Analysis of Microbial Community Heterogeneity and Carbon Fixation Capabilities in Oil-Contaminated Soils in Chinese Onshore Oilfields
This study selected 27 soil samples from four representative horizontally distributed onshore oilfields in China to explore the diversity of soil microbial communities and their carbon fixation capacity, with a focus on the potential interaction between pollution and carbon fixation under oil pollut...
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2024-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/11/2379 |
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| author | Jiayu Song Yakui Chen Yilei Han Yunzhao Li Zheng Liu Xingchun Li Diannan Lu Chunmao Chen |
| author_facet | Jiayu Song Yakui Chen Yilei Han Yunzhao Li Zheng Liu Xingchun Li Diannan Lu Chunmao Chen |
| author_sort | Jiayu Song |
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| description | This study selected 27 soil samples from four representative horizontally distributed onshore oilfields in China to explore the diversity of soil microbial communities and their carbon fixation capacity, with a focus on the potential interaction between pollution and carbon fixation under oil pollution stress. The analysis of the soil physicochemical properties and microbial community structures from these oilfield samples confirmed a clear biogeographic isolation effect, indicating spatial heterogeneity in the microbial communities. Additionally, the key factors influencing microbial community composition differed across regions. The dominant bacterial phyla of soil microorganisms under soil pollution stress were Proteobacteria, Actinobacteriota, Chloroflexi, Acidobacteriota, Firmicutes, Bacteroidota, and Gemmatimonadota. A correlation network analysis identified <i>Immundisolibacter</i>, <i>Acinetobacter</i>, <i>Blastococcus</i>, <i>Truepera</i>, and <i>Kocuria</i> as key players in the microbial network, with most showing positive correlations. The results of the KEGG database functional annotation showed that degradation and carbon fixation metabolic pathways coexist in soil samples and maintain a balanced relative abundance. These metabolic pathways highlight the functional diversity of microorganisms. Among them, prokaryotic and eukaryotic carbon fixation pathways, along with benzoate degradation pathways, are predominant. These findings establish a theoretical basis for further exploration of the synergistic mechanisms underlying pollution reduction and carbon sequestration by microorganisms in petroleum-contaminated soils. |
| format | Article |
| id | doaj-art-ee87dd4b64e148b295acb58070a65b04 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-ee87dd4b64e148b295acb58070a65b042024-11-26T18:15:10ZengMDPI AGMicroorganisms2076-26072024-11-011211237910.3390/microorganisms12112379Analysis of Microbial Community Heterogeneity and Carbon Fixation Capabilities in Oil-Contaminated Soils in Chinese Onshore OilfieldsJiayu Song0Yakui Chen1Yilei Han2Yunzhao Li3Zheng Liu4Xingchun Li5Diannan Lu6Chunmao Chen7State Key Laboratory of Petroleum Pollution Control, Beijing 102206, ChinaDepartment of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaCollege of Chemical Engineering and Environment, China University of Petroleum-Beijing, Beijing 102206, ChinaDepartment of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaState Key Laboratory of Petroleum Pollution Control, Beijing 102206, ChinaDepartment of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaCollege of Chemical Engineering and Environment, China University of Petroleum-Beijing, Beijing 102206, ChinaThis study selected 27 soil samples from four representative horizontally distributed onshore oilfields in China to explore the diversity of soil microbial communities and their carbon fixation capacity, with a focus on the potential interaction between pollution and carbon fixation under oil pollution stress. The analysis of the soil physicochemical properties and microbial community structures from these oilfield samples confirmed a clear biogeographic isolation effect, indicating spatial heterogeneity in the microbial communities. Additionally, the key factors influencing microbial community composition differed across regions. The dominant bacterial phyla of soil microorganisms under soil pollution stress were Proteobacteria, Actinobacteriota, Chloroflexi, Acidobacteriota, Firmicutes, Bacteroidota, and Gemmatimonadota. A correlation network analysis identified <i>Immundisolibacter</i>, <i>Acinetobacter</i>, <i>Blastococcus</i>, <i>Truepera</i>, and <i>Kocuria</i> as key players in the microbial network, with most showing positive correlations. The results of the KEGG database functional annotation showed that degradation and carbon fixation metabolic pathways coexist in soil samples and maintain a balanced relative abundance. These metabolic pathways highlight the functional diversity of microorganisms. Among them, prokaryotic and eukaryotic carbon fixation pathways, along with benzoate degradation pathways, are predominant. These findings establish a theoretical basis for further exploration of the synergistic mechanisms underlying pollution reduction and carbon sequestration by microorganisms in petroleum-contaminated soils.https://www.mdpi.com/2076-2607/12/11/2379oil-contaminated soilsmicrobial diversitycarbon fixationcorrelation networkpetroleum hydrocarbon degradationmetabolic pathways |
| spellingShingle | Jiayu Song Yakui Chen Yilei Han Yunzhao Li Zheng Liu Xingchun Li Diannan Lu Chunmao Chen Analysis of Microbial Community Heterogeneity and Carbon Fixation Capabilities in Oil-Contaminated Soils in Chinese Onshore Oilfields Microorganisms oil-contaminated soils microbial diversity carbon fixation correlation network petroleum hydrocarbon degradation metabolic pathways |
| title | Analysis of Microbial Community Heterogeneity and Carbon Fixation Capabilities in Oil-Contaminated Soils in Chinese Onshore Oilfields |
| title_full | Analysis of Microbial Community Heterogeneity and Carbon Fixation Capabilities in Oil-Contaminated Soils in Chinese Onshore Oilfields |
| title_fullStr | Analysis of Microbial Community Heterogeneity and Carbon Fixation Capabilities in Oil-Contaminated Soils in Chinese Onshore Oilfields |
| title_full_unstemmed | Analysis of Microbial Community Heterogeneity and Carbon Fixation Capabilities in Oil-Contaminated Soils in Chinese Onshore Oilfields |
| title_short | Analysis of Microbial Community Heterogeneity and Carbon Fixation Capabilities in Oil-Contaminated Soils in Chinese Onshore Oilfields |
| title_sort | analysis of microbial community heterogeneity and carbon fixation capabilities in oil contaminated soils in chinese onshore oilfields |
| topic | oil-contaminated soils microbial diversity carbon fixation correlation network petroleum hydrocarbon degradation metabolic pathways |
| url | https://www.mdpi.com/2076-2607/12/11/2379 |
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