Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models
c-Jun is often found to be overexpressed in various cancers, so this gene might be a target for cancer therapy. Newcastle disease virus (NDV) is recognized for its oncolytic properties and potential as a cancer virotherapy agent, with various mechanisms reported to trigger cancer cell death. This st...
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| Format: | Article |
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Universitas Airlangga
2025-01-01
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| Series: | Media Kedokteran Hewan |
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| Online Access: | https://e-journal.unair.ac.id/MKH/article/view/62141 |
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| author | Fransiska Gratia Sonita Marson Palagan Senopati Sewoyo I Nyoman Mantik Astawa Anak Agung Ayu Mirah Adi Ida Bagus Kade Suardana Ida Bagus Oka Winaya I Ketut Berata |
| author_facet | Fransiska Gratia Sonita Marson Palagan Senopati Sewoyo I Nyoman Mantik Astawa Anak Agung Ayu Mirah Adi Ida Bagus Kade Suardana Ida Bagus Oka Winaya I Ketut Berata |
| author_sort | Fransiska Gratia Sonita Marson |
| collection | DOAJ |
| description | c-Jun is often found to be overexpressed in various cancers, so this gene might be a target for cancer therapy. Newcastle disease virus (NDV) is recognized for its oncolytic properties and potential as a cancer virotherapy agent, with various mechanisms reported to trigger cancer cell death. This study aimed to assess the c-Jun expression in rat mammary cancer models. Rat mammary cancer models were categorized into two treatment groups: the control group (C) and the virotherapy group (V). Group C was administered with 0.5 cc of sterile PBS, while group V received 7 log 2 HAU per 0.5 cc of the Indonesian NDV field isolate Tabanan-1/ARP/2017 intratumorally. The treatment was carried out for four days in a row. Two weeks after treatment, all rats were humanely euthanized, and mammary cancer tissues were excised for further examination. Mammary cancer tissues were examined histopathologically and analyzed using immunohistochemistry to determine intranuclear c-Jun expression, quantified by the H-Score. The results demonstrated that NDV significantly reduced c-Jun expression. It can be inferred that NDV Tabanan-1/ARP/2017 holds potential as a mammary cancer therapy agent by reducing c-Jun expression. This finding is considered novel, as there have been no previous reports of decreased c-Jun expression following virotherapy with NDV. |
| format | Article |
| id | doaj-art-ea30f53b34a648d1a9b756d2680ffb40 |
| institution | Kabale University |
| issn | 0215-8930 2775-975X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Media Kedokteran Hewan |
| spelling | doaj-art-ea30f53b34a648d1a9b756d2680ffb402025-01-10T11:23:48ZengUniversitas AirlanggaMedia Kedokteran Hewan0215-89302775-975X2025-01-01361132010.20473/mkh.v36i1.2025.13-2060308Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer ModelsFransiska Gratia Sonita Marson0https://orcid.org/0009-0007-2002-2038Palagan Senopati Sewoyo1https://orcid.org/0000-0002-8713-7725I Nyoman Mantik Astawa2https://orcid.org/0000-0002-0957-0902Anak Agung Ayu Mirah Adi3https://orcid.org/0000-0002-0983-252XIda Bagus Kade Suardana4https://orcid.org/0009-0008-5415-9988Ida Bagus Oka Winaya5https://orcid.org/0000-0003-3051-0901I Ketut Berata6https://orcid.org/0000-0003-3217-2598Department of Pathobiology, Faculty of Veterinary Medicine, Udayana UniversityDepartment of Pathobiology, Faculty of Veterinary Medicine, Udayana University Department of Pathobiology, Faculty of Veterinary Medicine, Udayana UniversityDepartment of Pathobiology, Faculty of Veterinary Medicine, Udayana UniversityDepartment of Pathobiology, Faculty of Veterinary Medicine, Udayana UniversityDepartment of Pathobiology, Faculty of Veterinary Medicine, Udayana UniversityDepartment of Pathobiology, Faculty of Veterinary Medicine, Udayana Universityc-Jun is often found to be overexpressed in various cancers, so this gene might be a target for cancer therapy. Newcastle disease virus (NDV) is recognized for its oncolytic properties and potential as a cancer virotherapy agent, with various mechanisms reported to trigger cancer cell death. This study aimed to assess the c-Jun expression in rat mammary cancer models. Rat mammary cancer models were categorized into two treatment groups: the control group (C) and the virotherapy group (V). Group C was administered with 0.5 cc of sterile PBS, while group V received 7 log 2 HAU per 0.5 cc of the Indonesian NDV field isolate Tabanan-1/ARP/2017 intratumorally. The treatment was carried out for four days in a row. Two weeks after treatment, all rats were humanely euthanized, and mammary cancer tissues were excised for further examination. Mammary cancer tissues were examined histopathologically and analyzed using immunohistochemistry to determine intranuclear c-Jun expression, quantified by the H-Score. The results demonstrated that NDV significantly reduced c-Jun expression. It can be inferred that NDV Tabanan-1/ARP/2017 holds potential as a mammary cancer therapy agent by reducing c-Jun expression. This finding is considered novel, as there have been no previous reports of decreased c-Jun expression following virotherapy with NDV.https://e-journal.unair.ac.id/MKH/article/view/62141c-junmammary cancernewcastle disease virusvirotherapy |
| spellingShingle | Fransiska Gratia Sonita Marson Palagan Senopati Sewoyo I Nyoman Mantik Astawa Anak Agung Ayu Mirah Adi Ida Bagus Kade Suardana Ida Bagus Oka Winaya I Ketut Berata Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models Media Kedokteran Hewan c-jun mammary cancer newcastle disease virus virotherapy |
| title | Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models |
| title_full | Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models |
| title_fullStr | Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models |
| title_full_unstemmed | Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models |
| title_short | Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models |
| title_sort | indonesian newcastle disease virus field isolate reduces c jun expression in rat mammary cancer models |
| topic | c-jun mammary cancer newcastle disease virus virotherapy |
| url | https://e-journal.unair.ac.id/MKH/article/view/62141 |
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