The association of rs25487 of the XRCC1 gene and rs13181 of the ERCC2 gene polymorphisms with the ovarian cancer risk

Ovarian cancer (OC) is the most lethal gynecological cancer worldwide. DNA damage plays an important role in cancer development, and the proteins encoded by XRCC1 and ERCC2 are important components of the DNA repair system. This study aimed to examine the relationship between the rs25487 XRCC1 and...

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Main Authors: Tatiana Zavarykina, Maria Kapralova, Polina Lomskova, Aleksandra Asaturova, Grigory Khabas, Lyailya Kayumova, Dmitry Khodyrev, Irina Pronina, Maya Sannikova, Svetlana Khokhlova
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Language:English
Published: Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2024-12-01
Series:Biomolecules & Biomedicine
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Online Access:https://www.bjbms.org/ojs/index.php/bjbms/article/view/11314
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author Tatiana Zavarykina
Maria Kapralova
Polina Lomskova
Aleksandra Asaturova
Grigory Khabas
Lyailya Kayumova
Dmitry Khodyrev
Irina Pronina
Maya Sannikova
Svetlana Khokhlova
author_facet Tatiana Zavarykina
Maria Kapralova
Polina Lomskova
Aleksandra Asaturova
Grigory Khabas
Lyailya Kayumova
Dmitry Khodyrev
Irina Pronina
Maya Sannikova
Svetlana Khokhlova
author_sort Tatiana Zavarykina
collection DOAJ
description Ovarian cancer (OC) is the most lethal gynecological cancer worldwide. DNA damage plays an important role in cancer development, and the proteins encoded by XRCC1 and ERCC2 are important components of the DNA repair system. This study aimed to examine the relationship between the rs25487 XRCC1 and rs13181 ERCC2 polymorphisms and the risk of OC development in women from the Moscow region. DNA was isolated from the blood of 129 healthy donors and tissues and blood samples from 125 patients with OC and studied using real-time PCR. An increase in odds ratios (OR) was obtained for OC tissue and blood for both T (OR = 1.46, 95% confidence interval [CI] = 1.22–1.76, P = 0.00005), and for T/T of rs25487 XRCC1. The most significant OR values were found for the T/T genotype using the codominant model (OR = 2.11, 95% CI = 1.44–3.07, P = 0.00006) and dominant model (OR = 3.13, 95% CI = 1.44–6.79, P = 0.0025) for the pooled blood and tissue groups. For rs13181 ERCC2, differences were observed for the T/G genotype in OC tissues (OR = 0.69, 95% CI = 0.51–0.92, P = 0.011) in the codominant model. In this study, the association of allele T and genotypes of rs25487 XRCC1 and T/G of rs13181 ERCC2 with OC was shown. Our results indicate that these polymorphisms may be involved in the pathogenesis of OC and are promising for further studies on therapeutic applications in OC.
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spelling doaj-art-e9bb03f32acd46edbd8df9ead19ef1dd2024-12-19T16:30:57ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2024-12-0110.17305/bb.2024.11314The association of rs25487 of the XRCC1 gene and rs13181 of the ERCC2 gene polymorphisms with the ovarian cancer riskTatiana Zavarykina0https://orcid.org/0000-0002-5993-6351Maria Kapralova1https://orcid.org/0000-0003-3010-3994Polina Lomskova2Aleksandra Asaturova3https://orcid.org/0000-0001-8739-5209Grigory Khabas4Lyailya Kayumova5https://orcid.org/0000-0003-0301-737XDmitry Khodyrev6Irina Pronina7https://orcid.org/0000-0002-0423-7801Maya Sannikova8Svetlana Khokhlova9https://orcid.org/0000-0002-4597-172XN.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Moscow, Russia; "B.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology", Ministry of Health of the Russian Federation, Moscow, RussiaN.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Moscow, RussiaN.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Moscow, Russia"B.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology", Ministry of Health of the Russian Federation, Moscow, Russia"B.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology", Ministry of Health of the Russian Federation, Moscow, RussiaSechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaFederal Scientific and Clinical Center of Specialized Types of Medical Care and Medical Technologies, Federal Medical and Biological Agency of the Russian Federation, Moscow, RussiaN.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Moscow, Russia; Institute of General Pathology and Pathophysiology, Moscow, Russia"B.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology", Ministry of Health of the Russian Federation, Moscow, Russia; Yaroslav-the-Wise Novgorod State University, Novgorod, Russia"B.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology", Ministry of Health of the Russian Federation, Moscow, Russia Ovarian cancer (OC) is the most lethal gynecological cancer worldwide. DNA damage plays an important role in cancer development, and the proteins encoded by XRCC1 and ERCC2 are important components of the DNA repair system. This study aimed to examine the relationship between the rs25487 XRCC1 and rs13181 ERCC2 polymorphisms and the risk of OC development in women from the Moscow region. DNA was isolated from the blood of 129 healthy donors and tissues and blood samples from 125 patients with OC and studied using real-time PCR. An increase in odds ratios (OR) was obtained for OC tissue and blood for both T (OR = 1.46, 95% confidence interval [CI] = 1.22–1.76, P = 0.00005), and for T/T of rs25487 XRCC1. The most significant OR values were found for the T/T genotype using the codominant model (OR = 2.11, 95% CI = 1.44–3.07, P = 0.00006) and dominant model (OR = 3.13, 95% CI = 1.44–6.79, P = 0.0025) for the pooled blood and tissue groups. For rs13181 ERCC2, differences were observed for the T/G genotype in OC tissues (OR = 0.69, 95% CI = 0.51–0.92, P = 0.011) in the codominant model. In this study, the association of allele T and genotypes of rs25487 XRCC1 and T/G of rs13181 ERCC2 with OC was shown. Our results indicate that these polymorphisms may be involved in the pathogenesis of OC and are promising for further studies on therapeutic applications in OC. https://www.bjbms.org/ojs/index.php/bjbms/article/view/11314ovarian cancerOCDNA repairXRCC1 geneERCC2 genepolymorphism
spellingShingle Tatiana Zavarykina
Maria Kapralova
Polina Lomskova
Aleksandra Asaturova
Grigory Khabas
Lyailya Kayumova
Dmitry Khodyrev
Irina Pronina
Maya Sannikova
Svetlana Khokhlova
The association of rs25487 of the XRCC1 gene and rs13181 of the ERCC2 gene polymorphisms with the ovarian cancer risk
Biomolecules & Biomedicine
ovarian cancer
OC
DNA repair
XRCC1 gene
ERCC2 gene
polymorphism
title The association of rs25487 of the XRCC1 gene and rs13181 of the ERCC2 gene polymorphisms with the ovarian cancer risk
title_full The association of rs25487 of the XRCC1 gene and rs13181 of the ERCC2 gene polymorphisms with the ovarian cancer risk
title_fullStr The association of rs25487 of the XRCC1 gene and rs13181 of the ERCC2 gene polymorphisms with the ovarian cancer risk
title_full_unstemmed The association of rs25487 of the XRCC1 gene and rs13181 of the ERCC2 gene polymorphisms with the ovarian cancer risk
title_short The association of rs25487 of the XRCC1 gene and rs13181 of the ERCC2 gene polymorphisms with the ovarian cancer risk
title_sort association of rs25487 of the xrcc1 gene and rs13181 of the ercc2 gene polymorphisms with the ovarian cancer risk
topic ovarian cancer
OC
DNA repair
XRCC1 gene
ERCC2 gene
polymorphism
url https://www.bjbms.org/ojs/index.php/bjbms/article/view/11314
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