How many do we need? Meeting the challenges of studying the microbiome of a cryptic insect in an orchard
The minimal sampling effort required to report the microbiome composition of insect surveyed in natural environment is often based on empirical or logistical constraints. This question was addressed with the white pine cone beetle, Conophthorus coniperda (Schwarz), a devastating insect pest of seed...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1490681/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841558805202075648 |
|---|---|
| author | Apolline Maurin Audrey-Anne Durand Claude Guertin Philippe Constant |
| author_facet | Apolline Maurin Audrey-Anne Durand Claude Guertin Philippe Constant |
| author_sort | Apolline Maurin |
| collection | DOAJ |
| description | The minimal sampling effort required to report the microbiome composition of insect surveyed in natural environment is often based on empirical or logistical constraints. This question was addressed with the white pine cone beetle, Conophthorus coniperda (Schwarz), a devastating insect pest of seed orchards. It attacks and stop the growth of the cones within which it will spend its life, on the ground. To survive, the bark beetle probably interacts with microorganisms involved in alimentation, cold adaptation, and dormancy stage. Deciphering the drivers and benefits of these microorganisms in an orchard first requires methodological development addressing variability of the white pine cone beetle microbiome. The number of insect guts integrated in composite samples prior to DNA extraction and the number of surveyed trees are two features expected to induce variability in recovered microbiome profiles. These two levels of heterogeneity were examined in an orchard experimental area where 12 white pine trees were sampled and 15 cones from each tree were grouped together. For each tree, 2, 3 and 4 insects were selected, their intestinal tract dissected, and the microbiome sequenced. The number of insects caused no significant incidence on the coverage of bacterial and fungal communities’ composition and diversity (p > 0.8). There was more variability among the different trees. A sampling effort including up to 33 trees in an area of 1.1 ha is expected to capture 98% of the microbial diversity in the experimental area. Spatial variability has important implications for future investigations of cryptic insect microbiome. |
| format | Article |
| id | doaj-art-ececa6836a7c4542a3780f50b4dd2187 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-ececa6836a7c4542a3780f50b4dd21872025-01-06T05:13:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14906811490681How many do we need? Meeting the challenges of studying the microbiome of a cryptic insect in an orchardApolline MaurinAudrey-Anne DurandClaude GuertinPhilippe ConstantThe minimal sampling effort required to report the microbiome composition of insect surveyed in natural environment is often based on empirical or logistical constraints. This question was addressed with the white pine cone beetle, Conophthorus coniperda (Schwarz), a devastating insect pest of seed orchards. It attacks and stop the growth of the cones within which it will spend its life, on the ground. To survive, the bark beetle probably interacts with microorganisms involved in alimentation, cold adaptation, and dormancy stage. Deciphering the drivers and benefits of these microorganisms in an orchard first requires methodological development addressing variability of the white pine cone beetle microbiome. The number of insect guts integrated in composite samples prior to DNA extraction and the number of surveyed trees are two features expected to induce variability in recovered microbiome profiles. These two levels of heterogeneity were examined in an orchard experimental area where 12 white pine trees were sampled and 15 cones from each tree were grouped together. For each tree, 2, 3 and 4 insects were selected, their intestinal tract dissected, and the microbiome sequenced. The number of insects caused no significant incidence on the coverage of bacterial and fungal communities’ composition and diversity (p > 0.8). There was more variability among the different trees. A sampling effort including up to 33 trees in an area of 1.1 ha is expected to capture 98% of the microbial diversity in the experimental area. Spatial variability has important implications for future investigations of cryptic insect microbiome.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1490681/fullConophthorus coniperdamicrobiomesampling effortcryptic insectseed orchard’s pest |
| spellingShingle | Apolline Maurin Audrey-Anne Durand Claude Guertin Philippe Constant How many do we need? Meeting the challenges of studying the microbiome of a cryptic insect in an orchard Frontiers in Microbiology Conophthorus coniperda microbiome sampling effort cryptic insect seed orchard’s pest |
| title | How many do we need? Meeting the challenges of studying the microbiome of a cryptic insect in an orchard |
| title_full | How many do we need? Meeting the challenges of studying the microbiome of a cryptic insect in an orchard |
| title_fullStr | How many do we need? Meeting the challenges of studying the microbiome of a cryptic insect in an orchard |
| title_full_unstemmed | How many do we need? Meeting the challenges of studying the microbiome of a cryptic insect in an orchard |
| title_short | How many do we need? Meeting the challenges of studying the microbiome of a cryptic insect in an orchard |
| title_sort | how many do we need meeting the challenges of studying the microbiome of a cryptic insect in an orchard |
| topic | Conophthorus coniperda microbiome sampling effort cryptic insect seed orchard’s pest |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1490681/full |
| work_keys_str_mv | AT apollinemaurin howmanydoweneedmeetingthechallengesofstudyingthemicrobiomeofacrypticinsectinanorchard AT audreyannedurand howmanydoweneedmeetingthechallengesofstudyingthemicrobiomeofacrypticinsectinanorchard AT claudeguertin howmanydoweneedmeetingthechallengesofstudyingthemicrobiomeofacrypticinsectinanorchard AT philippeconstant howmanydoweneedmeetingthechallengesofstudyingthemicrobiomeofacrypticinsectinanorchard |