Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acid
Drought conditions severely curtail the ability of plants to accumulate biomass due to the closure of stomata and the decrease of photosynthetic assimilation rate. Additionally, there is a shift in the plant’s metabolic processes toward the production of metabolites that offer protection and aid in...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1385414/full |
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| author | Raed Elferjani Shankar Pahari Raju Soolanayakanahally Krista Ballantyne Eiji Nambara |
| author_facet | Raed Elferjani Shankar Pahari Raju Soolanayakanahally Krista Ballantyne Eiji Nambara |
| author_sort | Raed Elferjani |
| collection | DOAJ |
| description | Drought conditions severely curtail the ability of plants to accumulate biomass due to the closure of stomata and the decrease of photosynthetic assimilation rate. Additionally, there is a shift in the plant’s metabolic processes toward the production of metabolites that offer protection and aid in osmoadaptation, as opposed to those required for development and growth. To limit water loss via non-stomatal transpiration, plants adjust the load and composition of cuticle waxes, which act as an additional barrier. This study investigates the impact of soil water deficit on stomatal and epicuticular water losses, as well as metabolic adjustments in two canola (Brassica napus L.) cultivars—one drought-tolerant and the other drought-sensitive. Specifically, we examined the effect of a drought treatment, which involved reducing water holding capacity to 40%, on the levels of cysteine, sucrose, and abscisic acid (ABA) in the leaves of both cultivars. Next, we looked for potential differences in night, predawn, and early morning transpiration rates and the epicuticular wax load and composition in response to drought. A substantial rise in leaf cysteine was observed in both canola cultivars in response to drought, and a strong correlation was found between cysteine, ABA, and stomatal conductance, indicating that cysteine and sulfur may play a role in controlling stomatal movement during drought stress. Attributes related to CO2 diffusion (stomatal and mesophyll conductance) and photosynthetic capacity were different between the two canola cultivars suggesting a better management of water relations under stress by the drought-tolerant cultivar. Epicuticular waxes were found to adjust in response to drought, acting as an additional barrier against water loss. Surprisingly, both canola cultivars responded similarly to the metabolites (cysteine, sucrose, and ABA) and epicuticular waxes, indicating that they were not reliable stress markers in our test setup. However, the higher level of phenylalanine in the drought-tolerant canola cultivar is suggestive that this amino acid is important for adaptation to drier climates. Furthermore, a multitrait genotype-ideotype distance index (MGIDI) revealed the likely role of aspartic acid in sustaining nitrogen and carbon for immediate photosynthetic resumption after drought episodes. In conclusion, leveraging amino acid knowledge in agriculture can enhance crop yield and bolster resistance to environmental challenges. |
| format | Article |
| id | doaj-art-ec16b4d764d042588fb88381e7c1d24c |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-ec16b4d764d042588fb88381e7c1d24c2025-01-08T11:18:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.13854141385414Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acidRaed Elferjani0Shankar Pahari1Raju Soolanayakanahally2Krista Ballantyne3Eiji Nambara4Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK, CanadaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK, CanadaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK, CanadaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK, CanadaDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, CanadaDrought conditions severely curtail the ability of plants to accumulate biomass due to the closure of stomata and the decrease of photosynthetic assimilation rate. Additionally, there is a shift in the plant’s metabolic processes toward the production of metabolites that offer protection and aid in osmoadaptation, as opposed to those required for development and growth. To limit water loss via non-stomatal transpiration, plants adjust the load and composition of cuticle waxes, which act as an additional barrier. This study investigates the impact of soil water deficit on stomatal and epicuticular water losses, as well as metabolic adjustments in two canola (Brassica napus L.) cultivars—one drought-tolerant and the other drought-sensitive. Specifically, we examined the effect of a drought treatment, which involved reducing water holding capacity to 40%, on the levels of cysteine, sucrose, and abscisic acid (ABA) in the leaves of both cultivars. Next, we looked for potential differences in night, predawn, and early morning transpiration rates and the epicuticular wax load and composition in response to drought. A substantial rise in leaf cysteine was observed in both canola cultivars in response to drought, and a strong correlation was found between cysteine, ABA, and stomatal conductance, indicating that cysteine and sulfur may play a role in controlling stomatal movement during drought stress. Attributes related to CO2 diffusion (stomatal and mesophyll conductance) and photosynthetic capacity were different between the two canola cultivars suggesting a better management of water relations under stress by the drought-tolerant cultivar. Epicuticular waxes were found to adjust in response to drought, acting as an additional barrier against water loss. Surprisingly, both canola cultivars responded similarly to the metabolites (cysteine, sucrose, and ABA) and epicuticular waxes, indicating that they were not reliable stress markers in our test setup. However, the higher level of phenylalanine in the drought-tolerant canola cultivar is suggestive that this amino acid is important for adaptation to drier climates. Furthermore, a multitrait genotype-ideotype distance index (MGIDI) revealed the likely role of aspartic acid in sustaining nitrogen and carbon for immediate photosynthetic resumption after drought episodes. In conclusion, leveraging amino acid knowledge in agriculture can enhance crop yield and bolster resistance to environmental challenges.https://www.frontiersin.org/articles/10.3389/fpls.2024.1385414/fullBrassica napusdroughtamino acidscysteinephenylalanineaspartic acid |
| spellingShingle | Raed Elferjani Shankar Pahari Raju Soolanayakanahally Krista Ballantyne Eiji Nambara Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acid Frontiers in Plant Science Brassica napus drought amino acids cysteine phenylalanine aspartic acid |
| title | Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acid |
| title_full | Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acid |
| title_fullStr | Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acid |
| title_full_unstemmed | Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acid |
| title_short | Drought induced metabolic shifts and water loss mechanisms in canola: role of cysteine, phenylalanine and aspartic acid |
| title_sort | drought induced metabolic shifts and water loss mechanisms in canola role of cysteine phenylalanine and aspartic acid |
| topic | Brassica napus drought amino acids cysteine phenylalanine aspartic acid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1385414/full |
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