Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis
This study aims to determine the applicability of tofu wastewater as a medium for yeast growth and to determine the optimal growth time of yeast to produce β-glucans, as well as the yield and characteristics of β-glucans extract. Pichia norvegensis and Candida tropicalis cell walls were extracted fo...
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Elsevier
2025-01-01
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| Series: | Current Research in Green and Sustainable Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666086525000013 |
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| author | Deidita Nafisa Wahyudi Gemilang Lara Utama Andri Frediansyah |
| author_facet | Deidita Nafisa Wahyudi Gemilang Lara Utama Andri Frediansyah |
| author_sort | Deidita Nafisa Wahyudi |
| collection | DOAJ |
| description | This study aims to determine the applicability of tofu wastewater as a medium for yeast growth and to determine the optimal growth time of yeast to produce β-glucans, as well as the yield and characteristics of β-glucans extract. Pichia norvegensis and Candida tropicalis cell walls were extracted for β-glucan. FTIR, solid-state NMR, and SEM were utilized to investigate purity, structural differences, and antioxidant activity. Candida tropicalis (BCT) yielded 3.20 % crude β-glucan, while Pichia norvegiensis NYI (BPN) yielded 2.10 %. FTIR showed β-glucan impurities in mannoprotein-chitosan combinations. BPN and BCT have β-glucan bands at 1075, 1041, and 894 cm−1. The impurity of both samples was confirmed by NMR spectra, which showed that the broad signal of BPN and BCT chemical shift at 4.803–4.587 ppm and at 4.764–4.541 ppm, respectively, corresponded to l β-1,3-glucosidic and β-1,6-glucosidic linkage, with some unusual anomeric resonance at 5.094–5.024 ppm by SEM, BPN and BCT were porous, sheet-like, and fibrous. BPN had 20.07 % DPPH radical scavenging activity, while BCT had 36.01 %. |
| format | Article |
| id | doaj-art-ec15b1aa89354d36ace48be694291c80 |
| institution | Kabale University |
| issn | 2666-0865 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Green and Sustainable Chemistry |
| spelling | doaj-art-ec15b1aa89354d36ace48be694291c802025-01-12T05:25:45ZengElsevierCurrent Research in Green and Sustainable Chemistry2666-08652025-01-0110100445Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalisDeidita Nafisa Wahyudi0Gemilang Lara Utama1Andri Frediansyah2Magister Program of of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, University of Padjadjaran, Jatinangor, 45363, Indonesia; Corresponding author.Magister Program of of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, University of Padjadjaran, Jatinangor, 45363, Indonesia; Centre for Environment and Sustainability Science, University of Padjadjaran, Bandung, 40134, Indonesia; Corresponding author. Magister program of of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, University of Padjadjaran, jatinangor, 45363, Indonesia.Research Group of Biotechnology and Microbial Metabolites for Food, National Research and Innovation Agency (BRIN), Yogyakarta, Indonesia; Research Center for Food Technology and Processing, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Yogyakarta, IndonesiaThis study aims to determine the applicability of tofu wastewater as a medium for yeast growth and to determine the optimal growth time of yeast to produce β-glucans, as well as the yield and characteristics of β-glucans extract. Pichia norvegensis and Candida tropicalis cell walls were extracted for β-glucan. FTIR, solid-state NMR, and SEM were utilized to investigate purity, structural differences, and antioxidant activity. Candida tropicalis (BCT) yielded 3.20 % crude β-glucan, while Pichia norvegiensis NYI (BPN) yielded 2.10 %. FTIR showed β-glucan impurities in mannoprotein-chitosan combinations. BPN and BCT have β-glucan bands at 1075, 1041, and 894 cm−1. The impurity of both samples was confirmed by NMR spectra, which showed that the broad signal of BPN and BCT chemical shift at 4.803–4.587 ppm and at 4.764–4.541 ppm, respectively, corresponded to l β-1,3-glucosidic and β-1,6-glucosidic linkage, with some unusual anomeric resonance at 5.094–5.024 ppm by SEM, BPN and BCT were porous, sheet-like, and fibrous. BPN had 20.07 % DPPH radical scavenging activity, while BCT had 36.01 %.http://www.sciencedirect.com/science/article/pii/S2666086525000013PichiaCandidaβ-glucanFTIRNMRSEM |
| spellingShingle | Deidita Nafisa Wahyudi Gemilang Lara Utama Andri Frediansyah Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis Current Research in Green and Sustainable Chemistry Pichia Candida β-glucan FTIR NMR SEM |
| title | Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis |
| title_full | Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis |
| title_fullStr | Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis |
| title_full_unstemmed | Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis |
| title_short | Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis |
| title_sort | tofu wastewater recovery for β glucan production by pichia norvegensis and candida tropicalis |
| topic | Pichia Candida β-glucan FTIR NMR SEM |
| url | http://www.sciencedirect.com/science/article/pii/S2666086525000013 |
| work_keys_str_mv | AT deiditanafisawahyudi tofuwastewaterrecoveryforbglucanproductionbypichianorvegensisandcandidatropicalis AT gemilanglarautama tofuwastewaterrecoveryforbglucanproductionbypichianorvegensisandcandidatropicalis AT andrifrediansyah tofuwastewaterrecoveryforbglucanproductionbypichianorvegensisandcandidatropicalis |