Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces
Abstract Osseointegration is essential for successful implant treatment. However, the underlying molecular mechanisms remain unclear. In this study, we focused on decorin (DCN), which was hypothesized to be present in the proteoglycan (PG) layer at the interface between bone and the titanium oxide (...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06849-0 |
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| author | Hisanobu Kamio Kazuto Okabe Masaki Honda Kensuke Kuroda Shuhei Tsuchiya |
| author_facet | Hisanobu Kamio Kazuto Okabe Masaki Honda Kensuke Kuroda Shuhei Tsuchiya |
| author_sort | Hisanobu Kamio |
| collection | DOAJ |
| description | Abstract Osseointegration is essential for successful implant treatment. However, the underlying molecular mechanisms remain unclear. In this study, we focused on decorin (DCN), which was hypothesized to be present in the proteoglycan (PG) layer at the interface between bone and the titanium oxide (TiOx) surface. We utilized DCN RNA interference in human bone marrow mesenchymal stem cells (hBMSCs) to investigate its effects on PG layer formation, proliferation, initial adhesion, cell extension, osteogenic capacity, fibrotic markers, and immunotolerance to TiOx in vitro. After 14 days of cultivation, we observed no PG layer was detected, and the osteogenic capacity was suppressed in DCN-depleted hBMSCs. Furthermore, the conditioned medium upregulated the expression of M1 macrophage markers in human macrophages. These results suggest that endogenous DCN plays a crucial role in PG layer formation and that the PG layer alters inflammation around Ti materials. Graphical Abstract |
| format | Article |
| id | doaj-art-ea61b19e8f784b50a91f5dbd38d8cd98 |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-ea61b19e8f784b50a91f5dbd38d8cd982025-01-12T12:06:18ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382025-01-0136111310.1007/s10856-024-06849-0Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfacesHisanobu Kamio0Kazuto Okabe1Masaki Honda2Kensuke Kuroda3Shuhei Tsuchiya4Department of Dental Anesthesiology, Division of Oral and Maxillofacial Surgery and Oral Medicine, Hiroshima University HospitalDepartment of Oral and Maxillofacial Surgery, Nagoya City University Graduate School of Medical SciencesDepartment of Oral Anatomy, School of Dentistry, Aichi Gakuin UniversityInstitutes of Innovation for Future Society, Nagoya UniversityDepartment of Oral and Maxillofacial Surgery, Nagoya City University Graduate School of Medical SciencesAbstract Osseointegration is essential for successful implant treatment. However, the underlying molecular mechanisms remain unclear. In this study, we focused on decorin (DCN), which was hypothesized to be present in the proteoglycan (PG) layer at the interface between bone and the titanium oxide (TiOx) surface. We utilized DCN RNA interference in human bone marrow mesenchymal stem cells (hBMSCs) to investigate its effects on PG layer formation, proliferation, initial adhesion, cell extension, osteogenic capacity, fibrotic markers, and immunotolerance to TiOx in vitro. After 14 days of cultivation, we observed no PG layer was detected, and the osteogenic capacity was suppressed in DCN-depleted hBMSCs. Furthermore, the conditioned medium upregulated the expression of M1 macrophage markers in human macrophages. These results suggest that endogenous DCN plays a crucial role in PG layer formation and that the PG layer alters inflammation around Ti materials. Graphical Abstracthttps://doi.org/10.1007/s10856-024-06849-0 |
| spellingShingle | Hisanobu Kamio Kazuto Okabe Masaki Honda Kensuke Kuroda Shuhei Tsuchiya Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces Journal of Materials Science: Materials in Medicine |
| title | Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces |
| title_full | Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces |
| title_fullStr | Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces |
| title_full_unstemmed | Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces |
| title_short | Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces |
| title_sort | knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro inflammatory environment on titanium oxide surfaces |
| url | https://doi.org/10.1007/s10856-024-06849-0 |
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