Characteristics of Sulfur-oxidizing Bacteria Communities in Wastewater Treatment Processes Analyzed Based on Electron Donor and Acceptor Availability

In this study, we analyzed changes in the community structure of sulfur-oxidizing bacteria (SOB) in a wastewater treatment process handling urban sewage over a 1-year period. Facultative anaerobic SOB, which can use nitrate as an electron acceptor, were predominant over obligate aerobic SOB in the a...

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Main Authors: Ryoki Asano, Atsushi Hayakawa
Format: Article
Language:English
Published: Japan Society on Water Environment 2025-01-01
Series:Journal of Water and Environment Technology
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Online Access: https://www.jstage.jst.go.jp/article/jwet/23/2/23_24-116/_pdf
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author Ryoki Asano
Atsushi Hayakawa
author_facet Ryoki Asano
Atsushi Hayakawa
author_sort Ryoki Asano
collection DOAJ
description In this study, we analyzed changes in the community structure of sulfur-oxidizing bacteria (SOB) in a wastewater treatment process handling urban sewage over a 1-year period. Facultative anaerobic SOB, which can use nitrate as an electron acceptor, were predominant over obligate aerobic SOB in the activated sludge tank and clarifier/settler throughout the investigation period, with mixotrophic SOB being the dominant group. Facultative anaerobic SOB were predominant in most wastewater treatment facilities included in our comparative analysis, confirming observations in the investigated facility. Although the constituent species of SOB in the activated sludge tank and clarifier/settler remained consistent over the investigation period, the proportion of SOB relative to all analyzed sequences fluctuated between 0.91% and 6.20%, with periodic increases and decreases. Sulfuritalea hydrogenivorans, which was the predominant SOB in the activated sludge tank at the investigated facility, exhibited maximum and minimum 11.1- and 6.8-fold differences in abundance between the open and closed tanks, respectively. We conclude that sulfur oxidation by SOB during wastewater treatment can contribute to denitrification under anaerobic conditions. Furthermore, the population of predominant SOB species in the investigated wastewater treatment facility exhibited cyclical fluctuation.
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spelling doaj-art-e9fb62f734434d40b82987dc322e15b12025-08-20T03:45:59ZengJapan Society on Water EnvironmentJournal of Water and Environment Technology1348-21652025-01-0123210711910.2965/jwet.24-11610.2965/jwet.24-116Characteristics of Sulfur-oxidizing Bacteria Communities in Wastewater Treatment Processes Analyzed Based on Electron Donor and Acceptor AvailabilityRyoki Asano0Atsushi Hayakawa1 Department of Agro-Food Science, Faculty of Agro-Food Science, Niigata Agro-Food University, Tainai, Japan Department of Biological Environment, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan In this study, we analyzed changes in the community structure of sulfur-oxidizing bacteria (SOB) in a wastewater treatment process handling urban sewage over a 1-year period. Facultative anaerobic SOB, which can use nitrate as an electron acceptor, were predominant over obligate aerobic SOB in the activated sludge tank and clarifier/settler throughout the investigation period, with mixotrophic SOB being the dominant group. Facultative anaerobic SOB were predominant in most wastewater treatment facilities included in our comparative analysis, confirming observations in the investigated facility. Although the constituent species of SOB in the activated sludge tank and clarifier/settler remained consistent over the investigation period, the proportion of SOB relative to all analyzed sequences fluctuated between 0.91% and 6.20%, with periodic increases and decreases. Sulfuritalea hydrogenivorans, which was the predominant SOB in the activated sludge tank at the investigated facility, exhibited maximum and minimum 11.1- and 6.8-fold differences in abundance between the open and closed tanks, respectively. We conclude that sulfur oxidation by SOB during wastewater treatment can contribute to denitrification under anaerobic conditions. Furthermore, the population of predominant SOB species in the investigated wastewater treatment facility exhibited cyclical fluctuation. https://www.jstage.jst.go.jp/article/jwet/23/2/23_24-116/_pdf bacterial communitydna analysissulfur-oxidizing bacteriawastewater treatment
spellingShingle Ryoki Asano
Atsushi Hayakawa
Characteristics of Sulfur-oxidizing Bacteria Communities in Wastewater Treatment Processes Analyzed Based on Electron Donor and Acceptor Availability
Journal of Water and Environment Technology
bacterial community
dna analysis
sulfur-oxidizing bacteria
wastewater treatment
title Characteristics of Sulfur-oxidizing Bacteria Communities in Wastewater Treatment Processes Analyzed Based on Electron Donor and Acceptor Availability
title_full Characteristics of Sulfur-oxidizing Bacteria Communities in Wastewater Treatment Processes Analyzed Based on Electron Donor and Acceptor Availability
title_fullStr Characteristics of Sulfur-oxidizing Bacteria Communities in Wastewater Treatment Processes Analyzed Based on Electron Donor and Acceptor Availability
title_full_unstemmed Characteristics of Sulfur-oxidizing Bacteria Communities in Wastewater Treatment Processes Analyzed Based on Electron Donor and Acceptor Availability
title_short Characteristics of Sulfur-oxidizing Bacteria Communities in Wastewater Treatment Processes Analyzed Based on Electron Donor and Acceptor Availability
title_sort characteristics of sulfur oxidizing bacteria communities in wastewater treatment processes analyzed based on electron donor and acceptor availability
topic bacterial community
dna analysis
sulfur-oxidizing bacteria
wastewater treatment
url https://www.jstage.jst.go.jp/article/jwet/23/2/23_24-116/_pdf
work_keys_str_mv AT ryokiasano characteristicsofsulfuroxidizingbacteriacommunitiesinwastewatertreatmentprocessesanalyzedbasedonelectrondonorandacceptoravailability
AT atsushihayakawa characteristicsofsulfuroxidizingbacteriacommunitiesinwastewatertreatmentprocessesanalyzedbasedonelectrondonorandacceptoravailability