Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter
Background: IgG antibodies bound to erythrocytes may be involved in various disease processes. Identification of these antibodies and antigen typing of patients with a positive direct antiglobulin (DAT) test is relevant both for transfusion therapy and follow-up of patients during pregnancy. We have...
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| Main Authors: | , |
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| Format: | Article |
| Language: | Danish |
| Published: |
NITO - Bioingeniørfaglig institutt
2024-10-01
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| Series: | Bioingeniøren |
| Subjects: | |
| Online Access: | https://forskning.bioingenioren.no/originalartikkel/sammenligning-av-metoder-for-eluering-av-igg-antistoff-bundet-til-erytrocytter/116303 |
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| Summary: | Background: IgG antibodies bound to erythrocytes may be involved in various disease processes. Identification of these antibodies and antigen typing of patients with a positive direct antiglobulin (DAT) test is relevant both for transfusion therapy and follow-up of patients during pregnancy. We have compared elution efficiency and concordance of results for various CE (Communauté Européenne) and IVD (In vitro Diagnostic) labelled methods and heat elution.
Method: Elution methods for stripping of DAT-positive erythrocytes and for detection of antibodies in eluate were compared. McNemars test was used to compare the effectiveness of the methods. P-values < 0,05 were considered statistically significant and Holm-Bonferronis method for multiple comparisons was used. The proportion of matching results between the methods was calculated as a percentage.
Results: We demonstrated significant differences in diagnostic sensitivity between the different methods. Concordance of results between methods ranged from 64 to 90 %. In antigen typing of DAT-negative erythrocytes after antibody stripping, both false negative and false positive results were observed.
Conclusion: Methods for elution of IgG antibodies from erythrocytes should be included in routine investigations for certain problems. Different methods do not provide consistent results for all problems; therefore, alternative methods should be available.
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| ISSN: | 0801-6828 1890-1875 |