Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment
Objectives: to compare saliva proteome of patients before treatment of head and neck cancer and six months post-treatment with controls. Design: Five dentate patients and five age and gender-matched controls were included. The stimulated salivary secretion rate was determined, and saliva was stored...
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Elsevier
2024-10-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024150645 |
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| author | Ulrica Almhöjd Amela Fisic Hülya Cevik-Aras Lisa Tuomi Caterina Finizia Annica Almståhl |
| author_facet | Ulrica Almhöjd Amela Fisic Hülya Cevik-Aras Lisa Tuomi Caterina Finizia Annica Almståhl |
| author_sort | Ulrica Almhöjd |
| collection | DOAJ |
| description | Objectives: to compare saliva proteome of patients before treatment of head and neck cancer and six months post-treatment with controls. Design: Five dentate patients and five age and gender-matched controls were included. The stimulated salivary secretion rate was determined, and saliva was stored at −80 °C. After thawing, 30 mg of each sample and a reference (aliqouts of all samples) was trypsin digested. The digested peptides were analyzed by mass spectrometry. The relative abundances were transformed to log2 and significant differences determined. Relative abundances of mucins were compared with patient's problems with dry mouth, sticky saliva and swallowing. Data are available via ProteomeXchange with identifier PXD047500. Results: 966 proteins with ≥2 unique peptides were found. Compared with controls, 30 proteins were found in significantly lower relative abundances and 65 in higher at pre-treatment and 38 proteins in significantly lower relative abundances and 34 proteins in higher post-treatment. Regarding proteins from the salivary glands, a significantly lower relative abundance of Cystatins was detected pre-treatment and significantly lower relative abundances of Cystatin, Cysteine-rich secretory protein 3, Lactoperoxidase, Prolactin-inducible protein and Proline-rich protein 4 post-treatment. No clear relation between relative abundance of mucins and dry mouth, sticky saliva and problems with swallowing was detected. Conclusion: Decreases in several salivary gland proteins post cancer treatment might lead to a reduced defense against oral disorders. Knowledge about changes in saliva proteins in connection with oral cancer treatment is important for planning dental care for these patients. |
| format | Article |
| id | doaj-art-eda41ecb461a4ad7b5481e2a6ec4a8d3 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-eda41ecb461a4ad7b5481e2a6ec4a8d32024-11-12T05:19:36ZengElsevierHeliyon2405-84402024-10-011020e39033Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatmentUlrica Almhöjd0Amela Fisic1Hülya Cevik-Aras2Lisa Tuomi3Caterina Finizia4Annica Almståhl5Dept of Cariology, Institute of Odontology, Sahlgrenska academy, University of Gothenburg, SwedenSection 4- Oral health, Faculty of Odontology, Malmö University, SwedenDept of Oral Pathology and Medicine, Institute of Odontology, Sahlgrenska academy, University of Gothenburg, Sweden; Specialist Clinic for Orofacial Medicine, Norra Älvsborg County Hospital, Trollhättan, Public Dental Service, Region Västra Götaland, SwedenDept of Otorhinolaryngology, Head and Neck Surgery, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden; Institute of Neuroscience and Physiology, Speech and Language Pathology Unit, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDept of Otorhinolaryngology, Head and Neck Surgery, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden; Institute of Neuroscience and Physiology, Speech and Language Pathology Unit, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenSection 4- Oral health, Faculty of Odontology, Malmö University, Sweden; Dept of Oral Microbiology and Immunology, Institute of Odontology, Sahlgrenska academy, University of Gothenburg, Sweden; Corresponding author. Dept of Oral Microbiology and Immunology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Sweden.Objectives: to compare saliva proteome of patients before treatment of head and neck cancer and six months post-treatment with controls. Design: Five dentate patients and five age and gender-matched controls were included. The stimulated salivary secretion rate was determined, and saliva was stored at −80 °C. After thawing, 30 mg of each sample and a reference (aliqouts of all samples) was trypsin digested. The digested peptides were analyzed by mass spectrometry. The relative abundances were transformed to log2 and significant differences determined. Relative abundances of mucins were compared with patient's problems with dry mouth, sticky saliva and swallowing. Data are available via ProteomeXchange with identifier PXD047500. Results: 966 proteins with ≥2 unique peptides were found. Compared with controls, 30 proteins were found in significantly lower relative abundances and 65 in higher at pre-treatment and 38 proteins in significantly lower relative abundances and 34 proteins in higher post-treatment. Regarding proteins from the salivary glands, a significantly lower relative abundance of Cystatins was detected pre-treatment and significantly lower relative abundances of Cystatin, Cysteine-rich secretory protein 3, Lactoperoxidase, Prolactin-inducible protein and Proline-rich protein 4 post-treatment. No clear relation between relative abundance of mucins and dry mouth, sticky saliva and problems with swallowing was detected. Conclusion: Decreases in several salivary gland proteins post cancer treatment might lead to a reduced defense against oral disorders. Knowledge about changes in saliva proteins in connection with oral cancer treatment is important for planning dental care for these patients.http://www.sciencedirect.com/science/article/pii/S2405844024150645Head and neck cancerSaliva proteomeStimulated salivaCystatins |
| spellingShingle | Ulrica Almhöjd Amela Fisic Hülya Cevik-Aras Lisa Tuomi Caterina Finizia Annica Almståhl Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment Heliyon Head and neck cancer Saliva proteome Stimulated saliva Cystatins |
| title | Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment |
| title_full | Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment |
| title_fullStr | Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment |
| title_full_unstemmed | Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment |
| title_short | Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment |
| title_sort | explorative study of stimulated saliva proteome in head and neck cancer patients pre and post treatment |
| topic | Head and neck cancer Saliva proteome Stimulated saliva Cystatins |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024150645 |
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