Effects of selenium nanoparticles on cadmium uptake, selenium transformation, and metabolomics in Agaricus Blazei murill

Abstract Selenium nanoparticles (Se NPs) primarily reduce cadmium content in plants. However, the mechanism by which Se NPs affect the Cd content in edible mushrooms is unknown. This study addressed this by exogenously adding Se NPs and Cd(NO3)2 to the cultivated substrates of Agaricus blazei Murill...

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Main Authors: Nana Luo, Baojiao Wang, Rui Yu, Guoshuang Chen
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-97260-4
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author Nana Luo
Baojiao Wang
Rui Yu
Guoshuang Chen
author_facet Nana Luo
Baojiao Wang
Rui Yu
Guoshuang Chen
author_sort Nana Luo
collection DOAJ
description Abstract Selenium nanoparticles (Se NPs) primarily reduce cadmium content in plants. However, the mechanism by which Se NPs affect the Cd content in edible mushrooms is unknown. This study addressed this by exogenously adding Se NPs and Cd(NO3)2 to the cultivated substrates of Agaricus blazei Murill (AbM). The results indicated that Cd was easily enriched in the AbM fruiting body, while adding Se NPs significantly decreased the Cd content of AbM by 48.7–69.4%. The percentages of Cd in cell wall fraction (Fw), soluble fraction (Fs), and organelle fraction (Fo) reached 82.9–95.8%, 3.6–15.7%, and 0.6–3.2%, respectively. Se NPs reduced Cd toxicity to AbM by alleviating oxidative stress. Se existed in the fruiting body as selenocysteine (SeCys) and selenomethionine (SeMet). Se NPs could significantly affect the type and content of metabolites in the AbM fruiting body. In conclusion, applying Se NPs to cultivated substrates may mitigate Cd toxicity by reducing Cd uptake into the fruiting body, altering the subcellular distribution of Cd, alleviating oxidative stress, and altering the type, content, and biometabolic pathways of metabolites.
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spelling doaj-art-eed49e88c9b64aaa933a004429463c992025-08-20T03:45:35ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-97260-4Effects of selenium nanoparticles on cadmium uptake, selenium transformation, and metabolomics in Agaricus Blazei murillNana Luo0Baojiao Wang1Rui Yu2Guoshuang Chen3State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesCollege of Agriculture, Yanbian UniversityState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesAbstract Selenium nanoparticles (Se NPs) primarily reduce cadmium content in plants. However, the mechanism by which Se NPs affect the Cd content in edible mushrooms is unknown. This study addressed this by exogenously adding Se NPs and Cd(NO3)2 to the cultivated substrates of Agaricus blazei Murill (AbM). The results indicated that Cd was easily enriched in the AbM fruiting body, while adding Se NPs significantly decreased the Cd content of AbM by 48.7–69.4%. The percentages of Cd in cell wall fraction (Fw), soluble fraction (Fs), and organelle fraction (Fo) reached 82.9–95.8%, 3.6–15.7%, and 0.6–3.2%, respectively. Se NPs reduced Cd toxicity to AbM by alleviating oxidative stress. Se existed in the fruiting body as selenocysteine (SeCys) and selenomethionine (SeMet). Se NPs could significantly affect the type and content of metabolites in the AbM fruiting body. In conclusion, applying Se NPs to cultivated substrates may mitigate Cd toxicity by reducing Cd uptake into the fruiting body, altering the subcellular distribution of Cd, alleviating oxidative stress, and altering the type, content, and biometabolic pathways of metabolites.https://doi.org/10.1038/s41598-025-97260-4Edible mushroomsSelenium nanoparticlesCadmiumSubcellular distributionSelenium chemical formsNon-targeted metabolomics
spellingShingle Nana Luo
Baojiao Wang
Rui Yu
Guoshuang Chen
Effects of selenium nanoparticles on cadmium uptake, selenium transformation, and metabolomics in Agaricus Blazei murill
Scientific Reports
Edible mushrooms
Selenium nanoparticles
Cadmium
Subcellular distribution
Selenium chemical forms
Non-targeted metabolomics
title Effects of selenium nanoparticles on cadmium uptake, selenium transformation, and metabolomics in Agaricus Blazei murill
title_full Effects of selenium nanoparticles on cadmium uptake, selenium transformation, and metabolomics in Agaricus Blazei murill
title_fullStr Effects of selenium nanoparticles on cadmium uptake, selenium transformation, and metabolomics in Agaricus Blazei murill
title_full_unstemmed Effects of selenium nanoparticles on cadmium uptake, selenium transformation, and metabolomics in Agaricus Blazei murill
title_short Effects of selenium nanoparticles on cadmium uptake, selenium transformation, and metabolomics in Agaricus Blazei murill
title_sort effects of selenium nanoparticles on cadmium uptake selenium transformation and metabolomics in agaricus blazei murill
topic Edible mushrooms
Selenium nanoparticles
Cadmium
Subcellular distribution
Selenium chemical forms
Non-targeted metabolomics
url https://doi.org/10.1038/s41598-025-97260-4
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AT baojiaowang effectsofseleniumnanoparticlesoncadmiumuptakeseleniumtransformationandmetabolomicsinagaricusblazeimurill
AT ruiyu effectsofseleniumnanoparticlesoncadmiumuptakeseleniumtransformationandmetabolomicsinagaricusblazeimurill
AT guoshuangchen effectsofseleniumnanoparticlesoncadmiumuptakeseleniumtransformationandmetabolomicsinagaricusblazeimurill