Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani Population
Background: Klebsiella pneumoniae is an opportunistic pathogen and is responsible for life threatening hospital acquired infections. The multidrug resistant (MDR) strains are emerging and limit therapeutic options. This study characterizes local clinical strains of K. pneumoniae phenotypically and g...
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524002121 |
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| author | Rabia Ilyas Sidrah Asghar Ayaz Ahmed |
| author_facet | Rabia Ilyas Sidrah Asghar Ayaz Ahmed |
| author_sort | Rabia Ilyas |
| collection | DOAJ |
| description | Background: Klebsiella pneumoniae is an opportunistic pathogen and is responsible for life threatening hospital acquired infections. The multidrug resistant (MDR) strains are emerging and limit therapeutic options. This study characterizes local clinical strains of K. pneumoniae phenotypically and genotypically and evaluate clonal relatedness among isolates. Methods: The strains were collected from hospitals of Karachi and determined their virulence or antimicrobial profile via disc diffusion method, MIC determination and antibiotic resistant genes, biofilm forming genes and for genetically similarity ERIC PCR was performed. The intranasal pneumoniae model was developed against the most virulent strain to mimick the infection in mice. Results: The clinical strains of K. pneumoniae (n=113) were identified through 16srRNA and rpoB gene. Overall,89.3% were MDR strains and isolated from urine, blood, pus, tracheal aspirate, pleural, and cerebral spinal fluid. Antibiography showed that the isolates were resistant to class beta lactams, aminoglycoside, quinolones, and tetracycline and also showed resistance upto last resort drugs. The genotypic characterization found outbreak genes BlaNDM, BlaOXA-48, BlaTEM, BlaSHV. The virulence profile showed 97% are biofilm formers with their virulence genes. ERIC PCR showed heterogenous profile among isolates. The progressive intranasal pneumoniae model was developed through blood strains (3.2×107) at evaluate pathogenicity through clinical score, bacterial load and histopathology. Conclusion: The results of this study clear indicate the presence of highly virulent strains within the population and showed resistant to last resort drug options. Therefore, this study suggests the strict monitoring of MDR isolates of K. pneumoniae and formulate AMR policy to tackle their further. |
| format | Article |
| id | doaj-art-e9fe986ec8c54d0b8d4f75d21c681979 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-e9fe986ec8c54d0b8d4f75d21c6819792024-12-27T04:08:18ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-01391112Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani PopulationRabia Ilyas0Sidrah Asghar1Ayaz Ahmed2Dr. Panjwani Center for Molecular Medicine and Drug Research, ICCBS, University of Karachi, PakistanDr. Panjwani Center for Molecular Medicine and Drug Research, ICCBS, University of Karachi, PakistanDr. Panjwani Center for Molecular Medicine and Drug Research, ICCBS, University of Karachi, PakistanBackground: Klebsiella pneumoniae is an opportunistic pathogen and is responsible for life threatening hospital acquired infections. The multidrug resistant (MDR) strains are emerging and limit therapeutic options. This study characterizes local clinical strains of K. pneumoniae phenotypically and genotypically and evaluate clonal relatedness among isolates. Methods: The strains were collected from hospitals of Karachi and determined their virulence or antimicrobial profile via disc diffusion method, MIC determination and antibiotic resistant genes, biofilm forming genes and for genetically similarity ERIC PCR was performed. The intranasal pneumoniae model was developed against the most virulent strain to mimick the infection in mice. Results: The clinical strains of K. pneumoniae (n=113) were identified through 16srRNA and rpoB gene. Overall,89.3% were MDR strains and isolated from urine, blood, pus, tracheal aspirate, pleural, and cerebral spinal fluid. Antibiography showed that the isolates were resistant to class beta lactams, aminoglycoside, quinolones, and tetracycline and also showed resistance upto last resort drugs. The genotypic characterization found outbreak genes BlaNDM, BlaOXA-48, BlaTEM, BlaSHV. The virulence profile showed 97% are biofilm formers with their virulence genes. ERIC PCR showed heterogenous profile among isolates. The progressive intranasal pneumoniae model was developed through blood strains (3.2×107) at evaluate pathogenicity through clinical score, bacterial load and histopathology. Conclusion: The results of this study clear indicate the presence of highly virulent strains within the population and showed resistant to last resort drug options. Therefore, this study suggests the strict monitoring of MDR isolates of K. pneumoniae and formulate AMR policy to tackle their further.http://www.sciencedirect.com/science/article/pii/S2213716524002121Multidrug resistant(MDR)Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC PCR)Klebsiella pneumoniaeMinimum Inhibitory Concentration(MIC) |
| spellingShingle | Rabia Ilyas Sidrah Asghar Ayaz Ahmed Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani Population Journal of Global Antimicrobial Resistance Multidrug resistant(MDR) Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC PCR) Klebsiella pneumoniae Minimum Inhibitory Concentration(MIC) |
| title | Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani Population |
| title_full | Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani Population |
| title_fullStr | Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani Population |
| title_full_unstemmed | Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani Population |
| title_short | Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani Population |
| title_sort | virulence characterization of antimicrobial resistant clinical isolates of klebsiella pneumoniae circulating in pakistani population |
| topic | Multidrug resistant(MDR) Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC PCR) Klebsiella pneumoniae Minimum Inhibitory Concentration(MIC) |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524002121 |
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