The Role of circTmeff-1 in Morphine Addiction Memory of Mice
In addition to the essential pharmacological effects of opioids, situational cues associated with drug addiction memory are key triggers for drug seeking. CircRNAs, an emerging hotspot regulator in crown genetics, play an important role in central nervous system-related diseases. However, the intern...
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2023-08-01
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| author | Hailei Yu Boyang Wen Yun Lu Bing Xie Feng Yu Minglong Zhang Chunling Ma Bin Cong Di Wen Haitao Bi |
| author_facet | Hailei Yu Boyang Wen Yun Lu Bing Xie Feng Yu Minglong Zhang Chunling Ma Bin Cong Di Wen Haitao Bi |
| author_sort | Hailei Yu |
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| description | In addition to the essential pharmacological effects of opioids, situational cues associated with drug addiction memory are key triggers for drug seeking. CircRNAs, an emerging hotspot regulator in crown genetics, play an important role in central nervous system-related diseases. However, the internal mediating mechanism of circRNAs in the field of drug reward and addiction memory remains unknown. Here, we trained mice on a conditional place preference (CPP) model and collected nucleus accumbens (NAc) tissues from day 1 (T0) and day 8 (T1) for high-throughput RNA sequencing. QRT-PCR analysis revealed that circTmeff-1 was highly expressed in the NAc core but not in the NAc shell, suggesting that it plays a role in addiction memory formation. Meanwhile, the down-regulation of circTmeff-1 by adeno-associated viruses in the NAc core or shell could inhibit the morphine CPP scores. Subsequently, the GO and KEGG analyses indicated that circTmeff-1 might regulate the addiction memory via the MAPK and AMPK pathways. These findings suggest that circTmeff-1 in NAc plays a crucial role in morphine-dependent memory formation. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2023-08-01 |
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| series | Cells |
| spelling | doaj-art-ee30a2799ae24b109e8b91eec0b92b5d2025-01-17T13:52:46ZengMDPI AGCells2073-44092023-08-011215198510.3390/cells12151985The Role of circTmeff-1 in Morphine Addiction Memory of MiceHailei Yu0Boyang Wen1Yun Lu2Bing Xie3Feng Yu4Minglong Zhang5Chunling Ma6Bin Cong7Di Wen8Haitao Bi9Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaHebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaHebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaHebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaHebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaDepartment of Biogenetics, Qiqihar Medical University, Qiqihar 161000, ChinaHebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaHebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaHebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaHebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, College of Forensic Medicine, Hebei Medical University, Chinese Academy of Medical Sciences, Shijiazhuang 050000, ChinaIn addition to the essential pharmacological effects of opioids, situational cues associated with drug addiction memory are key triggers for drug seeking. CircRNAs, an emerging hotspot regulator in crown genetics, play an important role in central nervous system-related diseases. However, the internal mediating mechanism of circRNAs in the field of drug reward and addiction memory remains unknown. Here, we trained mice on a conditional place preference (CPP) model and collected nucleus accumbens (NAc) tissues from day 1 (T0) and day 8 (T1) for high-throughput RNA sequencing. QRT-PCR analysis revealed that circTmeff-1 was highly expressed in the NAc core but not in the NAc shell, suggesting that it plays a role in addiction memory formation. Meanwhile, the down-regulation of circTmeff-1 by adeno-associated viruses in the NAc core or shell could inhibit the morphine CPP scores. Subsequently, the GO and KEGG analyses indicated that circTmeff-1 might regulate the addiction memory via the MAPK and AMPK pathways. These findings suggest that circTmeff-1 in NAc plays a crucial role in morphine-dependent memory formation.https://www.mdpi.com/2073-4409/12/15/1985circRNAsmorphineconditional place preferenceaddiction memorynucleus accumbens |
| spellingShingle | Hailei Yu Boyang Wen Yun Lu Bing Xie Feng Yu Minglong Zhang Chunling Ma Bin Cong Di Wen Haitao Bi The Role of circTmeff-1 in Morphine Addiction Memory of Mice Cells circRNAs morphine conditional place preference addiction memory nucleus accumbens |
| title | The Role of circTmeff-1 in Morphine Addiction Memory of Mice |
| title_full | The Role of circTmeff-1 in Morphine Addiction Memory of Mice |
| title_fullStr | The Role of circTmeff-1 in Morphine Addiction Memory of Mice |
| title_full_unstemmed | The Role of circTmeff-1 in Morphine Addiction Memory of Mice |
| title_short | The Role of circTmeff-1 in Morphine Addiction Memory of Mice |
| title_sort | role of circtmeff 1 in morphine addiction memory of mice |
| topic | circRNAs morphine conditional place preference addiction memory nucleus accumbens |
| url | https://www.mdpi.com/2073-4409/12/15/1985 |
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