Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in glioma
Increasing evidence indicates that long noncoding RNAs play important roles in development and progression of various cancers. Zinc finger antisense 1 is a novel long noncoding RNA whose clinical significance, biological function, and underlying mechanism are still undetermined in glioma. In this st...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-03-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317695022 |
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| author | Qiao-Li Lv Shu-Hui Chen Xue Zhang Bao Sun Lei Hu Qiang Qu Yuan-Tao Huang Gui-Hua Wang Yan-Ling Liu Ying-Ying Zhang Hong-Hao Zhou |
| author_facet | Qiao-Li Lv Shu-Hui Chen Xue Zhang Bao Sun Lei Hu Qiang Qu Yuan-Tao Huang Gui-Hua Wang Yan-Ling Liu Ying-Ying Zhang Hong-Hao Zhou |
| author_sort | Qiao-Li Lv |
| collection | DOAJ |
| description | Increasing evidence indicates that long noncoding RNAs play important roles in development and progression of various cancers. Zinc finger antisense 1 is a novel long noncoding RNA whose clinical significance, biological function, and underlying mechanism are still undetermined in glioma. In this study, we reported that zinc finger antisense 1 expression was markedly upregulated in glioma and tightly correlated with clinical stage. Moreover, patients with high zinc finger antisense 1 expression had shorter survival. Multivariate Cox regression analysis provided a clue that, probably, zinc finger antisense 1 level could serve as an independent prognostic factor for glioma. Functionally, zinc finger antisense 1 acted as an oncogene in glioma because its knockdown could promote apoptosis and significantly inhibit cell proliferation, migration, and invasion. Furthermore, zinc finger antisense 1 silencing could result in cell cycle arrest at the G0/G1 phase and correspondingly decrease the percentage of S phase cells in both U87 and U251 cell lines. Moreover, it was found that silenced zinc finger antisense 1 could impair migration and invasion by inhibiting the epithelial–mesenchymal transition through reducing the expression of MMP2, MMP9, N-cadherin, Integrin β1, ZEB1, Twist, and Snail as well as increasing E-cadherin level in glioma. Taken together, our data identified that zinc finger antisense 1 might act as a valuable prognostic biomarker and potential therapeutic target for glioma. |
| format | Article |
| id | doaj-art-ea9c881cc14f45f0b03bf811d6676f78 |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-ea9c881cc14f45f0b03bf811d6676f782025-08-20T03:39:17ZengSAGE PublishingTumor Biology1423-03802017-03-013910.1177/1010428317695022Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in gliomaQiao-Li Lv0Shu-Hui Chen1Xue Zhang2Bao Sun3Lei Hu4Qiang Qu5Yuan-Tao Huang6Gui-Hua Wang7Yan-Ling Liu8Ying-Ying Zhang9Hong-Hao Zhou10Hunan Key Laboratory of Pharmacogenetics, Institute of Clinical Pharmacology, Central South University, Changsha, P.R. ChinaDepartment of Oncology, Changsha Central Hospital, Changsha, P.R. ChinaHunan Key Laboratory of Pharmacogenetics, Institute of Clinical Pharmacology, Central South University, Changsha, P.R. ChinaHunan Key Laboratory of Pharmacogenetics, Institute of Clinical Pharmacology, Central South University, Changsha, P.R. ChinaHunan Key Laboratory of Pharmacogenetics, Institute of Clinical Pharmacology, Central South University, Changsha, P.R. ChinaDepartment of Pharmacy, Xiangya Hospital, Central South University, Changsha, P.R. ChinaDepartment of Neurology, The Brain Hospital of Hunan Province, Changsha, P.R. ChinaDepartment of Oncology, Changsha Central Hospital, Changsha, P.R. ChinaHunan Key Laboratory of Pharmacogenetics, Institute of Clinical Pharmacology, Central South University, Changsha, P.R. ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, P.R. ChinaHunan Key Laboratory of Pharmacogenetics, Institute of Clinical Pharmacology, Central South University, Changsha, P.R. ChinaIncreasing evidence indicates that long noncoding RNAs play important roles in development and progression of various cancers. Zinc finger antisense 1 is a novel long noncoding RNA whose clinical significance, biological function, and underlying mechanism are still undetermined in glioma. In this study, we reported that zinc finger antisense 1 expression was markedly upregulated in glioma and tightly correlated with clinical stage. Moreover, patients with high zinc finger antisense 1 expression had shorter survival. Multivariate Cox regression analysis provided a clue that, probably, zinc finger antisense 1 level could serve as an independent prognostic factor for glioma. Functionally, zinc finger antisense 1 acted as an oncogene in glioma because its knockdown could promote apoptosis and significantly inhibit cell proliferation, migration, and invasion. Furthermore, zinc finger antisense 1 silencing could result in cell cycle arrest at the G0/G1 phase and correspondingly decrease the percentage of S phase cells in both U87 and U251 cell lines. Moreover, it was found that silenced zinc finger antisense 1 could impair migration and invasion by inhibiting the epithelial–mesenchymal transition through reducing the expression of MMP2, MMP9, N-cadherin, Integrin β1, ZEB1, Twist, and Snail as well as increasing E-cadherin level in glioma. Taken together, our data identified that zinc finger antisense 1 might act as a valuable prognostic biomarker and potential therapeutic target for glioma.https://doi.org/10.1177/1010428317695022 |
| spellingShingle | Qiao-Li Lv Shu-Hui Chen Xue Zhang Bao Sun Lei Hu Qiang Qu Yuan-Tao Huang Gui-Hua Wang Yan-Ling Liu Ying-Ying Zhang Hong-Hao Zhou Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in glioma Tumor Biology |
| title | Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in glioma |
| title_full | Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in glioma |
| title_fullStr | Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in glioma |
| title_full_unstemmed | Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in glioma |
| title_short | Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in glioma |
| title_sort | upregulation of long noncoding rna zinc finger antisense 1 enhances epithelial mesenchymal transition in vitro and predicts poor prognosis in glioma |
| url | https://doi.org/10.1177/1010428317695022 |
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