Isolation and identification of enterococcus durans and enterococcus hirae from mastitic cattle milks
<b>Aim:</b> In this study, it was aimed to isolation and identification of Enterococcus durans and Enterococcus hirae which are frequently mixed with each other due to very high 16S rRNA similarity from mastitis cow milk samples and also to determine antibiotic resistance profiles of iso...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1239 |
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| Summary: | <b>Aim:</b> In this study, it was aimed to isolation and identification of Enterococcus
durans and Enterococcus hirae which are frequently mixed
with each other due to very high 16S rRNA similarity from mastitis
cow milk samples and also to determine antibiotic resistance profiles
of isolates.<p>
<b>Materials and Methods: </b>The study material was consisted of 620 clinical
or subclinical mastitis milk samples. Enterococci isolation was
performed using conventional microbological methods and selective
agars. Identifications based on genus and species were also confirmed
by polymerase chain reaction (PCR). Resistance against to eight antimicrobial
agents was investigated by the disk diffusion method.<p>
<b>Results:</b> In the study 15.2% (94/620) Enterococcus spp. isolated;
9.6% (9/94) of them which were Enterococcus hirae and 5.3% (5/94)
were Enterococcus durans. A total of 14 isolates were found to be susceptible
to ampicillin, penicillin, vancomycin, ciprofloxacin and chloramphenicol.
57.1% (8/14), 42.9% (6/14) and 21.4% (3/14) were
resistant to tetracycline, erythromycin and gentamycin, respectively.<p>
<b>Conclusion:</b> It has been concluded that the polymerase chain reaction
using species-specific primers is useful for the identification of frequently
isolated enterococci from mastitic cattle milk samples. More attention
should be paid to enterococci species other than Enterococcus
faecalis and Enterococcus faecium and the monitoring of antimicrobial
resistance should not be restricted to these two main species. Studies
on the identification of virulence factors and antibiotic resistance
genes of these two enterococci are thought to contribute to a better
understanding of the pathogenesis of these microorganisms. |
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| ISSN: | 1309-6958 2146-1953 |