Construction of the <i>Pseudomonas putida</i> Strain with Low Motility and Reduced Biofilm Formation for Application in Fermentation
<i>Pseudomonas putida</i> PCL1760 is a well-studied bacterium that can be used for a wide range of biotechnological applications. In our study we investigated the effect of deletion of the genes involved in alginate accumulation (<i>alg</i>A), flagellar export (<i>flh&l...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/10/12/606 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846104751902556160 |
|---|---|
| author | Mikhail Frolov Galim Alimzhanovich Kungurov Emil Elmirovich Valiakhmetov Artur Sergeyevich Gogov Natalia Viktorovna Trachtmann Shamil Zavdatovich Validov |
| author_facet | Mikhail Frolov Galim Alimzhanovich Kungurov Emil Elmirovich Valiakhmetov Artur Sergeyevich Gogov Natalia Viktorovna Trachtmann Shamil Zavdatovich Validov |
| author_sort | Mikhail Frolov |
| collection | DOAJ |
| description | <i>Pseudomonas putida</i> PCL1760 is a well-studied bacterium that can be used for a wide range of biotechnological applications. In our study we investigated the effect of deletion of the genes involved in alginate accumulation (<i>alg</i>A), flagellar export (<i>flh</i>A), and pili formation <i>pil</i>Q on the behavior of the strain in bioreactors. We obtained the knockout mutant P. putida LN6160 with the deletion of these genes and showed that the absence of these genes reduces mobility and biofilm formation (40% lower after 72 h) in the mutant. At the same time, we noted the positive effect of these deletions on the growth of the mutant strain on rich medium (1.39 × 10<sup>10</sup> CFU/mL in the mutant and 6.4 × 10<sup>9</sup> CFU/mL in the wild type) and on mineral medium (6.11 × 10<sup>9</sup> CFU/mL in the mutant and 1.36 × 10<sup>9</sup> CFU/mL in the wild type) by growing them in small-volume bioreactors. A significant decrease in the biofilm and the foam formation was also observed for LN6160 in a small-volume bioreactor. Most probably, the rapid growth of the deletion strain occurs due to a decrease in the energy load on the bacterial apparatus. |
| format | Article |
| id | doaj-art-ee90e13f3a2b452cb75a5947d4d1be8d |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-ee90e13f3a2b452cb75a5947d4d1be8d2024-12-27T14:25:24ZengMDPI AGFermentation2311-56372024-11-01101260610.3390/fermentation10120606Construction of the <i>Pseudomonas putida</i> Strain with Low Motility and Reduced Biofilm Formation for Application in FermentationMikhail Frolov0Galim Alimzhanovich Kungurov1Emil Elmirovich Valiakhmetov2Artur Sergeyevich Gogov3Natalia Viktorovna Trachtmann4Shamil Zavdatovich Validov5Laboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, Orenburgskiy Tract 20A, 420059 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, Orenburgskiy Tract 20A, 420059 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, Orenburgskiy Tract 20A, 420059 Kazan, RussiaNational Research Center Kurchatov Institute, po. Akademika Kurchatova, v. 1, 123182 Moscow, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, Orenburgskiy Tract 20A, 420059 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, Orenburgskiy Tract 20A, 420059 Kazan, Russia<i>Pseudomonas putida</i> PCL1760 is a well-studied bacterium that can be used for a wide range of biotechnological applications. In our study we investigated the effect of deletion of the genes involved in alginate accumulation (<i>alg</i>A), flagellar export (<i>flh</i>A), and pili formation <i>pil</i>Q on the behavior of the strain in bioreactors. We obtained the knockout mutant P. putida LN6160 with the deletion of these genes and showed that the absence of these genes reduces mobility and biofilm formation (40% lower after 72 h) in the mutant. At the same time, we noted the positive effect of these deletions on the growth of the mutant strain on rich medium (1.39 × 10<sup>10</sup> CFU/mL in the mutant and 6.4 × 10<sup>9</sup> CFU/mL in the wild type) and on mineral medium (6.11 × 10<sup>9</sup> CFU/mL in the mutant and 1.36 × 10<sup>9</sup> CFU/mL in the wild type) by growing them in small-volume bioreactors. A significant decrease in the biofilm and the foam formation was also observed for LN6160 in a small-volume bioreactor. Most probably, the rapid growth of the deletion strain occurs due to a decrease in the energy load on the bacterial apparatus.https://www.mdpi.com/2311-5637/10/12/606<i>Pseudomonas putida</i>bioreactorfermentationbiofilm formationmotilitypilQ |
| spellingShingle | Mikhail Frolov Galim Alimzhanovich Kungurov Emil Elmirovich Valiakhmetov Artur Sergeyevich Gogov Natalia Viktorovna Trachtmann Shamil Zavdatovich Validov Construction of the <i>Pseudomonas putida</i> Strain with Low Motility and Reduced Biofilm Formation for Application in Fermentation Fermentation <i>Pseudomonas putida</i> bioreactor fermentation biofilm formation motility pilQ |
| title | Construction of the <i>Pseudomonas putida</i> Strain with Low Motility and Reduced Biofilm Formation for Application in Fermentation |
| title_full | Construction of the <i>Pseudomonas putida</i> Strain with Low Motility and Reduced Biofilm Formation for Application in Fermentation |
| title_fullStr | Construction of the <i>Pseudomonas putida</i> Strain with Low Motility and Reduced Biofilm Formation for Application in Fermentation |
| title_full_unstemmed | Construction of the <i>Pseudomonas putida</i> Strain with Low Motility and Reduced Biofilm Formation for Application in Fermentation |
| title_short | Construction of the <i>Pseudomonas putida</i> Strain with Low Motility and Reduced Biofilm Formation for Application in Fermentation |
| title_sort | construction of the i pseudomonas putida i strain with low motility and reduced biofilm formation for application in fermentation |
| topic | <i>Pseudomonas putida</i> bioreactor fermentation biofilm formation motility pilQ |
| url | https://www.mdpi.com/2311-5637/10/12/606 |
| work_keys_str_mv | AT mikhailfrolov constructionoftheipseudomonasputidaistrainwithlowmotilityandreducedbiofilmformationforapplicationinfermentation AT galimalimzhanovichkungurov constructionoftheipseudomonasputidaistrainwithlowmotilityandreducedbiofilmformationforapplicationinfermentation AT emilelmirovichvaliakhmetov constructionoftheipseudomonasputidaistrainwithlowmotilityandreducedbiofilmformationforapplicationinfermentation AT artursergeyevichgogov constructionoftheipseudomonasputidaistrainwithlowmotilityandreducedbiofilmformationforapplicationinfermentation AT nataliaviktorovnatrachtmann constructionoftheipseudomonasputidaistrainwithlowmotilityandreducedbiofilmformationforapplicationinfermentation AT shamilzavdatovichvalidov constructionoftheipseudomonasputidaistrainwithlowmotilityandreducedbiofilmformationforapplicationinfermentation |