillin, amoxicillin, cefuroxime, ceftazidime, and nalidixic acid. Of the β-Lactamase genes that were detected through mPCR, blaSHV was the dominant gene among the strains K. pneumoniae and K. oxytoca isolates, with percentages of 86.87 % and 76.92 %, respectively. Besides, isolates of K. pneumoniae harbored 95.96 % (95 % CI: 89.98–98.89) of the tetA gene, whereas the K. oxytoca isolates harbored only 92.31 % (95 % CI: 63.97–99.81) of the tetA gene. Most of the K. pneumoniae and K. oxytoca isolates were found to harbor the streptomycin-resistant gene, strA. These results highlighted significant carriage of multidrug-resistant (MDR) Klebsiella spp. in retail poultry meat, which insinuates that there is a need for a strategic plan in place to curb the dissemination of these multi drug resistant pathogens along the food chain. |
| format | Article |
| id | doaj-art-ef2643a6b9a04c4fa8e5f9ccb5f4fe7e |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-ef2643a6b9a04c4fa8e5f9ccb5f4fe7e2025-01-17T04:51:59ZengElsevierHeliyon2405-84402025-01-01111e41748Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meatFatema Yeasmin Tanni0Md. Shahidur Rahman Chowdhury1Hemayet Hossain2Md. Atik Faysal3Md. Anisur Rahman4Ahsan Al Emon5Mashuka Nahida Asha6Md. Mukter Hossain7Md. Mahfujur Rahman8Department of Medicine, Sylhet Agricultural University, Sylhet, 3100, Bangladesh; Department of Biochemistry, University of Nebraska-Lincoln, Beadle Center, 1901 Vine St, Lincoln, NE, 68503, United StatesDepartment of Medicine, Sylhet Agricultural University, Sylhet, 3100, BangladeshDepartment of Anatomy and Histology, Sylhet Agricultural University, Sylhet, 3100, BangladeshDepartment of Medicine, Sylhet Agricultural University, Sylhet, 3100, Bangladesh; School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, NE, 68583, United StatesDepartment of Medicine, Sylhet Agricultural University, Sylhet, 3100, BangladeshDepartment of Medicine, Sylhet Agricultural University, Sylhet, 3100, BangladeshDepartment of Medicine, Sylhet Agricultural University, Sylhet, 3100, BangladeshDepartment of Medicine, Sylhet Agricultural University, Sylhet, 3100, BangladeshDepartment of Medicine, Sylhet Agricultural University, Sylhet, 3100, Bangladesh; Corresponding author.Klebsiella spp. present in the food chain have been of much interest during the last few decades due to their implication in the development of antimicrobial resistance. We determined the prevalence of Klebsiella spp. (Klebsiella pneumoniae and Klebsiella oxytoca) in retail poultry meat, along with their resistance profile against antimicrobials. For the detection of the Klebsiella spp. a total of 285 raw meat samples of retailed poultry were collected and cultured. All positive cultures were screened for Klebsiella spp. by PCR to confirm the identity. Their antimicrobial susceptibility pattern was performed using the disk diffusion technique, whereas the ESBL-coding genes were detected using the multiplex PCR technique. Out of 285 samples, 139 (48.77 %) raw meat samples had Klebsiella spp. While out of 139 identified positive isolates, 99 (34.74 %) isolates were K. pneumoniae, and 13 (4.56 %) were K. oxytoca. Phenotypically, most of the strains were fully (100 %) resistant to ampicillin, amoxicillin, cefuroxime, ceftazidime, and nalidixic acid. Of the β-Lactamase genes that were detected through mPCR, blaSHV was the dominant gene among the strains K. pneumoniae and K. oxytoca isolates, with percentages of 86.87 % and 76.92 %, respectively. Besides, isolates of K. pneumoniae harbored 95.96 % (95 % CI: 89.98–98.89) of the tetA gene, whereas the K. oxytoca isolates harbored only 92.31 % (95 % CI: 63.97–99.81) of the tetA gene. Most of the K. pneumoniae and K. oxytoca isolates were found to harbor the streptomycin-resistant gene, strA. These results highlighted significant carriage of multidrug-resistant (MDR) Klebsiella spp. in retail poultry meat, which insinuates that there is a need for a strategic plan in place to curb the dissemination of these multi drug resistant pathogens along the food chain.http://www.sciencedirect.com/science/article/pii/S2405844025001288PrevalenceAntimicrobial resistanceESBLKlebsiella spp.Poultry meat |
| spellingShingle | Fatema Yeasmin Tanni Md. Shahidur Rahman Chowdhury Hemayet Hossain Md. Atik Faysal Md. Anisur Rahman Ahsan Al Emon Mashuka Nahida Asha Md. Mukter Hossain Md. Mahfujur Rahman Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meat Heliyon Prevalence Antimicrobial resistance ESBL Klebsiella spp. Poultry meat |
| title | Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meat |
| title_full | Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meat |
| title_fullStr | Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meat |
| title_full_unstemmed | Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meat |
| title_short | Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meat |
| title_sort | prevalence and antimicrobial resistance of extended spectrum beta lactamase esbl producing klebsiella spp in poultry meat |
| topic | Prevalence Antimicrobial resistance ESBL Klebsiella spp. Poultry meat |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025001288 |
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