The investigation of israil acute bee paralysis virus, sacbrood virus, kashmir bee virus and chronic bee paralysis virus in honeybees (apis mellifera)
<b>Aim: </b>This study aims to investigate the presence and prevalence of Israel acute bee paralysis virus (IAPV), Sacbrood virus (SBV), Kashmir bee virus (KBV) and Chronic bee paralysis virus (CBPV) by Reverz Transcriptase-Polymerase Chain Reaction (RT-PCR) method in apiaries around Bur...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2020-06-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1284 |
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| Summary: | <b>Aim: </b>This study aims to investigate the presence and prevalence of Israel acute
bee paralysis virus (IAPV), Sacbrood virus (SBV), Kashmir bee virus (KBV) and
Chronic bee paralysis virus (CBPV) by Reverz Transcriptase-Polymerase Chain
Reaction (RT-PCR) method in apiaries around Burdur region.<p>
<b>Materials and Methods:</b> During the study, 30 beehives from 15 apiaries were
selected randomly and sampling procedures were carried out. 30 adult bees
were collected from each of the sampled hives to form a pool. After the samples
were homogenized, total nucleic acid extraction was performed and then
viral nucleic acid was searched by RT-PCR method using specific primers.<p>
<b>Results: </b>In this study, in consequence of analyzing 30 samples from 15 apiaries
by RT-PCR method, SBV was detected in 10 samples obtained from 5
apiaries (33.3%) and CBPV was found in 4 samples from 2 apiaries (13.3.%).
IAPV and KBV were not detected. As a result of evaluations, 4 samples from 2
apiaries were considered to have been infected with both SBV and CBPV, which
means a mixed infection occurred in these hives.<p>
<b>Conclusion:</b> It is clear that the so-called diseases were regarded important in
terms of beekeeping in not only Burdur region but also in the other regions of
Turkey and serious precautions should be taken to prevent infections. Struggling
with bee parasites that play the key role in transmission viral agents and
enhancing the immune resistance of bees and obeying the rules of hygiene and
sanitation should not be ignored in protecting bee health. Besides, the future
studies on honeybee viruses should be planned to include wider areas and
populations and to reveal viral phylogenies. |
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| ISSN: | 1309-6958 2146-1953 |