Deficiency of dltD contributes to increased autolysis in Streptococcus mutans
Streptococcus mutans (S. mutans) is one of the major etiological agents of dental caries. Interfering with the expression of genes associated with cariogenicity of S. mutans is an effective ecological approach for caries prevention. Thus, understanding the role of genes related to cariogenicity in S...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2023.2290221 |
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| author | Jingyun Du Shan Huang Yijun Li Minjing Wu Shuai Chen Ling Zhan Xiaojing Huang |
| author_facet | Jingyun Du Shan Huang Yijun Li Minjing Wu Shuai Chen Ling Zhan Xiaojing Huang |
| author_sort | Jingyun Du |
| collection | DOAJ |
| description | Streptococcus mutans (S. mutans) is one of the major etiological agents of dental caries. Interfering with the expression of genes associated with cariogenicity of S. mutans is an effective ecological approach for caries prevention. Thus, understanding the role of genes related to cariogenicity in S. mutans is important. dltD is a conserved gene in Gram-positive bacteria, which is involved in D-alanylation of lipoteichoic acid to modify cell surface charge. In vivo caries experiments have shown significant decreases in the caries severity in rats infected with S. mutans dltD-deficient strain. Although the impact of dltD on bacterial surface charge and division in other Gram-positive bacteria is known, its role in S. mutans biological functions remains unclear. This study aimed to investigate the biological functions of dltD in S. mutans. We examined growth curves, morphology, autolysis, biofilm formation, and cell membrane properties of S. mutans wild strain and dltD-deficient strain. Our results revealed that dltD plays a key role in the growth and autolysis of S. mutans. dltD deletion increased biofilm autolysis, thereby reducing the number of viable bacteria within the biofilm. Furthermore, dltD may have an epistatic effect on cell division and surface charge. Its regulatory network needs to be further elucidated. This study shed light on therole of dltD in the growth of S. mutans, thereby enhancing our understanding of its function. |
| format | Article |
| id | doaj-art-ecce750bc4a44199b7ad3213c76b1afc |
| institution | Kabale University |
| issn | 2000-2297 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-ecce750bc4a44199b7ad3213c76b1afc2024-11-26T06:52:12ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2023.2290221Deficiency of dltD contributes to increased autolysis in Streptococcus mutansJingyun Du0Shan Huang1Yijun Li2Minjing Wu3Shuai Chen4Ling Zhan5Xiaojing Huang6Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaDivision of Pediatric Dentistry, Department of Orofacial Sciences, Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, CA, USAFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaStreptococcus mutans (S. mutans) is one of the major etiological agents of dental caries. Interfering with the expression of genes associated with cariogenicity of S. mutans is an effective ecological approach for caries prevention. Thus, understanding the role of genes related to cariogenicity in S. mutans is important. dltD is a conserved gene in Gram-positive bacteria, which is involved in D-alanylation of lipoteichoic acid to modify cell surface charge. In vivo caries experiments have shown significant decreases in the caries severity in rats infected with S. mutans dltD-deficient strain. Although the impact of dltD on bacterial surface charge and division in other Gram-positive bacteria is known, its role in S. mutans biological functions remains unclear. This study aimed to investigate the biological functions of dltD in S. mutans. We examined growth curves, morphology, autolysis, biofilm formation, and cell membrane properties of S. mutans wild strain and dltD-deficient strain. Our results revealed that dltD plays a key role in the growth and autolysis of S. mutans. dltD deletion increased biofilm autolysis, thereby reducing the number of viable bacteria within the biofilm. Furthermore, dltD may have an epistatic effect on cell division and surface charge. Its regulatory network needs to be further elucidated. This study shed light on therole of dltD in the growth of S. mutans, thereby enhancing our understanding of its function.https://www.tandfonline.com/doi/10.1080/20002297.2023.2290221streptococcus mutansautolysisdltdmembrane potential |
| spellingShingle | Jingyun Du Shan Huang Yijun Li Minjing Wu Shuai Chen Ling Zhan Xiaojing Huang Deficiency of dltD contributes to increased autolysis in Streptococcus mutans Journal of Oral Microbiology streptococcus mutans autolysis dltd membrane potential |
| title | Deficiency of dltD contributes to increased autolysis in Streptococcus mutans |
| title_full | Deficiency of dltD contributes to increased autolysis in Streptococcus mutans |
| title_fullStr | Deficiency of dltD contributes to increased autolysis in Streptococcus mutans |
| title_full_unstemmed | Deficiency of dltD contributes to increased autolysis in Streptococcus mutans |
| title_short | Deficiency of dltD contributes to increased autolysis in Streptococcus mutans |
| title_sort | deficiency of dltd contributes to increased autolysis in streptococcus mutans |
| topic | streptococcus mutans autolysis dltd membrane potential |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2023.2290221 |
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