Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parameters
Aim: This study aimed to investigate chrysin's molecular, biochemical, and histological effects in an experimental intestinal ischemia/reperfusion (IR) model and to reveal possible protective mechanisms.Material-Methods: 35 Wistar rats were randomly divided into five groups: Control, CHR, IR,...
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Atatürk University
2025-03-01
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| Series: | Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/4320674 |
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| author | Fatih Mehmet Kandemir Ayşe Betül Öztürk Hasan Şimşek Nurhan Akaras |
| author_facet | Fatih Mehmet Kandemir Ayşe Betül Öztürk Hasan Şimşek Nurhan Akaras |
| author_sort | Fatih Mehmet Kandemir |
| collection | DOAJ |
| description | Aim: This study aimed to investigate chrysin's molecular, biochemical, and histological effects in an experimental intestinal ischemia/reperfusion (IR) model and to reveal possible protective mechanisms.Material-Methods: 35 Wistar rats were randomly divided into five groups: Control, CHR, IR, IR+CHR25, IR+CHR50. The IR model was established by inducing ischemia by ligating the superior mesenteric artery for one hour and restoring blood flow for two hours. In the study, MDA and GSH levels were analysed by manual biochemical method; SOD, CAT, GPx activities and NF-κB and NO levels by ELISA method; caspase-3, Beclin-1, LC3A, PERK, ATF-6 mRNA transcription levels by RT-PCR method. In addition, tissue structure was examined histologically. Results: MDA levels were doubled in the IR group and decreased with CHR (p < .05). In addition, CHR increased SOD, CAT, and GPx activities and GSH levels which decreased due to IR (p < .05). Inflammation markers NF-κB and NO were increased; and decreased with CHR (p < .05). Apoptosis marker caspase-3 increased in IR and decreased with CHR (p < .05). Autophagy markers Beclin-1 and LC3A were increased by CHR (p < .05); endoplasmic reticulum stress markers PERK and ATF-6 were increased in IR and decreased by CHR (p < .05). Severe histopathologic changes were observed in the IR and improved with CHR treatment.Conclusion: While IR causes damage to intestinal tissue, the antioxidant and anti-inflammatory properties of CHR have revealed its therapeutic potential against IR injury. |
| format | Article |
| id | doaj-art-eca0276745b04cd09a00d3825832f3c1 |
| institution | Kabale University |
| issn | 2791-8645 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Atatürk University |
| record_format | Article |
| series | Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi |
| spelling | doaj-art-eca0276745b04cd09a00d3825832f3c12025-08-20T03:42:47ZengAtatürk UniversityLaboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi2791-86452025-03-0151495910.62425/jlasp.157490555Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parametersFatih Mehmet Kandemir0https://orcid.org/0000-0002-8490-2479Ayşe Betül Öztürk1https://orcid.org/0000-0001-7773-5978Hasan Şimşek2https://orcid.org/0000-0001-5573-4923Nurhan Akaras3https://orcid.org/0000-0002-8457-9448AKSARAY ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜAKSARAY ÜNİVERSİTESİAKSARAY ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜAKSARAY ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜAim: This study aimed to investigate chrysin's molecular, biochemical, and histological effects in an experimental intestinal ischemia/reperfusion (IR) model and to reveal possible protective mechanisms.Material-Methods: 35 Wistar rats were randomly divided into five groups: Control, CHR, IR, IR+CHR25, IR+CHR50. The IR model was established by inducing ischemia by ligating the superior mesenteric artery for one hour and restoring blood flow for two hours. In the study, MDA and GSH levels were analysed by manual biochemical method; SOD, CAT, GPx activities and NF-κB and NO levels by ELISA method; caspase-3, Beclin-1, LC3A, PERK, ATF-6 mRNA transcription levels by RT-PCR method. In addition, tissue structure was examined histologically. Results: MDA levels were doubled in the IR group and decreased with CHR (p < .05). In addition, CHR increased SOD, CAT, and GPx activities and GSH levels which decreased due to IR (p < .05). Inflammation markers NF-κB and NO were increased; and decreased with CHR (p < .05). Apoptosis marker caspase-3 increased in IR and decreased with CHR (p < .05). Autophagy markers Beclin-1 and LC3A were increased by CHR (p < .05); endoplasmic reticulum stress markers PERK and ATF-6 were increased in IR and decreased by CHR (p < .05). Severe histopathologic changes were observed in the IR and improved with CHR treatment.Conclusion: While IR causes damage to intestinal tissue, the antioxidant and anti-inflammatory properties of CHR have revealed its therapeutic potential against IR injury.https://dergipark.org.tr/tr/download/article-file/4320674apoptosisautophagychrysininflammationintestinalischemia/reperfusionapoptozbağırsakchrysininflamasyoniskemi/reperfüzyonotofaji |
| spellingShingle | Fatih Mehmet Kandemir Ayşe Betül Öztürk Hasan Şimşek Nurhan Akaras Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parameters Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi apoptosis autophagy chrysin inflammation intestinal ischemia/reperfusion apoptoz bağırsak chrysin inflamasyon iskemi/reperfüzyon otofaji |
| title | Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parameters |
| title_full | Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parameters |
| title_fullStr | Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parameters |
| title_full_unstemmed | Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parameters |
| title_short | Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parameters |
| title_sort | investigation of the effects of chrysin on intestinal ischemia reperfusion via apoptotic oxidative stress inflammation and autophagy parameters |
| topic | apoptosis autophagy chrysin inflammation intestinal ischemia/reperfusion apoptoz bağırsak chrysin inflamasyon iskemi/reperfüzyon otofaji |
| url | https://dergipark.org.tr/tr/download/article-file/4320674 |
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