Geometrically engineered organoid units and their assembly for pre-construction of organ structures
Regenerative medicine is moving from the nascent to the transitional stage as researchers are actively engaged in creating mini-organs from pluripotent stem cells to construct artificial models of physiological and pathological conditions. Currently, mini-organs can express higher-order functions, b...
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2024-12-01
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| Series: | APL Bioengineering |
| Online Access: | http://dx.doi.org/10.1063/5.0222866 |
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| author | Ayaka Kadotani Gen Hayase Daisuke Yoshino |
| author_facet | Ayaka Kadotani Gen Hayase Daisuke Yoshino |
| author_sort | Ayaka Kadotani |
| collection | DOAJ |
| description | Regenerative medicine is moving from the nascent to the transitional stage as researchers are actively engaged in creating mini-organs from pluripotent stem cells to construct artificial models of physiological and pathological conditions. Currently, mini-organs can express higher-order functions, but their size is limited to the order of a few millimeters. Therefore, one of the ultimate goals of regenerative medicine, “organ replication and transplantation with organoid,” remains a major obstacle. Three-dimensional (3D) bioprinting technology is expected to be an innovative breakthrough in this field, but various issues have been raised, such as cell damage, versatility of bioink, and printing time. In this study, we established a method for fabricating, connecting, and assembling organoid units of various shapes independent of cell type, extracellular matrix, and adhesive composition (unit construction method). We also fabricated kidney tissue-like structures using three types of parenchymal and interstitial cells that compose the human kidney and obtained findings suggesting the possibility of crosstalk between the units. This study mainly focuses on methods for reproducing the structure of organs, and there are still issues to be addressed in terms of the expression of their higher-order functions. We anticipate that engineering innovation based on this technique will bring us closer to the realization of highly efficient and rapid fabrication of full-scale organoids that can withstand organ transplantation. |
| format | Article |
| id | doaj-art-eef3c67afc894f68a8df9b8376c025c5 |
| institution | Kabale University |
| issn | 2473-2877 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | APL Bioengineering |
| spelling | doaj-art-eef3c67afc894f68a8df9b8376c025c52025-01-02T17:08:49ZengAIP Publishing LLCAPL Bioengineering2473-28772024-12-0184046112046112-1210.1063/5.0222866Geometrically engineered organoid units and their assembly for pre-construction of organ structuresAyaka Kadotani0Gen Hayase1Daisuke Yoshino2 Department of Biomedical Engineering, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan Research Center for Electronic and Optical Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan Department of Biomedical Engineering, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, JapanRegenerative medicine is moving from the nascent to the transitional stage as researchers are actively engaged in creating mini-organs from pluripotent stem cells to construct artificial models of physiological and pathological conditions. Currently, mini-organs can express higher-order functions, but their size is limited to the order of a few millimeters. Therefore, one of the ultimate goals of regenerative medicine, “organ replication and transplantation with organoid,” remains a major obstacle. Three-dimensional (3D) bioprinting technology is expected to be an innovative breakthrough in this field, but various issues have been raised, such as cell damage, versatility of bioink, and printing time. In this study, we established a method for fabricating, connecting, and assembling organoid units of various shapes independent of cell type, extracellular matrix, and adhesive composition (unit construction method). We also fabricated kidney tissue-like structures using three types of parenchymal and interstitial cells that compose the human kidney and obtained findings suggesting the possibility of crosstalk between the units. This study mainly focuses on methods for reproducing the structure of organs, and there are still issues to be addressed in terms of the expression of their higher-order functions. We anticipate that engineering innovation based on this technique will bring us closer to the realization of highly efficient and rapid fabrication of full-scale organoids that can withstand organ transplantation.http://dx.doi.org/10.1063/5.0222866 |
| spellingShingle | Ayaka Kadotani Gen Hayase Daisuke Yoshino Geometrically engineered organoid units and their assembly for pre-construction of organ structures APL Bioengineering |
| title | Geometrically engineered organoid units and their assembly for pre-construction of organ structures |
| title_full | Geometrically engineered organoid units and their assembly for pre-construction of organ structures |
| title_fullStr | Geometrically engineered organoid units and their assembly for pre-construction of organ structures |
| title_full_unstemmed | Geometrically engineered organoid units and their assembly for pre-construction of organ structures |
| title_short | Geometrically engineered organoid units and their assembly for pre-construction of organ structures |
| title_sort | geometrically engineered organoid units and their assembly for pre construction of organ structures |
| url | http://dx.doi.org/10.1063/5.0222866 |
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