Visual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification
Abstract Development of rapid and simple detection method holds great significance for the prevention and control of Staphylococcus aureus. Herein, the SELEX method was utilized to screen new aptamers for S. aureus. The double aptamers combined recognition target strategy were adopted to enhance spe...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-01-01
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| Series: | Journal of Analytical Science and Technology |
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| Online Access: | https://doi.org/10.1186/s40543-024-00473-x |
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| _version_ | 1841544629835530240 |
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| author | Bingying Li Meng Yu Siying Ma Yingying Huang Nenghua Zhang Shaoyun Chen |
| author_facet | Bingying Li Meng Yu Siying Ma Yingying Huang Nenghua Zhang Shaoyun Chen |
| author_sort | Bingying Li |
| collection | DOAJ |
| description | Abstract Development of rapid and simple detection method holds great significance for the prevention and control of Staphylococcus aureus. Herein, the SELEX method was utilized to screen new aptamers for S. aureus. The double aptamers combined recognition target strategy were adopted to enhance specificity, and magnetic bead target enrichment and the rolling circle amplification strategy were employed to improve sensitivity, thereby achieving a highly specific and highly sensitive visual detection of S. aureus. This method can specifically identify S. aureus and has high detection sensitivity, with a minimum detection limit of 10 CFU/mL. It also shows good repeatability, with a total time consumption of less than 9 h and can be used for the visual detection of actual samples. |
| format | Article |
| id | doaj-art-e9b6e4e7e7974928b1e83f49493d42be |
| institution | Kabale University |
| issn | 2093-3371 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj-art-e9b6e4e7e7974928b1e83f49493d42be2025-01-12T12:27:10ZengSpringerOpenJournal of Analytical Science and Technology2093-33712025-01-011611810.1186/s40543-024-00473-xVisual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplificationBingying Li0Meng Yu1Siying Ma2Yingying Huang3Nenghua Zhang4Shaoyun Chen5School of Life Sciences, Zhejiang Chinese Medical UniversitySchool of Life Sciences, Zhejiang Chinese Medical UniversitySchool of Life Sciences, Zhejiang Chinese Medical UniversitySchool of Medicine, Zhejiang UniversityClinical Laboratory, Jiaxing Hospital of Traditional Chinese MedicineSchool of Life Sciences, Zhejiang Chinese Medical UniversityAbstract Development of rapid and simple detection method holds great significance for the prevention and control of Staphylococcus aureus. Herein, the SELEX method was utilized to screen new aptamers for S. aureus. The double aptamers combined recognition target strategy were adopted to enhance specificity, and magnetic bead target enrichment and the rolling circle amplification strategy were employed to improve sensitivity, thereby achieving a highly specific and highly sensitive visual detection of S. aureus. This method can specifically identify S. aureus and has high detection sensitivity, with a minimum detection limit of 10 CFU/mL. It also shows good repeatability, with a total time consumption of less than 9 h and can be used for the visual detection of actual samples.https://doi.org/10.1186/s40543-024-00473-xStaphylococcus aureus (S. aureus)Magnetic bead target enrichmentRolling circle amplification (RCA)AptamerVisual detection |
| spellingShingle | Bingying Li Meng Yu Siying Ma Yingying Huang Nenghua Zhang Shaoyun Chen Visual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification Journal of Analytical Science and Technology Staphylococcus aureus (S. aureus) Magnetic bead target enrichment Rolling circle amplification (RCA) Aptamer Visual detection |
| title | Visual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification |
| title_full | Visual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification |
| title_fullStr | Visual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification |
| title_full_unstemmed | Visual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification |
| title_short | Visual detection of Staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification |
| title_sort | visual detection of staphylococcus aureus based on magnetic bead target enrichment and rolling circle amplification |
| topic | Staphylococcus aureus (S. aureus) Magnetic bead target enrichment Rolling circle amplification (RCA) Aptamer Visual detection |
| url | https://doi.org/10.1186/s40543-024-00473-x |
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