Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces
Abstract Tuberculosis (TB) is the top cause of death from a single infectious pathogen after COVID-19. Despite molecular diagnostic advances, two-thirds of the 10 million annual TB cases are still diagnosed using direct smear microscopy which has ~50% sensitivity. To increase the analytical performa...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2024-09-01
|
| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06829-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846112958974787584 |
|---|---|
| author | Xuesong Jiang Bonolo S. P. Mathekga Digvijay Singh Devin Coon Anjana Sinha Derek Armstrong Soumyadipta Acharya Hai-Quan Mao Yukari C. Manabe |
| author_facet | Xuesong Jiang Bonolo S. P. Mathekga Digvijay Singh Devin Coon Anjana Sinha Derek Armstrong Soumyadipta Acharya Hai-Quan Mao Yukari C. Manabe |
| author_sort | Xuesong Jiang |
| collection | DOAJ |
| description | Abstract Tuberculosis (TB) is the top cause of death from a single infectious pathogen after COVID-19. Despite molecular diagnostic advances, two-thirds of the 10 million annual TB cases are still diagnosed using direct smear microscopy which has ~50% sensitivity. To increase the analytical performance of smear microscopy, we developed and characterized a novel polymer (Polydiallyldimethylammonium chloride [PDADMAC]) engraftment on inexpensive polystyrene (PS) specifically functionalized for mycobacterial capture. Engraftment is achieved via UV photopolymerization of DADMAC monomer on plasma-activated PS. The platform was tested on sputum from presumptive TB cases in Kampala, Uganda (n = 50), with an increased overall sensitivity of 81.8% (27/33) vs. fluorescent smear microscopy 57% (19/33) compared to a molecular (Cepheid GeneXpert MTB/RIF) gold standard. Frugal smear diagnostic innovation that is rapid and does not require dedicated instrumentation may offer an important solution to bridge the TB diagnostic gap. Graphical Abstract |
| format | Article |
| id | doaj-art-eef7d32b7f814a7d9b74afd873befc61 |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-eef7d32b7f814a7d9b74afd873befc612024-12-22T12:10:50ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382024-09-0135111010.1007/s10856-024-06829-4Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfacesXuesong Jiang0Bonolo S. P. Mathekga1Digvijay Singh2Devin Coon3Anjana Sinha4Derek Armstrong5Soumyadipta Acharya6Hai-Quan Mao7Yukari C. Manabe8Department of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins UniversityCenter for Bioengineering Innovation and Design, Johns Hopkins UniversityCenter for Bioengineering Innovation and Design, Johns Hopkins UniversityDepartments of Plastic and Reconstructive Surgery, School of Medicine, Johns Hopkins UniversityCenter for Bioengineering Innovation and Design, Johns Hopkins UniversityDivision of Infectious Diseases, Department of Medicine, Johns Hopkins University School of MedicineCenter for Bioengineering Innovation and Design, Johns Hopkins UniversityDepartment of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins UniversityDivision of Infectious Diseases, Department of Medicine, Johns Hopkins University School of MedicineAbstract Tuberculosis (TB) is the top cause of death from a single infectious pathogen after COVID-19. Despite molecular diagnostic advances, two-thirds of the 10 million annual TB cases are still diagnosed using direct smear microscopy which has ~50% sensitivity. To increase the analytical performance of smear microscopy, we developed and characterized a novel polymer (Polydiallyldimethylammonium chloride [PDADMAC]) engraftment on inexpensive polystyrene (PS) specifically functionalized for mycobacterial capture. Engraftment is achieved via UV photopolymerization of DADMAC monomer on plasma-activated PS. The platform was tested on sputum from presumptive TB cases in Kampala, Uganda (n = 50), with an increased overall sensitivity of 81.8% (27/33) vs. fluorescent smear microscopy 57% (19/33) compared to a molecular (Cepheid GeneXpert MTB/RIF) gold standard. Frugal smear diagnostic innovation that is rapid and does not require dedicated instrumentation may offer an important solution to bridge the TB diagnostic gap. Graphical Abstracthttps://doi.org/10.1007/s10856-024-06829-4 |
| spellingShingle | Xuesong Jiang Bonolo S. P. Mathekga Digvijay Singh Devin Coon Anjana Sinha Derek Armstrong Soumyadipta Acharya Hai-Quan Mao Yukari C. Manabe Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces Journal of Materials Science: Materials in Medicine |
| title | Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces |
| title_full | Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces |
| title_fullStr | Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces |
| title_full_unstemmed | Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces |
| title_short | Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces |
| title_sort | selective mycobacterial capture with ultraviolet polymerized poly dimethyldiallyl chloride functionalized surfaces |
| url | https://doi.org/10.1007/s10856-024-06829-4 |
| work_keys_str_mv | AT xuesongjiang selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces AT bonolospmathekga selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces AT digvijaysingh selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces AT devincoon selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces AT anjanasinha selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces AT derekarmstrong selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces AT soumyadiptaacharya selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces AT haiquanmao selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces AT yukaricmanabe selectivemycobacterialcapturewithultravioletpolymerizedpolydimethyldiallylchloridefunctionalizedsurfaces |