A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis
Abstract Triple-negative breast cancer (TNBC) seriously threatens women’s health, and long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression and play fundamental roles in TNBC. This study aimed to identify lncRNAs that represent effective targets for the early diagnosis...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-83665-0 |
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| author | Ping Ma Shiyao Kang Huimin Li Ming Li Yuan Zhao Hongjun Yuan Jianyu Pang Wenru Tang Miaomiao Sheng |
| author_facet | Ping Ma Shiyao Kang Huimin Li Ming Li Yuan Zhao Hongjun Yuan Jianyu Pang Wenru Tang Miaomiao Sheng |
| author_sort | Ping Ma |
| collection | DOAJ |
| description | Abstract Triple-negative breast cancer (TNBC) seriously threatens women’s health, and long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression and play fundamental roles in TNBC. This study aimed to identify lncRNAs that represent effective targets for the early diagnosis or treatment of TNBC. Here, we utilized the TCGA database to analyze differentially expressed genes, and survival analysis and ROC curve analysis were also performed. Notably, we identified a novel lncRNA, AC112721.1, that is significantly overexpressed in TNBC and is associated with poor overall survival in TNBC patients. Loss- and gain-of-function experiments revealed that AC112721.1 significantly promoted cell proliferation and migration, suppressed cell apoptosis in vitro and inhibited tumorigenesis in vivo. Further study of the mechanisms underlying these effects revealed that AC112721.1 regulates the Ras pathway by directly binding to THBS1 protein and functions as a ceRNA by sponging miR-491-5p to increase the expression of C2CD2L, thereby influencing the progression of TNBC. Our findings indicate that AC112721.1 may represent a new biomarker for evaluating TNBC prognosis and treating TNBC. |
| format | Article |
| id | doaj-art-ec6edd2dc6aa4904ae31b838b6a1df5c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ec6edd2dc6aa4904ae31b838b6a1df5c2025-01-05T12:26:20ZengNature PortfolioScientific Reports2045-23222024-12-0114111410.1038/s41598-024-83665-0A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axisPing Ma0Shiyao Kang1Huimin Li2Ming Li3Yuan Zhao4Hongjun Yuan5Jianyu Pang6Wenru Tang7Miaomiao Sheng8Laboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and TechnologyLaboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and TechnologyLaboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and TechnologyLaboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and TechnologyDepartment of Oncology, Puer People’s HospitalLaboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and TechnologyLaboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and TechnologyLaboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and TechnologyLaboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and TechnologyAbstract Triple-negative breast cancer (TNBC) seriously threatens women’s health, and long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression and play fundamental roles in TNBC. This study aimed to identify lncRNAs that represent effective targets for the early diagnosis or treatment of TNBC. Here, we utilized the TCGA database to analyze differentially expressed genes, and survival analysis and ROC curve analysis were also performed. Notably, we identified a novel lncRNA, AC112721.1, that is significantly overexpressed in TNBC and is associated with poor overall survival in TNBC patients. Loss- and gain-of-function experiments revealed that AC112721.1 significantly promoted cell proliferation and migration, suppressed cell apoptosis in vitro and inhibited tumorigenesis in vivo. Further study of the mechanisms underlying these effects revealed that AC112721.1 regulates the Ras pathway by directly binding to THBS1 protein and functions as a ceRNA by sponging miR-491-5p to increase the expression of C2CD2L, thereby influencing the progression of TNBC. Our findings indicate that AC112721.1 may represent a new biomarker for evaluating TNBC prognosis and treating TNBC.https://doi.org/10.1038/s41598-024-83665-0Triple-negative breast cancerAC112721.1THBS1miR-491-5pC2CD2LProgression |
| spellingShingle | Ping Ma Shiyao Kang Huimin Li Ming Li Yuan Zhao Hongjun Yuan Jianyu Pang Wenru Tang Miaomiao Sheng A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis Scientific Reports Triple-negative breast cancer AC112721.1 THBS1 miR-491-5p C2CD2L Progression |
| title | A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis |
| title_full | A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis |
| title_fullStr | A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis |
| title_full_unstemmed | A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis |
| title_short | A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis |
| title_sort | novel lncrna ac112721 1 promotes the progression of triple negative breast cancer by directly binding to thbs1 and regulating mir 491 5p c2cd2l axis |
| topic | Triple-negative breast cancer AC112721.1 THBS1 miR-491-5p C2CD2L Progression |
| url | https://doi.org/10.1038/s41598-024-83665-0 |
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