Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides
Summary: Hydrogels with spatial-temporal control over chemical and physical properties allow for the creation of cellular niches with controllable properties that better mimic tissue environments. Here, we present a protocol for synthesizing hydrogels incorporating photocaged oligonucleotides that c...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724006671 |
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| author | Danielle Herubin Amanda Yang Saanvi Gaddam Katelyn Mathis Brian Meckes |
| author_facet | Danielle Herubin Amanda Yang Saanvi Gaddam Katelyn Mathis Brian Meckes |
| author_sort | Danielle Herubin |
| collection | DOAJ |
| description | Summary: Hydrogels with spatial-temporal control over chemical and physical properties allow for the creation of cellular niches with controllable properties that better mimic tissue environments. Here, we present a protocol for synthesizing hydrogels incorporating photocaged oligonucleotides that can be activated with non-ultraviolet (UV) wavelengths. We detail the synthesis of bulk hydrogels and spatially defined hydrogels with different chemical functionalities that all share common photocaged DNA. Furthermore, we describe conditions for activating photocaged DNA to capture complementary oligonucleotides.For complete details on the use and execution of this protocol, please refer to Mathis et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-ee4d1cda693a4762a3f9f219468c5c48 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-ee4d1cda693a4762a3f9f219468c5c482024-12-18T08:52:08ZengElsevierSTAR Protocols2666-16672025-03-0161103502Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotidesDanielle Herubin0Amanda Yang1Saanvi Gaddam2Katelyn Mathis3Brian Meckes4Department of Biomedical Engineering, University of North Texas, Denton, TX 76207, USA; Biodiscovery Institute, University of North Texas, Denton, TX 76205, USADepartment of Biomedical Engineering, University of North Texas, Denton, TX 76207, USA; Texas Academy of Mathematics and Science, University of North Texas, Denton, TX 76205, USADepartment of Biomedical Engineering, University of North Texas, Denton, TX 76207, USA; Texas Academy of Mathematics and Science, University of North Texas, Denton, TX 76205, USADepartment of Biomedical Engineering, University of North Texas, Denton, TX 76207, USA; Biodiscovery Institute, University of North Texas, Denton, TX 76205, USA; Corresponding authorDepartment of Biomedical Engineering, University of North Texas, Denton, TX 76207, USA; Biodiscovery Institute, University of North Texas, Denton, TX 76205, USA; Corresponding authorSummary: Hydrogels with spatial-temporal control over chemical and physical properties allow for the creation of cellular niches with controllable properties that better mimic tissue environments. Here, we present a protocol for synthesizing hydrogels incorporating photocaged oligonucleotides that can be activated with non-ultraviolet (UV) wavelengths. We detail the synthesis of bulk hydrogels and spatially defined hydrogels with different chemical functionalities that all share common photocaged DNA. Furthermore, we describe conditions for activating photocaged DNA to capture complementary oligonucleotides.For complete details on the use and execution of this protocol, please refer to Mathis et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724006671Tissue EngineeringBiotechnology and bioengineeringChemistryMaterial sciences |
| spellingShingle | Danielle Herubin Amanda Yang Saanvi Gaddam Katelyn Mathis Brian Meckes Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides STAR Protocols Tissue Engineering Biotechnology and bioengineering Chemistry Material sciences |
| title | Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides |
| title_full | Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides |
| title_fullStr | Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides |
| title_full_unstemmed | Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides |
| title_short | Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides |
| title_sort | protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides |
| topic | Tissue Engineering Biotechnology and bioengineering Chemistry Material sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724006671 |
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