Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method
This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and ex...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga, Faculty of Veterinary Medicine
2022-10-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/38246 |
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| author | Jola Rahmahani Maha Kirana Fedik Abdul Rantam Suwarno Suwarno |
| author_facet | Jola Rahmahani Maha Kirana Fedik Abdul Rantam Suwarno Suwarno |
| author_sort | Jola Rahmahani |
| collection | DOAJ |
| description | This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B ( 5'-TAGTGATGMCGSGACATCAT-3' ) with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus. |
| format | Article |
| id | doaj-art-eed3dacfb0d64e9bad5943bff70b5b58 |
| institution | Kabale University |
| issn | 2615-7497 2581-012X |
| language | English |
| publishDate | 2022-10-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj-art-eed3dacfb0d64e9bad5943bff70b5b582025-01-02T19:41:36ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2022-10-015214514810.20473/jmv.vol5.iss2.2022.145-14836316Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction MethodJola Rahmahani0https://orcid.org/0000-0001-9103-3200Maha Kirana1Fedik Abdul Rantam2https://orcid.org/0000-0002-1047-2410Suwarno Suwarno3https://orcid.org/0000-0002-4997-5242Laboratorium Virologi dan Imunologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya, IndonesiaLaboratorium Vaksinologi dan Imunologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya, IndonesiaLaboratorium Virologi dan Imunologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya, IndonesiaLaboratorium Virologi dan Imunologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya, IndonesiaThis study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B ( 5'-TAGTGATGMCGSGACATCAT-3' ) with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus.https://e-journal.unair.ac.id/JMV/article/view/38246inclusion bodies hepatitispolymerase chain reactionbroiler |
| spellingShingle | Jola Rahmahani Maha Kirana Fedik Abdul Rantam Suwarno Suwarno Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method Jurnal Medik Veteriner inclusion bodies hepatitis polymerase chain reaction broiler |
| title | Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method |
| title_full | Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method |
| title_fullStr | Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method |
| title_full_unstemmed | Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method |
| title_short | Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method |
| title_sort | diagnosis confirmation of inclusion bodies hepatitis on broiler chicken using polymerase chain reaction method |
| topic | inclusion bodies hepatitis polymerase chain reaction broiler |
| url | https://e-journal.unair.ac.id/JMV/article/view/38246 |
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