Detection of bovine coronavirus (bcov) infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animals
<b>Aim: </b>It was aimed to reveal the antigenic and serological prevalence of bovine coronavirus (BCoV) in cattle with clinically respiratory infection and to evaluate the relationship between virus shedding and serological status of animals in this study.<p> <b>Materials an...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2023-12-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1437 |
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| author | Meltem Menekse Atilla Simsek |
| author_facet | Meltem Menekse Atilla Simsek |
| author_sort | Meltem Menekse |
| collection | DOAJ |
| description | <b>Aim: </b>It was aimed to reveal the antigenic and serological prevalence of
bovine coronavirus (BCoV) in cattle with clinically respiratory infection and
to evaluate the relationship between virus shedding and serological status of
animals in this study.<p>
<b>Materials and Methods:</b> In the study, nasal swab samples were collected
from a total of 92 cattle of different gender between 0 and 2 years of age
with clinical respiratory symptoms. These samples were subjected to RT-PCR
using specific primer pairs. Furthermore, serum samples were also collected
from the same animals and evaluated for BCoV-specific antibodies through an
indirect ELISA method.<p>
<b>Results:</b> Ten (10.87%) swap samples were defined as positive for BCoV using
RT-PCR. The seropositivity rate was 93.48% (86/92) in cattle by ELISA and
90% (9/10) in virus-shedding animals with nasal swab samples.<p>
<b>Conclusion:</b> The fact that 93.47% seropositivity was detected in unvaccinated
animals against coronavirus in this study indicates that the animals were
exposed to BCoV through colostrum or at a certain period of their lives.
However the fact that 9 out of 10 animals with BCoV detected in swab samples
also have BCoV-specific antibodies casts doubt on the efficacy of these
antibodies in protecting against respiratory BCoV infections. We concluded
that more detailed studies are needed to reveal the role of antibodies in
respiratory coronavirus infections. |
| format | Article |
| id | doaj-art-e99a42caad0b4d68a9f56dbfec31ff88 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-e99a42caad0b4d68a9f56dbfec31ff882025-01-02T22:36:18ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532023-12-013941851891437Detection of bovine coronavirus (bcov) infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animalsMeltem Menekse0Atilla Simsek1Ceren Animal Health Laboratory, Konya, TürkiyeSelcuk University, Veterinary Faculty, Department of Virology, Konya, Türkiye<b>Aim: </b>It was aimed to reveal the antigenic and serological prevalence of bovine coronavirus (BCoV) in cattle with clinically respiratory infection and to evaluate the relationship between virus shedding and serological status of animals in this study.<p> <b>Materials and Methods:</b> In the study, nasal swab samples were collected from a total of 92 cattle of different gender between 0 and 2 years of age with clinical respiratory symptoms. These samples were subjected to RT-PCR using specific primer pairs. Furthermore, serum samples were also collected from the same animals and evaluated for BCoV-specific antibodies through an indirect ELISA method.<p> <b>Results:</b> Ten (10.87%) swap samples were defined as positive for BCoV using RT-PCR. The seropositivity rate was 93.48% (86/92) in cattle by ELISA and 90% (9/10) in virus-shedding animals with nasal swab samples.<p> <b>Conclusion:</b> The fact that 93.47% seropositivity was detected in unvaccinated animals against coronavirus in this study indicates that the animals were exposed to BCoV through colostrum or at a certain period of their lives. However the fact that 9 out of 10 animals with BCoV detected in swab samples also have BCoV-specific antibodies casts doubt on the efficacy of these antibodies in protecting against respiratory BCoV infections. We concluded that more detailed studies are needed to reveal the role of antibodies in respiratory coronavirus infections.http://eurasianjvetsci.org/pdf.php3?id=1437respiratory bcovrt-pcrserology |
| spellingShingle | Meltem Menekse Atilla Simsek Detection of bovine coronavirus (bcov) infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animals Eurasian Journal of Veterinary Sciences respiratory bcov rt-pcr serology |
| title | Detection of bovine coronavirus (bcov) infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animals |
| title_full | Detection of bovine coronavirus (bcov) infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animals |
| title_fullStr | Detection of bovine coronavirus (bcov) infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animals |
| title_full_unstemmed | Detection of bovine coronavirus (bcov) infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animals |
| title_short | Detection of bovine coronavirus (bcov) infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animals |
| title_sort | detection of bovine coronavirus bcov infection in cattle with clinical respiratory signs by pcr and investigation of the serological status of these animals |
| topic | respiratory bcov rt-pcr serology |
| url | http://eurasianjvetsci.org/pdf.php3?id=1437 |
| work_keys_str_mv | AT meltemmenekse detectionofbovinecoronavirusbcovinfectionincattlewithclinicalrespiratorysignsbypcrandinvestigationoftheserologicalstatusoftheseanimals AT atillasimsek detectionofbovinecoronavirusbcovinfectionincattlewithclinicalrespiratorysignsbypcrandinvestigationoftheserologicalstatusoftheseanimals |