Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation
Abstract Background The pivotal intent of this study was to investigate the medicinal potential of synthesized nanotransferosomes utilizing Clerodendrum serratum L. hydroalcoholic extract (CS-NTs). Three batches, CS-NTs 25, CS-NTs 50, and CS-NTs 100, were formulated using the thin-film hydration app...
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SpringerOpen
2025-01-01
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| Series: | Future Journal of Pharmaceutical Sciences |
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| Online Access: | https://doi.org/10.1186/s43094-024-00755-6 |
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| author | Somnath Devidas Bhinge Sayali Jadhav Pranali Lade Mangesh Anil Bhutkar Shailendra Gurav Namdeo Jadhav Abhinandan Patil Neeraj Upmanyu |
| author_facet | Somnath Devidas Bhinge Sayali Jadhav Pranali Lade Mangesh Anil Bhutkar Shailendra Gurav Namdeo Jadhav Abhinandan Patil Neeraj Upmanyu |
| author_sort | Somnath Devidas Bhinge |
| collection | DOAJ |
| description | Abstract Background The pivotal intent of this study was to investigate the medicinal potential of synthesized nanotransferosomes utilizing Clerodendrum serratum L. hydroalcoholic extract (CS-NTs). Three batches, CS-NTs 25, CS-NTs 50, and CS-NTs 100, were formulated using the thin-film hydration approach with varying concentrations of phospholipids (soya phosphatidylcholine) at 25, 50, and 100 mg, respectively, to evaluate their efficacy against skin cancer cell lines. Several analytical approaches, such as UV/Vis spectroscopy, XRD spectra, FTIR, TEM, SEM, particle size distribution, and zeta potential analysis, were employed to assess the synthesized CS-NTs. Results Experimental results revealed that CS-NTs exhibited a circular shape, with average vesicle sizes approximately measured at 463.6 ± 100.5 nm, 265.5 ± 61.6 nm, and 409.6 ± 106.2 nm for CS-NTs 25, CS-NTs 50, and CS-NTs 100, respectively. Notably, biologically synthesized CS-NTs 25 offer potential anticancer benefits against B10F16 (skin cancer cell line) cells, exhibiting no signs of adverse effects. The heightened biological potential of CS-NTs can be ascribed to their unique characteristics at the nanoscale, characterized by an average vesicle size and lower polydispersity value, which facilitate augmented responsiveness and interactions with biological systems. Conclusion CS-NTs 25 formulated through the biogenic process using C. serratum hydroalcoholic plant root extract hold significant potential for anticancer activity. Graphical abstract |
| format | Article |
| id | doaj-art-ea9f34a21051448cb595933b26d9abfe |
| institution | Kabale University |
| issn | 2314-7253 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
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| series | Future Journal of Pharmaceutical Sciences |
| spelling | doaj-art-ea9f34a21051448cb595933b26d9abfe2025-01-12T12:11:13ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532025-01-0111111710.1186/s43094-024-00755-6Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluationSomnath Devidas Bhinge0Sayali Jadhav1Pranali Lade2Mangesh Anil Bhutkar3Shailendra Gurav4Namdeo Jadhav5Abhinandan Patil6Neeraj Upmanyu7Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, KasegaonRajarambapu College of Pharmacy, Kasegaon, Shivaji UniversityRajarambapu College of Pharmacy, Kasegaon, Shivaji UniversityRajarambapu College of Pharmacy, Kasegaon, Shivaji UniversityDepartment of Pharmacognosy, Goa College of Pharmacy, Panaji, Goa UniversityDean, Krishna Institute of Pharmacy, Krishna Vishwa Vidyapeeth KaradDepartment of Pharmaceutics, D. Y. Patil College of Pharmacy, D Y Patil Education Society (Deemed to be University)SAGE UniversityAbstract Background The pivotal intent of this study was to investigate the medicinal potential of synthesized nanotransferosomes utilizing Clerodendrum serratum L. hydroalcoholic extract (CS-NTs). Three batches, CS-NTs 25, CS-NTs 50, and CS-NTs 100, were formulated using the thin-film hydration approach with varying concentrations of phospholipids (soya phosphatidylcholine) at 25, 50, and 100 mg, respectively, to evaluate their efficacy against skin cancer cell lines. Several analytical approaches, such as UV/Vis spectroscopy, XRD spectra, FTIR, TEM, SEM, particle size distribution, and zeta potential analysis, were employed to assess the synthesized CS-NTs. Results Experimental results revealed that CS-NTs exhibited a circular shape, with average vesicle sizes approximately measured at 463.6 ± 100.5 nm, 265.5 ± 61.6 nm, and 409.6 ± 106.2 nm for CS-NTs 25, CS-NTs 50, and CS-NTs 100, respectively. Notably, biologically synthesized CS-NTs 25 offer potential anticancer benefits against B10F16 (skin cancer cell line) cells, exhibiting no signs of adverse effects. The heightened biological potential of CS-NTs can be ascribed to their unique characteristics at the nanoscale, characterized by an average vesicle size and lower polydispersity value, which facilitate augmented responsiveness and interactions with biological systems. Conclusion CS-NTs 25 formulated through the biogenic process using C. serratum hydroalcoholic plant root extract hold significant potential for anticancer activity. Graphical abstracthttps://doi.org/10.1186/s43094-024-00755-6Clerodendrum serratum L.Thin-film hydration methodNanotransferosomesSkin cancerB10F16 |
| spellingShingle | Somnath Devidas Bhinge Sayali Jadhav Pranali Lade Mangesh Anil Bhutkar Shailendra Gurav Namdeo Jadhav Abhinandan Patil Neeraj Upmanyu Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation Future Journal of Pharmaceutical Sciences Clerodendrum serratum L. Thin-film hydration method Nanotransferosomes Skin cancer B10F16 |
| title | Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation |
| title_full | Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation |
| title_fullStr | Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation |
| title_full_unstemmed | Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation |
| title_short | Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation |
| title_sort | biogenic nanotransferosomal vesicular system of clerodendrum serratum l for skin cancer therapy formulation characterization and efficacy evaluation |
| topic | Clerodendrum serratum L. Thin-film hydration method Nanotransferosomes Skin cancer B10F16 |
| url | https://doi.org/10.1186/s43094-024-00755-6 |
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