Biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regeneration

Background: The ultimate goal of tissue engineering is to reproduce functional alveolar∖Periodontal complex. This study aimed to asses micro- and nano-formulated crystalline calcium sulfate as possible treatment of intrabony defect utilizing an autologous Platelet rich fibrin scaffold. Subjects: and...

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Main Authors: Abdulkarem A. Mohammed, Amira M. Elsherbini, Fatma M. Ibrahim, Samah M. El-Meadawy, Jilan M. Youssef
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:Journal of Oral Biology and Craniofacial Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2212426820301561
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author Abdulkarem A. Mohammed
Amira M. Elsherbini
Fatma M. Ibrahim
Samah M. El-Meadawy
Jilan M. Youssef
author_facet Abdulkarem A. Mohammed
Amira M. Elsherbini
Fatma M. Ibrahim
Samah M. El-Meadawy
Jilan M. Youssef
author_sort Abdulkarem A. Mohammed
collection DOAJ
description Background: The ultimate goal of tissue engineering is to reproduce functional alveolar∖Periodontal complex. This study aimed to asses micro- and nano-formulated crystalline calcium sulfate as possible treatment of intrabony defect utilizing an autologous Platelet rich fibrin scaffold. Subjects: and Methods: An experimental prospective split mouth design using eight healthy mongrel dog’s was conducted. After two months of extracting the first premolar, bilateral intrabony defects were created mesial to the mandibular second premolar. One side was grafted by nanocrystalline calcium sulfate, while, the opposite side was grafted with microcrystalline calcium sulfate graft. Dogs were randomly euthanized after one month (group I) and 3 months (group II). The primary outcomes were the histological changes of new bone formation in hematoxylin and eosin and Masson trichrome stains, while histomorphometric and radiographic analysis were the secondary outcome followed by statistical analysis (P value was set at .05). Results: There was a significant increase in the percentage of bone formation in relation to defect height in nanocrystalline compared to microcrystalline form after one month and three months in both histological (p-values of <0.0001) and radiographic results (p-values of <0.0001). Conclusion: Nanocrystalline calcium sulfate presented a significant enhanced periodontal regeneration compared to the microcrystalline form.
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issn 2212-4268
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publishDate 2021-01-01
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series Journal of Oral Biology and Craniofacial Research
spelling doaj-art-ee915a01d54e4bfe817ddd9ab2925f8e2024-11-23T06:28:27ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682021-01-011114752Biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regenerationAbdulkarem A. Mohammed0Amira M. Elsherbini1Fatma M. Ibrahim2Samah M. El-Meadawy3Jilan M. Youssef4Oral Medicine and Periodontology, Faculty of Dentistry, Thamar University, Yemen; Oral Medicine and Periodontology, Faculty of Dentistry, Mansoura University, EgyptOral Biology, Faculty of Dentistry, Mansoura University, Egypt; Corresponding author. Lecturer of Oral Biology, Faculty of Dentistry, Mansoura University, 35511, 25 El Gomhouria St, Dakahlia Governorate, Egypt.Oral Biology, Faculty of Dentistry, Mansoura University, EgyptOral Medicine and Periodontology, Faculty of Dentistry, Mansoura University, EgyptOral Medicine and Periodontology, Faculty of Dentistry, Mansoura University, EgyptBackground: The ultimate goal of tissue engineering is to reproduce functional alveolar∖Periodontal complex. This study aimed to asses micro- and nano-formulated crystalline calcium sulfate as possible treatment of intrabony defect utilizing an autologous Platelet rich fibrin scaffold. Subjects: and Methods: An experimental prospective split mouth design using eight healthy mongrel dog’s was conducted. After two months of extracting the first premolar, bilateral intrabony defects were created mesial to the mandibular second premolar. One side was grafted by nanocrystalline calcium sulfate, while, the opposite side was grafted with microcrystalline calcium sulfate graft. Dogs were randomly euthanized after one month (group I) and 3 months (group II). The primary outcomes were the histological changes of new bone formation in hematoxylin and eosin and Masson trichrome stains, while histomorphometric and radiographic analysis were the secondary outcome followed by statistical analysis (P value was set at .05). Results: There was a significant increase in the percentage of bone formation in relation to defect height in nanocrystalline compared to microcrystalline form after one month and three months in both histological (p-values of <0.0001) and radiographic results (p-values of <0.0001). Conclusion: Nanocrystalline calcium sulfate presented a significant enhanced periodontal regeneration compared to the microcrystalline form.http://www.sciencedirect.com/science/article/pii/S2212426820301561Platelet rich fibrinMicrocrystalline calcium sulfateNanocrystalline calcium sulfatePeriodontal defectOne wall intrabony defect
spellingShingle Abdulkarem A. Mohammed
Amira M. Elsherbini
Fatma M. Ibrahim
Samah M. El-Meadawy
Jilan M. Youssef
Biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regeneration
Journal of Oral Biology and Craniofacial Research
Platelet rich fibrin
Microcrystalline calcium sulfate
Nanocrystalline calcium sulfate
Periodontal defect
One wall intrabony defect
title Biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regeneration
title_full Biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regeneration
title_fullStr Biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regeneration
title_full_unstemmed Biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regeneration
title_short Biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regeneration
title_sort biological effect of the nanocrystalline calcium sulfate bone graft in the periodontal regeneration
topic Platelet rich fibrin
Microcrystalline calcium sulfate
Nanocrystalline calcium sulfate
Periodontal defect
One wall intrabony defect
url http://www.sciencedirect.com/science/article/pii/S2212426820301561
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AT fatmamibrahim biologicaleffectofthenanocrystallinecalciumsulfatebonegraftintheperiodontalregeneration
AT samahmelmeadawy biologicaleffectofthenanocrystallinecalciumsulfatebonegraftintheperiodontalregeneration
AT jilanmyoussef biologicaleffectofthenanocrystallinecalciumsulfatebonegraftintheperiodontalregeneration