Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods
Abstract The procedure of anterior cruciate ligament (ACL) allograft preparation can be divided into fresh-frozen method (FF-allograft) or freeze-dried method (FD-allograft). This study aims to biomechanically and histologically compare the graft to bone tunnel integration between the two allografts...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-11-01
|
| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-024-05191-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846171782025838592 |
|---|---|
| author | Xiaoke Shang Jianzhong Hu Jin Qu Peng Wen Jian Li Qi Li Jun Zheng |
| author_facet | Xiaoke Shang Jianzhong Hu Jin Qu Peng Wen Jian Li Qi Li Jun Zheng |
| author_sort | Xiaoke Shang |
| collection | DOAJ |
| description | Abstract The procedure of anterior cruciate ligament (ACL) allograft preparation can be divided into fresh-frozen method (FF-allograft) or freeze-dried method (FD-allograft). This study aims to biomechanically and histologically compare the graft to bone tunnel integration between the two allografts. In-vitro results indicated that FF-allograft and FD-allograft showed excellent biocompatibility and biomechanics, while FD-allograft showed a denser collagen fiber arrangement than FF-allograft and autograft. Then, in-vivo preformation of the FF-allograft, FD-allograft, and autograft on bone tunnel integration was evaluated via a canine ACL reconstruction model. In-vivo results indicated that no signs of infection or osteoarthritis were shown in the femur-graft-tibia complexes, but more vascularity and synovitis formed around the implanted FF-allograft. Micro-computed tomography showed that peri-graft bone in the FF-allograft group was significantly increased and remodeled compared with the FD-allograft group; Histologically, the FF-allograft group exhibited similar graft-bone tunnel healing to the FD-allograft group. Tartrate-resistant acid phosphatase (TRAP) staining showed significantly more osteoclasts presented in the FD-allograft group compared to the FF-allograft group. Meanwhile, a significantly higher failure load was shown in the FF-allograft group when compared with the FD-allograft group (P < 0.05). In conclusion, the FF-allograft integrated more firmly into the bone tunnel than the FD-allograft when used in ACL reconstruction. |
| format | Article |
| id | doaj-art-eef437cd6fbb4d849396f27c3a64d5a0 |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-eef437cd6fbb4d849396f27c3a64d5a02024-11-10T12:34:51ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-11-0119111510.1186/s13018-024-05191-zAllograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methodsXiaoke Shang0Jianzhong Hu1Jin Qu2Peng Wen3Jian Li4Qi Li5Jun Zheng6Department of Orthopedic Surgery, People’s Hospital of Ningxia Hui Autonomous RegionDepartment of Spinal Surgery, Xiangya Hospital, Central South UniversityDepartment of Sports Medicine, Xiangya Hospital, Central South UniversityDepartment of Orthopedic Surgery, People’s Hospital of Ningxia Hui Autonomous RegionDepartment of Orthopedic Surgery, West China Hospital of Sichuan University, Sichuan UniversityDepartment of Orthopedic Surgery, West China Hospital of Sichuan University, Sichuan UniversityDepartment of Orthopedic Surgery, People’s Hospital of Ningxia Hui Autonomous RegionAbstract The procedure of anterior cruciate ligament (ACL) allograft preparation can be divided into fresh-frozen method (FF-allograft) or freeze-dried method (FD-allograft). This study aims to biomechanically and histologically compare the graft to bone tunnel integration between the two allografts. In-vitro results indicated that FF-allograft and FD-allograft showed excellent biocompatibility and biomechanics, while FD-allograft showed a denser collagen fiber arrangement than FF-allograft and autograft. Then, in-vivo preformation of the FF-allograft, FD-allograft, and autograft on bone tunnel integration was evaluated via a canine ACL reconstruction model. In-vivo results indicated that no signs of infection or osteoarthritis were shown in the femur-graft-tibia complexes, but more vascularity and synovitis formed around the implanted FF-allograft. Micro-computed tomography showed that peri-graft bone in the FF-allograft group was significantly increased and remodeled compared with the FD-allograft group; Histologically, the FF-allograft group exhibited similar graft-bone tunnel healing to the FD-allograft group. Tartrate-resistant acid phosphatase (TRAP) staining showed significantly more osteoclasts presented in the FD-allograft group compared to the FF-allograft group. Meanwhile, a significantly higher failure load was shown in the FF-allograft group when compared with the FD-allograft group (P < 0.05). In conclusion, the FF-allograft integrated more firmly into the bone tunnel than the FD-allograft when used in ACL reconstruction.https://doi.org/10.1186/s13018-024-05191-zAnterior cruciate ligamentAutograftAllograftGraft storageTendon-bone healing |
| spellingShingle | Xiaoke Shang Jianzhong Hu Jin Qu Peng Wen Jian Li Qi Li Jun Zheng Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods Journal of Orthopaedic Surgery and Research Anterior cruciate ligament Autograft Allograft Graft storage Tendon-bone healing |
| title | Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods |
| title_full | Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods |
| title_fullStr | Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods |
| title_full_unstemmed | Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods |
| title_short | Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods |
| title_sort | allograft to bone tunnel integration in a canine anterior cruciate ligament reconstruction model a comparison study of allograft preparation methods |
| topic | Anterior cruciate ligament Autograft Allograft Graft storage Tendon-bone healing |
| url | https://doi.org/10.1186/s13018-024-05191-z |
| work_keys_str_mv | AT xiaokeshang allografttobonetunnelintegrationinacanineanteriorcruciateligamentreconstructionmodelacomparisonstudyofallograftpreparationmethods AT jianzhonghu allografttobonetunnelintegrationinacanineanteriorcruciateligamentreconstructionmodelacomparisonstudyofallograftpreparationmethods AT jinqu allografttobonetunnelintegrationinacanineanteriorcruciateligamentreconstructionmodelacomparisonstudyofallograftpreparationmethods AT pengwen allografttobonetunnelintegrationinacanineanteriorcruciateligamentreconstructionmodelacomparisonstudyofallograftpreparationmethods AT jianli allografttobonetunnelintegrationinacanineanteriorcruciateligamentreconstructionmodelacomparisonstudyofallograftpreparationmethods AT qili allografttobonetunnelintegrationinacanineanteriorcruciateligamentreconstructionmodelacomparisonstudyofallograftpreparationmethods AT junzheng allografttobonetunnelintegrationinacanineanteriorcruciateligamentreconstructionmodelacomparisonstudyofallograftpreparationmethods |