Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset response
ABSTRACT Respiratory syncytial virus (RSV) causes lower respiratory tract infections in infants and young children, leading to a pathogenesis-associated imbalance in CD4+ T-cell subsets and monocyte subsets. To investigate whether RSV affects the imbalance of CD4+ T-cells through monocytes, we exami...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2025-01-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02073-24 |
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| author | Lianlian Han Danyang Li Conghui Wang Lili Ren Li Guo Jianwei Wang |
| author_facet | Lianlian Han Danyang Li Conghui Wang Lili Ren Li Guo Jianwei Wang |
| author_sort | Lianlian Han |
| collection | DOAJ |
| description | ABSTRACT Respiratory syncytial virus (RSV) causes lower respiratory tract infections in infants and young children, leading to a pathogenesis-associated imbalance in CD4+ T-cell subsets and monocyte subsets. To investigate whether RSV affects the imbalance of CD4+ T-cells through monocytes, we examined the effects of the RSV-infected monocyte subset on CD4+ T-cell subsets, namely, Th1, Th2, Th17, and regulatory T (Treg) subsets, on proliferation in vitro and identified key monocyte-derived cytokines. We found that RSV efficiently infects CD16+ monocytes, but not CD16- monocytes, via cocultures of CD4+ T-cells with RSV-infected CD16+ monocytes, inhibits Treg cell proliferation and increases Th2 cell frequency, suggesting that RSV causes an imbalance in the CD4+ T-cell subset by primarily infecting CD16+ monocytes. Our data also revealed that IL-1β and IL-10 are key cytokines responsible for the activities of RSV-infected CD16+ monocytes. In a mouse model, we found that high-efficiency RSV infection of mouse Ly6C- monocytes, corresponding to CD16+ monocytes in humans, and adoptive transfer of Ly6C- monocytes during RSV infection decreased the Treg frequency in the lungs and aggravated pneumonia. Our data indicate that RSV can increase its pathogenesis through infection of nonclassic monocytes, leading to a CD4+ T-cell imbalance.IMPORTANCEThis study identified a pathogenesis pathway related to the RSV-nonclassic monocyte–IL-1β/IL-10-CD4+ T-cell subset balance, which links the heterogeneity of monocytes to RSV pathogenesis and elucidates a new mechanism by which RSV infection disrupts the balance of CD4+ T cells during RSV infection. These new findings provide potential therapeutic targets for RSV infection. |
| format | Article |
| id | doaj-art-ed737677d5d6459aa12bd787873aeace |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-ed737677d5d6459aa12bd787873aeace2025-01-07T14:05:19ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-01-0113110.1128/spectrum.02073-24Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset responseLianlian Han0Danyang Li1Conghui Wang2Lili Ren3Li Guo4Jianwei Wang5NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaABSTRACT Respiratory syncytial virus (RSV) causes lower respiratory tract infections in infants and young children, leading to a pathogenesis-associated imbalance in CD4+ T-cell subsets and monocyte subsets. To investigate whether RSV affects the imbalance of CD4+ T-cells through monocytes, we examined the effects of the RSV-infected monocyte subset on CD4+ T-cell subsets, namely, Th1, Th2, Th17, and regulatory T (Treg) subsets, on proliferation in vitro and identified key monocyte-derived cytokines. We found that RSV efficiently infects CD16+ monocytes, but not CD16- monocytes, via cocultures of CD4+ T-cells with RSV-infected CD16+ monocytes, inhibits Treg cell proliferation and increases Th2 cell frequency, suggesting that RSV causes an imbalance in the CD4+ T-cell subset by primarily infecting CD16+ monocytes. Our data also revealed that IL-1β and IL-10 are key cytokines responsible for the activities of RSV-infected CD16+ monocytes. In a mouse model, we found that high-efficiency RSV infection of mouse Ly6C- monocytes, corresponding to CD16+ monocytes in humans, and adoptive transfer of Ly6C- monocytes during RSV infection decreased the Treg frequency in the lungs and aggravated pneumonia. Our data indicate that RSV can increase its pathogenesis through infection of nonclassic monocytes, leading to a CD4+ T-cell imbalance.IMPORTANCEThis study identified a pathogenesis pathway related to the RSV-nonclassic monocyte–IL-1β/IL-10-CD4+ T-cell subset balance, which links the heterogeneity of monocytes to RSV pathogenesis and elucidates a new mechanism by which RSV infection disrupts the balance of CD4+ T cells during RSV infection. These new findings provide potential therapeutic targets for RSV infection.https://journals.asm.org/doi/10.1128/spectrum.02073-24RSVnonclassic monocyteTregTh2IL-10IL-1β |
| spellingShingle | Lianlian Han Danyang Li Conghui Wang Lili Ren Li Guo Jianwei Wang Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset response Microbiology Spectrum RSV nonclassic monocyte Treg Th2 IL-10 IL-1β |
| title | Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset response |
| title_full | Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset response |
| title_fullStr | Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset response |
| title_full_unstemmed | Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset response |
| title_short | Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset response |
| title_sort | infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the cd4 t cell subset response |
| topic | RSV nonclassic monocyte Treg Th2 IL-10 IL-1β |
| url | https://journals.asm.org/doi/10.1128/spectrum.02073-24 |
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