Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with CaMYC2 positively regulate capsaicinoid biosynthesis in pepper (Capsicum annuum)
The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis. Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits. However, the transcriptional regulation of capsaicinoid biosynthesis remains largely u...
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KeAi Communications Co., Ltd.
2025-01-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014123001498 |
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| author | Jiali Song Renjian Liu Guoju Chen Jianjun Lei Zuoyang Ning Xiangru Tang Xiaowan Xu Muxi Chen Bihao Cao Changming Chen Zhangsheng Zhu |
| author_facet | Jiali Song Renjian Liu Guoju Chen Jianjun Lei Zuoyang Ning Xiangru Tang Xiaowan Xu Muxi Chen Bihao Cao Changming Chen Zhangsheng Zhu |
| author_sort | Jiali Song |
| collection | DOAJ |
| description | The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis. Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits. However, the transcriptional regulation of capsaicinoid biosynthesis remains largely unknown. In this study, two AP2/ERF transcription factors (TFs), CaERF102 and CaERF111, were characterized for their role in the capsaicinoid biosynthesis process. Expression analysis of two ERFs and capsaicinoid biosynthetic genes (CBGs) suggested that they were associated with capsaicinoid biosynthesis. Both ERFs encode nuclear-localized proteins and function as transcriptional activators through their C-terminal activation motifs. The two ERF TFs participated in capsaicinoid biosynthesis by directly activating the promoters of key CBGs, and this activation was significantly enhanced when CaMYC2 was co-expressed. Moreover, CaERF102 and CaERF111 were found to interact with CaMYC2. This study helps elucidate the AP2/ERF TF regulatory network that governs capsaicinoid biosynthesis in Capsicum species. |
| format | Article |
| id | doaj-art-ee7e33fccd3b4b72b73be2b1ab203813 |
| institution | Kabale University |
| issn | 2468-0141 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj-art-ee7e33fccd3b4b72b73be2b1ab2038132025-01-01T05:10:55ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412025-01-01111275289Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with CaMYC2 positively regulate capsaicinoid biosynthesis in pepper (Capsicum annuum)Jiali Song0Renjian Liu1Guoju Chen2Jianjun Lei3Zuoyang Ning4Xiangru Tang5Xiaowan Xu6Muxi Chen7Bihao Cao8Changming Chen9Zhangsheng Zhu10Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Vegetable Engineering and Technology Research Center, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Vegetable Engineering and Technology Research Center, South China Agricultural University, Guangzhou, Guangdong 510642, China; Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan, Guangdong 512005, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou, Guangdong, 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Vegetable Engineering and Technology Research Center, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Helinong Seeds, CO., LTD., Shantou, Guangdong 515800, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Vegetable Engineering and Technology Research Center, South China Agricultural University, Guangzhou, Guangdong 510642, China; Corresponding authors.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Vegetable Engineering and Technology Research Center, South China Agricultural University, Guangzhou, Guangdong 510642, China; Corresponding authors.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Vegetable Engineering and Technology Research Center, South China Agricultural University, Guangzhou, Guangdong 510642, China; Corresponding authors.The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis. Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits. However, the transcriptional regulation of capsaicinoid biosynthesis remains largely unknown. In this study, two AP2/ERF transcription factors (TFs), CaERF102 and CaERF111, were characterized for their role in the capsaicinoid biosynthesis process. Expression analysis of two ERFs and capsaicinoid biosynthetic genes (CBGs) suggested that they were associated with capsaicinoid biosynthesis. Both ERFs encode nuclear-localized proteins and function as transcriptional activators through their C-terminal activation motifs. The two ERF TFs participated in capsaicinoid biosynthesis by directly activating the promoters of key CBGs, and this activation was significantly enhanced when CaMYC2 was co-expressed. Moreover, CaERF102 and CaERF111 were found to interact with CaMYC2. This study helps elucidate the AP2/ERF TF regulatory network that governs capsaicinoid biosynthesis in Capsicum species.http://www.sciencedirect.com/science/article/pii/S2468014123001498CapsicumSpecialized metabolitesPungencyTranscription factorAP2/ERFMYC |
| spellingShingle | Jiali Song Renjian Liu Guoju Chen Jianjun Lei Zuoyang Ning Xiangru Tang Xiaowan Xu Muxi Chen Bihao Cao Changming Chen Zhangsheng Zhu Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with CaMYC2 positively regulate capsaicinoid biosynthesis in pepper (Capsicum annuum) Horticultural Plant Journal Capsicum Specialized metabolites Pungency Transcription factor AP2/ERF MYC |
| title | Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with CaMYC2 positively regulate capsaicinoid biosynthesis in pepper (Capsicum annuum) |
| title_full | Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with CaMYC2 positively regulate capsaicinoid biosynthesis in pepper (Capsicum annuum) |
| title_fullStr | Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with CaMYC2 positively regulate capsaicinoid biosynthesis in pepper (Capsicum annuum) |
| title_full_unstemmed | Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with CaMYC2 positively regulate capsaicinoid biosynthesis in pepper (Capsicum annuum) |
| title_short | Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with CaMYC2 positively regulate capsaicinoid biosynthesis in pepper (Capsicum annuum) |
| title_sort | two apetala2 ethylene response factors coordinately with camyc2 positively regulate capsaicinoid biosynthesis in pepper capsicum annuum |
| topic | Capsicum Specialized metabolites Pungency Transcription factor AP2/ERF MYC |
| url | http://www.sciencedirect.com/science/article/pii/S2468014123001498 |
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