Virulence Characterization of Antimicrobial Resistant Clinical Isolates of Klebsiella pneumoniae circulating in Pakistani Population
Background: Klebsiella pneumoniae is an opportunistic pathogen and is responsible for life threatening hospital acquired infections. The multidrug resistant (MDR) strains are emerging and limit therapeutic options. This study characterizes local clinical strains of K. pneumoniae phenotypically and g...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524002121 |
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| Summary: | Background: Klebsiella pneumoniae is an opportunistic pathogen and is responsible for life threatening hospital acquired infections. The multidrug resistant (MDR) strains are emerging and limit therapeutic options. This study characterizes local clinical strains of K. pneumoniae phenotypically and genotypically and evaluate clonal relatedness among isolates. Methods: The strains were collected from hospitals of Karachi and determined their virulence or antimicrobial profile via disc diffusion method, MIC determination and antibiotic resistant genes, biofilm forming genes and for genetically similarity ERIC PCR was performed. The intranasal pneumoniae model was developed against the most virulent strain to mimick the infection in mice. Results: The clinical strains of K. pneumoniae (n=113) were identified through 16srRNA and rpoB gene. Overall,89.3% were MDR strains and isolated from urine, blood, pus, tracheal aspirate, pleural, and cerebral spinal fluid. Antibiography showed that the isolates were resistant to class beta lactams, aminoglycoside, quinolones, and tetracycline and also showed resistance upto last resort drugs. The genotypic characterization found outbreak genes BlaNDM, BlaOXA-48, BlaTEM, BlaSHV. The virulence profile showed 97% are biofilm formers with their virulence genes. ERIC PCR showed heterogenous profile among isolates. The progressive intranasal pneumoniae model was developed through blood strains (3.2×107) at evaluate pathogenicity through clinical score, bacterial load and histopathology. Conclusion: The results of this study clear indicate the presence of highly virulent strains within the population and showed resistant to last resort drug options. Therefore, this study suggests the strict monitoring of MDR isolates of K. pneumoniae and formulate AMR policy to tackle their further. |
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| ISSN: | 2213-7165 |