In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's disease

Abstract Anti‐amyloidogenic properties of plant pigments betalains as potential nutraceuticals against Alzheimer's disease have been screened using 24 pure molecules. Twenty‐two betalains reduced amyloid aggregation in vitro, eight of them up to 100%, with IC50 values in the micromolar range. A...

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Main Authors: Pedro Martínez‐Rodríguez, Paula Henarejos‐Escudero, Samanta Hernández‐García, Álvaro Sánchez‐Ferrer, Fernando Gandía‐Herrero
Format: Article
Language:English
Published: Wiley 2024-09-01
Series:Food Frontiers
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Online Access:https://doi.org/10.1002/fft2.453
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author Pedro Martínez‐Rodríguez
Paula Henarejos‐Escudero
Samanta Hernández‐García
Álvaro Sánchez‐Ferrer
Fernando Gandía‐Herrero
author_facet Pedro Martínez‐Rodríguez
Paula Henarejos‐Escudero
Samanta Hernández‐García
Álvaro Sánchez‐Ferrer
Fernando Gandía‐Herrero
author_sort Pedro Martínez‐Rodríguez
collection DOAJ
description Abstract Anti‐amyloidogenic properties of plant pigments betalains as potential nutraceuticals against Alzheimer's disease have been screened using 24 pure molecules. Twenty‐two betalains reduced amyloid aggregation in vitro, eight of them up to 100%, with IC50 values in the micromolar range. Atomic force and transmission electron microscopy images showed the typical fibrils associated with Alzheimer's disease and how betalains avoid its formation. Neuroprotection after ingestion was supported by in vivo experiments with Caenorhabditis elegans. Indoline‐betacyanin was the most effective molecule by significantly improving the chemotactic behavior of the CL2355 strain, a model of Alzheimer's disease. Furthermore, in‐depth molecular docking analyses revealed that the pigments interact with the N‐terminal region of the amyloid peptide. This work is the most comprehensive study in the field and provides in vitro, in vivo, and in silico evidence for the use of betalains as nutraceuticals of relevance in the prevention of Alzheimer's disease.
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spelling doaj-art-ec5eef7f545a4ce68357fc80d97c7bf22024-11-17T09:43:58ZengWileyFood Frontiers2643-84292024-09-01552137215410.1002/fft2.453In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's diseasePedro Martínez‐Rodríguez0Paula Henarejos‐Escudero1Samanta Hernández‐García2Álvaro Sánchez‐Ferrer3Fernando Gandía‐Herrero4Department of Biochemistry and Molecular Biology A, Faculty of Biology, Regional Campus of International Excellence, Campus Mare Nostrum University of Murcia Murcia SpainDepartment of Biochemistry and Molecular Biology A, Faculty of Biology, Regional Campus of International Excellence, Campus Mare Nostrum University of Murcia Murcia SpainDepartment of Biochemistry and Molecular Biology A, Faculty of Biology, Regional Campus of International Excellence, Campus Mare Nostrum University of Murcia Murcia SpainDepartment of Biochemistry and Molecular Biology A, Faculty of Biology, Regional Campus of International Excellence, Campus Mare Nostrum University of Murcia Murcia SpainDepartment of Biochemistry and Molecular Biology A, Faculty of Biology, Regional Campus of International Excellence, Campus Mare Nostrum University of Murcia Murcia SpainAbstract Anti‐amyloidogenic properties of plant pigments betalains as potential nutraceuticals against Alzheimer's disease have been screened using 24 pure molecules. Twenty‐two betalains reduced amyloid aggregation in vitro, eight of them up to 100%, with IC50 values in the micromolar range. Atomic force and transmission electron microscopy images showed the typical fibrils associated with Alzheimer's disease and how betalains avoid its formation. Neuroprotection after ingestion was supported by in vivo experiments with Caenorhabditis elegans. Indoline‐betacyanin was the most effective molecule by significantly improving the chemotactic behavior of the CL2355 strain, a model of Alzheimer's disease. Furthermore, in‐depth molecular docking analyses revealed that the pigments interact with the N‐terminal region of the amyloid peptide. This work is the most comprehensive study in the field and provides in vitro, in vivo, and in silico evidence for the use of betalains as nutraceuticals of relevance in the prevention of Alzheimer's disease.https://doi.org/10.1002/fft2.453Alzheimer's disease (AD)betalainsbioactive pigmentsCaenorhabditis elegansneuroprotectionnutraceuticals
spellingShingle Pedro Martínez‐Rodríguez
Paula Henarejos‐Escudero
Samanta Hernández‐García
Álvaro Sánchez‐Ferrer
Fernando Gandía‐Herrero
In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's disease
Food Frontiers
Alzheimer's disease (AD)
betalains
bioactive pigments
Caenorhabditis elegans
neuroprotection
nutraceuticals
title In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's disease
title_full In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's disease
title_fullStr In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's disease
title_full_unstemmed In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's disease
title_short In vitro, in vivo, and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against Alzheimer's disease
title_sort in vitro in vivo and in silico evidence for the use of plant pigments betalains as potential nutraceuticals against alzheimer s disease
topic Alzheimer's disease (AD)
betalains
bioactive pigments
Caenorhabditis elegans
neuroprotection
nutraceuticals
url https://doi.org/10.1002/fft2.453
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