Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining
Summary: cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulati...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724006476 |
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| author | Beatriz Maio Nagore Elu Cristina Martinez-Gonzalez Emily K. Osterweil Susana R. Louros |
| author_facet | Beatriz Maio Nagore Elu Cristina Martinez-Gonzalez Emily K. Osterweil Susana R. Louros |
| author_sort | Beatriz Maio |
| collection | DOAJ |
| description | Summary: cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus. We then detail procedures for image acquisition and analysis.For complete details on the use and execution of this protocol, please refer to Louros et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-ebb9e9df8d8f43a3ac7bcebecb88ef4d |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-ebb9e9df8d8f43a3ac7bcebecb88ef4d2024-12-13T11:03:51ZengElsevierSTAR Protocols2666-16672024-12-0154103482Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostainingBeatriz Maio0Nagore Elu1Cristina Martinez-Gonzalez2Emily K. Osterweil3Susana R. Louros4Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK; Simons Initiative for the Developing Brain, Patrick Wild Centre, University of Edinburgh, Edinburgh, UKCentre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK; Simons Initiative for the Developing Brain, Patrick Wild Centre, University of Edinburgh, Edinburgh, UKCentre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK; Simons Initiative for the Developing Brain, Patrick Wild Centre, University of Edinburgh, Edinburgh, UKCentre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK; Simons Initiative for the Developing Brain, Patrick Wild Centre, University of Edinburgh, Edinburgh, UK; Rosamund Stone Zander Translational Neuroscience Center, F. M. Kirby Center, Department of Neurology, Harvard Medical School, Boston Children’s Hospital, Boston, MA, USA; Corresponding authorCentre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK; Simons Initiative for the Developing Brain, Patrick Wild Centre, University of Edinburgh, Edinburgh, UK; Corresponding authorSummary: cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus. We then detail procedures for image acquisition and analysis.For complete details on the use and execution of this protocol, please refer to Louros et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724006476microscopymodel organismsneuroscience |
| spellingShingle | Beatriz Maio Nagore Elu Cristina Martinez-Gonzalez Emily K. Osterweil Susana R. Louros Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining STAR Protocols microscopy model organisms neuroscience |
| title | Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining |
| title_full | Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining |
| title_fullStr | Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining |
| title_full_unstemmed | Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining |
| title_short | Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining |
| title_sort | protocol for identifying sound activated neurons in the inferior colliculus by cfos immunostaining |
| topic | microscopy model organisms neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724006476 |
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