Potential of heat shock protein inducers in mitigating benzo[α]pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responses
Abstract Benzo[α]pyrene (BαP), a polycyclic aromatic hydrocarbon (PAH), is a significant contaminant in the environment, which accumulates and is toxic to invertebrates and fish. The present study aimed to determine the acute toxicity of sublethal concentrations of BαP in the presence of the heat sh...
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2024-12-01
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| Series: | Journal of the World Aquaculture Society |
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| Online Access: | https://doi.org/10.1111/jwas.13092 |
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| author | Leila Vahdatiraad Behrooz Heidari Sevda Zarei Tooraj Sohrabi Hossein Ghafouri |
| author_facet | Leila Vahdatiraad Behrooz Heidari Sevda Zarei Tooraj Sohrabi Hossein Ghafouri |
| author_sort | Leila Vahdatiraad |
| collection | DOAJ |
| description | Abstract Benzo[α]pyrene (BαP), a polycyclic aromatic hydrocarbon (PAH), is a significant contaminant in the environment, which accumulates and is toxic to invertebrates and fish. The present study aimed to determine the acute toxicity of sublethal concentrations of BαP in the presence of the heat shock protein (HSP) inducer (HSPi) on CYP450 activity, HSP70 gene expression, antioxidant levels, immunological alterations, and AChE activity in stellate sturgeon fingerlings. Two‐hundred and forty fish were exposed to 100 mg L−1 of Nopal endurance (HSPi) for 4 h. In the next step, the fish were exposed to BαP concentrations equivalent to 25%, 50%, and 75% of the 96‐h LC50 values. Sampling was carried out on the first, third, and sixth days of the experiment, and the samples were analyzed using two‐way analysis of variance (ANOVA), cluster analysis (CA), and principal component analysis (PCA). The CYP450 activity significantly increased under BαP treatments. Higher expression of the HSP70 gene was observed in the higher concentration of HSPi + BαP treatments. The lowest AChE activity was observed in BαP treatments. However, the activity of superoxide dismutase, catalase, and total antioxidant activity enzymes and immunological responses (lysozyme, IgM, and C3) increased by using HSPi. It can be concluded that the HSP inducer significantly decreased BαP toxicity and resulted in more resistance to stress situations in sturgeon fingerlings. |
| format | Article |
| id | doaj-art-e9bd9ac7985f44d68e76cc4c1aba8029 |
| institution | Kabale University |
| issn | 0893-8849 1749-7345 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
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| series | Journal of the World Aquaculture Society |
| spelling | doaj-art-e9bd9ac7985f44d68e76cc4c1aba80292024-12-03T08:12:32ZengWileyJournal of the World Aquaculture Society0893-88491749-73452024-12-01556n/an/a10.1111/jwas.13092Potential of heat shock protein inducers in mitigating benzo[α]pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responsesLeila Vahdatiraad0Behrooz Heidari1Sevda Zarei2Tooraj Sohrabi3Hossein Ghafouri4Department of Biology, Faculty of Science University of Guilan Rasht IranDepartment of Biology, Faculty of Science University of Guilan Rasht IranDepartment of Biology, Faculty of Science University of Guilan Rasht IranInternational Caspian Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO) Rasht IranDepartment of Biology, Faculty of Science University of Guilan Rasht IranAbstract Benzo[α]pyrene (BαP), a polycyclic aromatic hydrocarbon (PAH), is a significant contaminant in the environment, which accumulates and is toxic to invertebrates and fish. The present study aimed to determine the acute toxicity of sublethal concentrations of BαP in the presence of the heat shock protein (HSP) inducer (HSPi) on CYP450 activity, HSP70 gene expression, antioxidant levels, immunological alterations, and AChE activity in stellate sturgeon fingerlings. Two‐hundred and forty fish were exposed to 100 mg L−1 of Nopal endurance (HSPi) for 4 h. In the next step, the fish were exposed to BαP concentrations equivalent to 25%, 50%, and 75% of the 96‐h LC50 values. Sampling was carried out on the first, third, and sixth days of the experiment, and the samples were analyzed using two‐way analysis of variance (ANOVA), cluster analysis (CA), and principal component analysis (PCA). The CYP450 activity significantly increased under BαP treatments. Higher expression of the HSP70 gene was observed in the higher concentration of HSPi + BαP treatments. The lowest AChE activity was observed in BαP treatments. However, the activity of superoxide dismutase, catalase, and total antioxidant activity enzymes and immunological responses (lysozyme, IgM, and C3) increased by using HSPi. It can be concluded that the HSP inducer significantly decreased BαP toxicity and resulted in more resistance to stress situations in sturgeon fingerlings.https://doi.org/10.1111/jwas.13092Acipenser stellatusbenzo[α]pyreneheat shock protein inducer |
| spellingShingle | Leila Vahdatiraad Behrooz Heidari Sevda Zarei Tooraj Sohrabi Hossein Ghafouri Potential of heat shock protein inducers in mitigating benzo[α]pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responses Journal of the World Aquaculture Society Acipenser stellatus benzo[α]pyrene heat shock protein inducer |
| title | Potential of heat shock protein inducers in mitigating benzo[α]pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responses |
| title_full | Potential of heat shock protein inducers in mitigating benzo[α]pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responses |
| title_fullStr | Potential of heat shock protein inducers in mitigating benzo[α]pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responses |
| title_full_unstemmed | Potential of heat shock protein inducers in mitigating benzo[α]pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responses |
| title_short | Potential of heat shock protein inducers in mitigating benzo[α]pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responses |
| title_sort | potential of heat shock protein inducers in mitigating benzo α pyrene toxicity in stellate sturgeon fingerlings acipenser stellatus assessing the effects on cyp450 and ache activity hsp70 expression antioxidant levels cortisol and immunological responses |
| topic | Acipenser stellatus benzo[α]pyrene heat shock protein inducer |
| url | https://doi.org/10.1111/jwas.13092 |
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