The effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded land

Abstract Rehabilitating poor soil can be achieved in part by planting trees. In the field, bio-augmentation and organic amendments (OAs) promote plant establishment and growth. The experimental site is a fragile land due to its intrinsic characteristics and overexploitation which makes them abandone...

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Main Authors: Alemayehu Getahun, Diriba Muleta, Solomon Kiros, Fassil Assefa
Format: Article
Language:English
Published: Springer 2025-07-01
Series:Discover Applied Sciences
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Online Access:https://doi.org/10.1007/s42452-025-07539-4
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author Alemayehu Getahun
Diriba Muleta
Solomon Kiros
Fassil Assefa
author_facet Alemayehu Getahun
Diriba Muleta
Solomon Kiros
Fassil Assefa
author_sort Alemayehu Getahun
collection DOAJ
description Abstract Rehabilitating poor soil can be achieved in part by planting trees. In the field, bio-augmentation and organic amendments (OAs) promote plant establishment and growth. The experimental site is a fragile land due to its intrinsic characteristics and overexploitation which makes them abandoned (bare land without three). Biochar (coffee husk), compost (recycled floriculture) and manure (farmyard manure) are used as OAs. Moreover, mixed bacterial strains i.e., Pseudomonas fluorescent FB-49, Pseudomonas putida BS-19, Paenibacillus polymyxa BS-50, and Acinetobacter calcoaceticus BS-27 played a potential role in degraded land restoration. Hence, this study was initiated to assess the role of organic amendments and bacterial inoculations (BI) on tree establishment on degraded soil under field conditions. Six plots 41 m x 4 m were established to amend soil with organic matter and bacterial inoculation (BI) of the transplanted plants quarterly. Plots receiving OAs showed the highest counts of bacteria (21.66), actinomycetes (2.29), and fungi (0.82) x 105 colony-forming unit g−1 soil. Also, there were significant (p ≤ 0.05) increases in stem height (1, 0.42, and 0.39 m/plant), girths (12.0, 2.28, and 11.33 cm/plant), and branch numbers (5, 4, and 4.3/plant) on transplanted apple, acacia, and prunus respectively, compared to the control. The highest survival rate was observed on an apple tree (Malus domestica) (80%) and acacia (Acacia abyssinica) (66%). The result concluded that using OAs and BIs enhanced the growth and establishment of trees for the successful rehabilitation of degraded land.
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spelling doaj-art-ede0bdafb80c484d9036dc30d25c19a72025-08-20T03:46:24ZengSpringerDiscover Applied Sciences3004-92612025-07-017811610.1007/s42452-025-07539-4The effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded landAlemayehu Getahun0Diriba Muleta1Solomon Kiros2Fassil Assefa3College of Natural and Computational Sciences, Wachemo UniversityCollege of Natural and Computational Sciences, Addis Ababa UniversityAddis Ababa Institute of Technology, Addis Ababa UniversityCollege of Natural and Computational Sciences, Addis Ababa UniversityAbstract Rehabilitating poor soil can be achieved in part by planting trees. In the field, bio-augmentation and organic amendments (OAs) promote plant establishment and growth. The experimental site is a fragile land due to its intrinsic characteristics and overexploitation which makes them abandoned (bare land without three). Biochar (coffee husk), compost (recycled floriculture) and manure (farmyard manure) are used as OAs. Moreover, mixed bacterial strains i.e., Pseudomonas fluorescent FB-49, Pseudomonas putida BS-19, Paenibacillus polymyxa BS-50, and Acinetobacter calcoaceticus BS-27 played a potential role in degraded land restoration. Hence, this study was initiated to assess the role of organic amendments and bacterial inoculations (BI) on tree establishment on degraded soil under field conditions. Six plots 41 m x 4 m were established to amend soil with organic matter and bacterial inoculation (BI) of the transplanted plants quarterly. Plots receiving OAs showed the highest counts of bacteria (21.66), actinomycetes (2.29), and fungi (0.82) x 105 colony-forming unit g−1 soil. Also, there were significant (p ≤ 0.05) increases in stem height (1, 0.42, and 0.39 m/plant), girths (12.0, 2.28, and 11.33 cm/plant), and branch numbers (5, 4, and 4.3/plant) on transplanted apple, acacia, and prunus respectively, compared to the control. The highest survival rate was observed on an apple tree (Malus domestica) (80%) and acacia (Acacia abyssinica) (66%). The result concluded that using OAs and BIs enhanced the growth and establishment of trees for the successful rehabilitation of degraded land.https://doi.org/10.1007/s42452-025-07539-4AcaciaBio-augmentationBacterial countsRehabilitationSurvival rateTree
spellingShingle Alemayehu Getahun
Diriba Muleta
Solomon Kiros
Fassil Assefa
The effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded land
Discover Applied Sciences
Acacia
Bio-augmentation
Bacterial counts
Rehabilitation
Survival rate
Tree
title The effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded land
title_full The effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded land
title_fullStr The effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded land
title_full_unstemmed The effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded land
title_short The effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded land
title_sort effect of bacterial inoculation and organic amendments for the establishment of some multipurpose trees on degraded land
topic Acacia
Bio-augmentation
Bacterial counts
Rehabilitation
Survival rate
Tree
url https://doi.org/10.1007/s42452-025-07539-4
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