Fungal Endophytes Diversity and Influencing Factors in Liquidambar orientalis Mill. in Türkiye
Fungal endophytes were isolated from the leaves and petioles of Liquidambar orientalis Mill., an endangered species in Türkiye. Plant material was collected from 10 sites in September 2023, yielding 499 fungal isolates, classified into 38 morphological groups. DNA extraction and polymerase chain rea...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2024-12-01
|
| Series: | BioResources |
| Subjects: | |
| Online Access: | https://ojs.bioresources.com/index.php/BRJ/article/view/24107 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846093265867112448 |
|---|---|
| author | Refika Ceyda Beram Sultan Akyol |
| author_facet | Refika Ceyda Beram Sultan Akyol |
| author_sort | Refika Ceyda Beram |
| collection | DOAJ |
| description | Fungal endophytes were isolated from the leaves and petioles of Liquidambar orientalis Mill., an endangered species in Türkiye. Plant material was collected from 10 sites in September 2023, yielding 499 fungal isolates, classified into 38 morphological groups. DNA extraction and polymerase chain reaction (PCR) amplification of ITS and Beta-tubulin regions were conducted on representative isolates. All fungi belonged to the Ascomycota phylum, comprising 11 genera and 26 species across 15 families, with one group unidentified. The most prevalent families were Diaporthaceae (34.9%), Pleosporaceae (23.4%), and Botryosphaeriaceae (22.2%), with Diaporthe eres (15.0%) and Phomopsis sp. (12.4%) being dominant species. Fungal diversity was assessed using Shannon, Simpson, and Chao1 indices, revealing tissue type as the strongest factor influencing species diversity, followed by media and spatial factors. The presence of pathogenic families, such as Botryosphaeriaceae, highlights potential threats to the species. This is the first study to report fungal endophytes in L. orientalis, as well as the first records in Türkiye for several species, including Alternaria destruens, Alternaria alstroemeriae, Stemphylium majusculum, Diaporthe cynaroidis, Pseudopithomyces rosae, Nothophoma variabilis, Cladosporium endophyticum, Cladosporium colombiae, Muyocopron sp., Sphaerulina rhododendricola, Constantinomyces macerans, and Aequabiliella effusa. |
| format | Article |
| id | doaj-art-ee24a6cdec3148dbb5f59b2fcce3e53b |
| institution | Kabale University |
| issn | 1930-2126 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj-art-ee24a6cdec3148dbb5f59b2fcce3e53b2025-01-02T18:23:13ZengNorth Carolina State UniversityBioResources1930-21262024-12-01201125112722344Fungal Endophytes Diversity and Influencing Factors in Liquidambar orientalis Mill. in TürkiyeRefika Ceyda Beram0https://orcid.org/0000-0002-1203-2910Sultan Akyol1https://orcid.org/0000-0003-4440-2960Pamukkale University, Faculty of Science, Department of Biology, 20160, Denizli, TürkiyePamukkale University, Faculty of Science, Department of Biology, 20160, Denizli, TürkiyeFungal endophytes were isolated from the leaves and petioles of Liquidambar orientalis Mill., an endangered species in Türkiye. Plant material was collected from 10 sites in September 2023, yielding 499 fungal isolates, classified into 38 morphological groups. DNA extraction and polymerase chain reaction (PCR) amplification of ITS and Beta-tubulin regions were conducted on representative isolates. All fungi belonged to the Ascomycota phylum, comprising 11 genera and 26 species across 15 families, with one group unidentified. The most prevalent families were Diaporthaceae (34.9%), Pleosporaceae (23.4%), and Botryosphaeriaceae (22.2%), with Diaporthe eres (15.0%) and Phomopsis sp. (12.4%) being dominant species. Fungal diversity was assessed using Shannon, Simpson, and Chao1 indices, revealing tissue type as the strongest factor influencing species diversity, followed by media and spatial factors. The presence of pathogenic families, such as Botryosphaeriaceae, highlights potential threats to the species. This is the first study to report fungal endophytes in L. orientalis, as well as the first records in Türkiye for several species, including Alternaria destruens, Alternaria alstroemeriae, Stemphylium majusculum, Diaporthe cynaroidis, Pseudopithomyces rosae, Nothophoma variabilis, Cladosporium endophyticum, Cladosporium colombiae, Muyocopron sp., Sphaerulina rhododendricola, Constantinomyces macerans, and Aequabiliella effusa.https://ojs.bioresources.com/index.php/BRJ/article/view/24107anatolian sweetgumendophytic fungifungal pathogenmycobiotatürkiye |
| spellingShingle | Refika Ceyda Beram Sultan Akyol Fungal Endophytes Diversity and Influencing Factors in Liquidambar orientalis Mill. in Türkiye BioResources anatolian sweetgum endophytic fungi fungal pathogen mycobiota türkiye |
| title | Fungal Endophytes Diversity and Influencing Factors in Liquidambar orientalis Mill. in Türkiye |
| title_full | Fungal Endophytes Diversity and Influencing Factors in Liquidambar orientalis Mill. in Türkiye |
| title_fullStr | Fungal Endophytes Diversity and Influencing Factors in Liquidambar orientalis Mill. in Türkiye |
| title_full_unstemmed | Fungal Endophytes Diversity and Influencing Factors in Liquidambar orientalis Mill. in Türkiye |
| title_short | Fungal Endophytes Diversity and Influencing Factors in Liquidambar orientalis Mill. in Türkiye |
| title_sort | fungal endophytes diversity and influencing factors in liquidambar orientalis mill in turkiye |
| topic | anatolian sweetgum endophytic fungi fungal pathogen mycobiota türkiye |
| url | https://ojs.bioresources.com/index.php/BRJ/article/view/24107 |
| work_keys_str_mv | AT refikaceydaberam fungalendophytesdiversityandinfluencingfactorsinliquidambarorientalismillinturkiye AT sultanakyol fungalendophytesdiversityandinfluencingfactorsinliquidambarorientalismillinturkiye |